Multi-resistance isolates possessing characteristics of both Burkholderia (Pseudomonas) cepacia and Burkholderia gladioli from patients with cystic fibrosis

1994 ◽  
Vol 34 (3) ◽  
pp. 353-361 ◽  
Author(s):  
I. N. Simpson ◽  
J. Finlay ◽  
D. J. Winstanley ◽  
N. Dewhurst ◽  
J. W. Nelson ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Cepacia ◽  
Burkholderia Gladioli

Person-to-person transmission of Pseudomonas cepacia between patients with cystic fibrosis

The Lancet ◽  
1990 ◽  
Vol 336 (8723) ◽  
pp. 1094-1096 ◽  
Author(s):  
J.J. LiPuma ◽  
S.E. Dasen ◽  
T.L. Stull ◽  
D.W. Nielson ◽  
R.C. Stern
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Cepacia

Pseudomonas cepacia infection in cystic fibrosis

The Lancet ◽  
1992 ◽  
Vol 339 (8787) ◽  
pp. 252 ◽  
Author(s):  
D.L. Smith ◽  
E.G. Smith ◽  
L.B. Gumery ◽  
D.E. Stableforth
Keyword(s):  
Cystic Fibrosis ◽  
Pseudomonas Cepacia

Burkholderia Gladioli – A Predictor Of Poor Outcome In Cystic Fibrosis Patients Who Receive Lung Transplants?

2010 ◽  
Author(s):  
Bradley S. Quon ◽  
James D. Reid ◽  
Patrick H. Wong ◽  
Pearce G. Wilcox ◽  
Amin R. Javer ◽  
...  
Keyword(s):  
Cystic Fibrosis ◽  
Poor Outcome ◽  
Burkholderia Gladioli ◽  
Lung Transplants

scholarly journals Nucleotide sequence analysis of a gene from Burkholderia (Pseudomonas) cepacia encoding an outer membrane lipoprotein involved in multiple antibiotic resistance.

1996 ◽  
Vol 40 (2) ◽  
pp. 307-313 ◽  
Author(s):  
J L Burns ◽  
C D Wadsworth ◽  
J J Barry ◽  
C P Goodall
Keyword(s):  
Cystic Fibrosis ◽  
Antibiotic Resistance ◽  
Outer Membrane ◽  
Open Reading Frame ◽  
Pseudomonas Cepacia ◽  
Multiple Antibiotic Resistance ◽  
Reading Frame ◽  
Outer Membrane Lipoprotein ◽  
Membrane Lipoprotein ◽  
Antibiotic Efflux

Antibiotic-resistant Burkholderia (Pseudomonas) cepacia is an important etiologic agent of nosocomial and cystic fibrosis infections. The primary resistance mechanism which has been reported is decreased outer membrane permeability. We previously reported the cloning and characterization of a chloramphenicol resistance determinant from an isolate of B. cepacia from a patient with cystic fibrosis that resulted in decreased drug accumulation. In the present studies we subcloned and sequenced the resistance determinant and identified gene products related to decreased drug accumulation. Additional drug resistances encoded by the determinant include resistances to trimethoprim and ciprofloxacin. Sequence analysis of a 3.4-kb subcloned fragment identified one complete and one partial open reading frame which are homologous with two of three components of a potential antibiotic efflux operon from Pseudomonas aeruginosa (mexA-mexB-oprM). On the basis of sequence data, outer membrane protein analysis, protein expression systems, and a lipoprotein labelling assay, the complete open reading frame encodes an outer membrane lipoprotein which is homologous with OprM. The partial open reading frame shows homology at the protein level with the C terminus of the protein product of mexB. DNA hybridization studies demonstrated homology of an internal mexA probe with a larger subcloned fragment from B. cepacia. The finding of multiple antibiotic resistance in B. cepacia as a result of an antibiotic efflux pump is surprising because it has long been believed that resistance in this organism is caused by impermeability to antibiotics.

scholarly journals Isolation of Pseudomonas cepacia in cystic fibrosis patient

1993 ◽  
Vol 88 (1) ◽  
pp. 125-129 ◽  
Author(s):  
Elizabeth de Andrade Marques ◽  
Rosa Maria Carvalho Pinto ◽  
Ludma Trotta Dallallana ◽  
Elsa Fuchshuber Rodrigues de Oliveira ◽  
Italo Suassuna
Keyword(s):  
Cystic Fibrosis ◽  
Cystic Fibrosis Patient ◽  
Pseudomonas Cepacia

scholarly journals Species-Specific PCR as a Tool for the Identification of Burkholderia gladioli

2000 ◽  
Vol 38 (1) ◽  
pp. 282-285
Author(s):  
Paul W. Whitby ◽  
Lauren C. Pope ◽  
Karen B. Carter ◽  
John J. LiPuma ◽  
Terrence L. Stull
Keyword(s):  
Cystic Fibrosis ◽  
Sensitivity And Specificity ◽  
Burkholderia Cepacia ◽  
Bacterial Species ◽  
23S Rrna ◽  
Computer Assisted ◽  
Accurate Identification ◽  
Specific Pcr ◽  
Burkholderia Gladioli ◽  
Species Specific

ABSTRACT Burkholderia gladioli colonizes the respiratory tracts of patients with cystic fibrosis and chronic granulomatous disease. However, due to the high degree of phenotypic similarity between this species and closely related species in the Burkholderia cepacia complex, accurate identification is difficult. Incorrect identification of these species may have serious repercussions for the management of patients with cystic fibrosis. To develop an accurate procedure for the identification of B. gladioli , a molecular method to discriminate between this species and other species commonly isolated from the sputa of patients with cystic fibrosis was investigated. The 23S ribosomal DNA was cloned from several clinical isolates of B. gladioli , and the nucleotide sequence was determined. Computer-assisted sequence comparisons indicated four regions of the 23S rRNA specific for this species; these regions were used to design three primer pairs for species-specific PCR. Two of the primer pairs showed 100% sensitivity and specificity for B. gladioli when tested against a panel of 47 isolates comprising 19 B. gladioli isolates and 28 isolates of 16 other bacterial species. One of the primer pairs was further assessed for species specificity by using a panel of 102 isolates obtained from the Burkholderia cepacia Research Laboratory and Repository. The species-specific PCR was positive for 70 of 74 isolates of B. gladioli and was negative for all other bacterial species examined. Overall, this primer pair displayed a sensitivity and specificity of 96% (89 of 93) and 100%, respectively. These data demonstrate the potential of species-specific PCR for the identification of B. gladioli .

scholarly journals Airborne dissemination of Burkholderia (Pseudomonas) cepacia from adult patients with cystic fibrosis.

Thorax ◽  
1994 ◽  
Vol 49 (11) ◽  
pp. 1157-1159 ◽  
Author(s):  
H Humphreys ◽  
D Peckham ◽  
P Patel ◽  
A Knox
Keyword(s):  
Cystic Fibrosis ◽  
Adult Patients ◽  
Pseudomonas Cepacia
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