Pharmacokinetics of azithromycin and erythromycin in human endometrial epithelial cells and in cells infected with Chlamydia trachomatis

1994 ◽  
Vol 34 (5) ◽  
pp. 765-776 ◽  
Author(s):  
Jane E. Raulston
Keyword(s):  
Chlamydia Trachomatis ◽  
Epithelial Cells ◽  
Endometrial Epithelial Cells ◽  
In Cells

Estrogen enhances attachment of Chlamydia trachomatis to human endometrial epithelial cells in vitro

1988 ◽  
Vol 159 (4) ◽  
pp. 1006-1014 ◽  
Author(s):  
Arthur S. Maslow ◽  
Carolyn H. Davis ◽  
John Choong ◽  
Priscilla B. Wyrick
Keyword(s):  
Chlamydia Trachomatis ◽  
Epithelial Cells ◽  
Endometrial Epithelial Cells

scholarly journals The multifaceted role of oestrogen in enhancing Chlamydia trachomatis infection in polarized human endometrial epithelial cells

2011 ◽  
Vol 13 (8) ◽  
pp. 1183-1199 ◽  
Author(s):  
Jennifer Vanover Hall ◽  
Maria Schell ◽  
Sophie Dessus-Babus ◽  
Cheryl G. Moore ◽  
Judy D. Whittimore ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Epithelial Cells ◽  
Chlamydia Trachomatis Infection ◽  
Endometrial Epithelial Cells

scholarly journals Lactobacillus Rhamnosus GR-1 Ameliorates Trueperella Pyogenes–Induced Barrier Dysfunction of Bovine Endometrial Epithelial Cells

2020 ◽  
Author(s):  
Ning Liu ◽  
Xuan Wu ◽  
Mengling Wang ◽  
Yanan Li ◽  
Le Xu ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Morphological Changes ◽  
Lactobacillus Rhamnosus ◽  
Tunel Staining ◽  
Barrier Dysfunction ◽  
Trueperella Pyogenes ◽  
Effector Caspase ◽  
Wide Range ◽  
Endometrial Epithelial Cells ◽  
In Cells

Trueperella pyogenes is a common opportunistic pathogen which is one of the main causes of postpartum endometritis in dairy cows. As a substitute for antibiotics, the probiotic Lactobacillus rhamnosus GR-1 has been used in a wide range of clinical treatments. Our experiments were designed to establish a model of anti-damage which LGR-1 was used to protect bovine endometrial epithelial cells (BEECs) from inflammatory damage and cell destruction caused by T. pyogenes. Increased expression of NLRP3 inflammasomes and cytokines was observed following T. pyogenes challenge, but this increase was relieved by LGR-1 pretreatment. Immunofluorescence and Western blot analyses revealed that T. pyogenes infection also results in the damage of tight junction proteins in BEECs. The expression levels of Claudin-1, Occludin, and ZO-1 were decreased in cells only infected with T. pyogenes but not in cells pretreated with LGR-1. Moreover, the detection of the anti-apoptotic protein Bcl-2 and apoptotic proteins BAX, cytochrome c, as well as the activating effector caspase-3 revealed that T. pyogenes induced apoptosis of BEECs, which was also confirmed by DAPI staining to observe the morphological changes of the nuclei of cell apoptosis and by TUNEL staining to locate the cells undergoing apoptosis. Our data indicate that LGR-1 ameliorates the T. pyogenes–induced barrier dysfunction of BEECs and pre-application of LGR-1 could be an effective strategy for controlling T. pyogenes infection.

scholarly journals Campylobacter fetus Induced Proinflammatory Response in Bovine Endometrial Epithelial Cells

2021 ◽  
Vol 70 (1) ◽  
pp. 99-106
Author(s):  
LIZETH GUADALUPE CAMPOS-MÚZQUIZ ◽  
ESTELA TERESITA MÉNDEZ-OLVERA ◽  
MONIKA PALACIOS MARTÍNEZ ◽  
DANIEL MARTÍNEZ-GÓMEZ
Keyword(s):  
Epithelial Cells ◽  
Proinflammatory Response ◽  
Expression Levels ◽  
Campylobacter Fetus ◽  
Tnf Α ◽  
Fetus Subsp ◽  
Ifn Γ ◽  
Intracellular Invasion ◽  
Endometrial Epithelial Cells ◽  
In Cells

Campylobacter fetus subsp. fetus is the causal agent of sporadic abortion in bovines and infertility that produces economic losses in livestock. In many infectious diseases, the immune response has an important role in limiting the invasion and proliferation of bacterial patho¬gens. Innate immune sensing of microorganisms is mediated by pattern-recognition receptors (PRRs) that identify pathogen-associated molecular patterns (PAMPs) and induces the secretion of several proinflammatory cytokines, like IL-1β, TNF-α, and IL-8. In this study, the expression of IL-1β, TNF-α, IL-8, and IFN-γ in bovine endometrial epithelial cells infected with C. fetus and Salmonella Typhimurium (a bacterial invasion control) was analyzed. The results showed that expression levels of IL-1β and IL-8 were high at the beginning of the infection and decreased throughout the intracellular period. Unlike in this same assay, the expression levels of IFN-γ increased through time and reached the highest peak at 4 hours post infection. In cells infected with S. Typhimurium, the results showed that IL8 expression levels were highly induced by infection but not IFN-γ. In cells infected with S. Typhimurium or C. fetus subsp. fetus, the results showed that TNF-α expression did not show any change during infection. A cytoskeleton inhibition assay was performed to determine if cytokine expression was modified by C. fetus subsp. fetus intracellular invasion. IL-1β and IL-8 expression were downregulated when an intracellular invasion was avoided. The results obtained in this study suggest that bovine endometrial epithelial cells could recognize C. fetus subsp. fetus resulting in early proinflammatory response.

scholarly journals Protein Disulfide Isomerase, a Component of the Estrogen Receptor Complex, Is Associated with Chlamydia trachomatis Serovar E Attached to Human Endometrial Epithelial Cells

2002 ◽  
Vol 70 (7) ◽  
pp. 3413-3418 ◽  
Author(s):  
C. H. Davis ◽  
J. E. Raulston ◽  
P. B. Wyrick
Keyword(s):  
Estrogen Receptor ◽  
Chlamydia Trachomatis ◽  
Epithelial Cells ◽  
Host Cell ◽  
Protein Disulfide Isomerase ◽  
Host Cells ◽  
Receptor Complex ◽  
Disulfide Isomerase ◽  
Protein Disulfide ◽  
Endometrial Epithelial Cells

ABSTRACT Chlamydia trachomatis serovar E, the leading bacterial agent responsible for sexually transmitted diseases, is required to invade genital epithelial cells for its growth and survival, yet little is known about the adhesin-receptor interactions promoting its entry. In contrast, much has been published on the heparan sulfate receptor for binding C. trachomatis L2 elementary bodies (EBs) prior to entry into HeLa cells. Using a different experimental approach in which a biotinylated apical membrane protein receptor(s) attached to EB at 4°C was stripped off the surface of polarized HEC-1B cells and immunoprecipitated with polyclonal anti-EB antibodies, an ∼55-kDa protein was reproducibly detected by enhanced chemiluminescence and two-dimensional gel electrophoresis. Matrix-assisted laser desorption ionization mass-spectrometry sequence analysis revealed the 55-kDa protein to be protein disulfide isomerase (PDI), a member of the estrogen receptor complex which carries out thiol-disulfide exchange reactions at infected host cell surfaces. Exposure of HEC-1B cells during EB attachment (1.5 to 2 h) to three different inhibitors of PDI reductive reactions—(i) the thiol-alkylating reagent DTNB (5,5′-dithiobis[2-nitrobenzoic acid]), (ii) bacitracin, and (iii) anti-PDI antibodies—resulted in reduced chlamydial infectivity. Since (i) C. trachomatis serovar E attachment to estrogen-dominant primary human endometrial epithelial cells is dramatically enhanced and (ii) productive entry into and infectivity of EB in host cells is dependent on reduction of EB cross-linked outer membrane proteins at the host cell surface, these data provide some preliminary evidence for an intriguing new potential receptor candidate for further analysis of luminal C. trachomatis serovar E entry.

Heat stress induces oxidative stress and activates the KEAP1-NFE2L2-ARE pathway in bovine endometrial epithelial cells

2021 ◽  
Author(s):  
Hirona Murata ◽  
Hiroki Kunii ◽  
Kazuya Kusama ◽  
Toshihiro Sakurai ◽  
Hanako Bai ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Heat Stress ◽  
High Temperature ◽  
Epithelial Cells ◽  
Quantitative Polymerase Chain Reaction ◽  
Luciferase Reporter ◽  
Expression Levels ◽  
Endometrial Epithelial Cells ◽  
Cells Cultured ◽  
In Cells

Abstract Heat stress adversely affects the reproductive function in cows. Although a relationship between heat stress and oxidative stress has been suggested, it has not been sufficiently verified in bovine endometrial epithelial cells. Here, we investigated whether oxidative stress is induced by heat stress in bovine endometrial epithelial cells under high temperature. Luciferase reporter assays showed that the reporter activity of heat shock element (HSE) and antioxidant responsive element (ARE) was increased in endometrial epithelial cells cultured under high temperature compared to that in cells cultured under basal (thermoneutral) temperature. Also, nuclear factor, erythroid 2 like 2 (NFE2L2), a master regulator of cellular environmental stress response, stabilized and the expression levels of antioxidant enzyme genes increased under high temperature. Immunostaining confirmed the nuclear localization of NFE2L2 in endometrial epithelial cells cultured under high temperature. Quantitative polymerase chain reaction analysis showed that the expression levels of representative inflammatory cytokine genes, such as prostaglandin-endoperoxide synthase 2 (PTGS2) and interleukin 8, were significantly decreased in endometrial epithelial cells cultured under high temperature compared to those in cells cultured under basal temperature. Thus, our results suggest that heat stress induces oxidative stress, whereas NFE2L2 plays a protective role in bovine endometrial epithelial cells cultured under heat stress conditions.

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