In-vitro activity of the new triazole D0870 compared with amphotericin B and itraconazole against Aspergilus spp.

1993 ◽  
Vol 32 (6) ◽  
pp. 831-836 ◽  
Author(s):  
Caroline B. Moore ◽  
Derek Law ◽  
David W. Denning
Keyword(s):  
Amphotericin B ◽  
Vitro Activity ◽  
In Vitro Activity

In vitro activity of olorofim against clinical isolates of Scedosporium species and Lomentospora prolificans using EUCAST and CLSI methodologies

2020 ◽  
Vol 75 (12) ◽  
pp. 3582-3585
Author(s):  
Olga Rivero-Menendez ◽  
Manuel Cuenca-Estrella ◽  
Ana Alastruey-Izquierdo
Keyword(s):  
Amphotericin B ◽  
Susceptibility Testing ◽  
Antifungal Susceptibility ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
Scedosporium Apiospermum ◽  
In Vitro Activity ◽  
Scedosporium Dehoogii ◽  
Scedosporium Species

Abstract Objectives To evaluate the in vitro activity of olorofim, a new broad-spectrum antifungal with a novel mechanism of action, against a collection of 123 Spanish clinical isolates belonging to five Scedosporium species and Lomentospora prolificans. Methods The activity of olorofim against Scedosporium apiospermum (n = 30), Scedosporium boydii (n = 30), Scedosporium ellipsoideum (n = 10), Scedosporium aurantiacum (n = 20), Scedosporium dehoogii (n = 3) and Lomentospora prolificans (n = 30) was compared with that of amphotericin B, voriconazole, isavuconazole and micafungin by performing EUCAST and CLSI reference methods for antifungal susceptibility testing. Results Amphotericin B and isavuconazole showed MICs ≥2 mg/L for all the species evaluated and voriconazole was moderately active (GM, MIC50 and MIC90 values ≤2 mg/L) against all of them except L. prolificans. Micafungin was effective against S. apiospermum complex strains, but exhibited elevated MECs for S. dehoogii and S. aurantiacum. Olorofim showed low MICs for all the Scedosporium strains tested (GM values were lower than 0.130 and 0.339 by the EUCAST method and the CLSI method, respectively, for all of the species), including those belonging to the MDR species L. prolificans, for which GM values were 0.115 and 0.225 mg/L by the EUCAST method and the CLSI method, respectively, while the GMs for the rest of the antifungals evaluated were higher than 3.732 mg/L using both methodologies. Conclusions Olorofim displayed promising in vitro activity against the Scedosporium and L. prolificans strains tested, some of which have reduced susceptibility to the antifungals that are currently in use.

scholarly journals In Vitro Activity of Nystatin Compared with Those of Liposomal Nystatin, Amphotericin B, and Fluconazole against Clinical Candida Isolates

2002 ◽  
Vol 40 (4) ◽  
pp. 1406-1412 ◽  
Author(s):  
S. Arikan ◽  
L. Ostrosky-Zeichner ◽  
M. Lozano-Chiu ◽  
V. Paetznick ◽  
D. Gordon ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Vitro Activity ◽  
In Vitro Activity

In vitro activity of voriconazole, itraconazole, caspofungin, anidulafungin (VER002, LY303366) and amphotericin B against aspergillus spp

2003 ◽  
Vol 45 (2) ◽  
pp. 131-135 ◽  
Author(s):  
María del Carmen Serrano ◽  
Anastasio Valverde-Conde ◽  
M.ónica Chávez ◽  
Samuel Bernal ◽  
Rosa María Claro ◽  
...  
Keyword(s):  
Amphotericin B ◽  
Vitro Activity ◽  
In Vitro Activity

scholarly journals Comparison of In Vitro Activity of Liposomal Nystatin againstAspergillus Species with Those of Nystatin, Amphotericin B (AB) Deoxycholate, AB Colloidal Dispersion, Liposomal AB, AB Lipid Complex, and Itraconazole

1999 ◽  
Vol 43 (5) ◽  
pp. 1264-1266 ◽  
Author(s):  
Karen L. Oakley ◽  
Caroline B. Moore ◽  
David W. Denning
Keyword(s):  
Amphotericin B ◽  
Aspergillus Terreus ◽  
Antifungal Agents ◽  
Geometric Mean ◽  
Colloidal Dispersion ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Lipid Complex

ABSTRACT We compared the in vitro activity of liposomal nystatin (Nyotran) with those of other antifungal agents against 60Aspergillus isolates. Twelve isolates were itraconazole resistant. For all isolates, geometric mean (GM) MICs (micrograms per milliliter) were 2.30 for liposomal nystatin, 0.58 for itraconazole, 0.86 for amphotericin B (AB) deoxycholate, 9.51 for nystatin, 2.07 for liposomal AB, 2.57 for AB lipid complex, and 0.86 for AB colloidal dispersion. Aspergillus terreus (GM, 8.72 μg/ml; range, 8 to 16 μg/ml) was significantly less susceptible to all of the polyene drugs than all other species (P = 0.0001).

scholarly journals In vitro activity of a new echinocandin, LY303366, compared with those of amphotericin B and fluconazole against clinical yeast isolates.

1997 ◽  
Vol 41 (5) ◽  
pp. 1156-1157 ◽  
Author(s):  
O Uzun ◽  
S Kocagöz ◽  
Y Cetinkaya ◽  
S Arikan ◽  
S Unal
Keyword(s):  
Candida Albicans ◽  
Amphotericin B ◽  
Vitro Activity ◽  
In Vitro Activity ◽  
Broth Microdilution ◽  
Microdilution Method ◽  
Broth Microdilution Method

The in vitro activity of LY303366, a new echinocandin derivative, was evaluated with 191 yeast isolates by a broth microdilution method. The MICs at which 50% of the isolates were inhibited were 0.125 microg/ml for Candida albicans and C. tropicalis, 0.25 microg/ml for C. krusei, C. kefyr, and C. glabrata, and 2.0 microg/ml for C. parapsilosis.

scholarly journals Relationship between In Vitro Activities of Amphotericin B and Flucytosine and pH for Clinical Yeast and Mold Isolates

2005 ◽  
Vol 49 (8) ◽  
pp. 3341-3346 ◽  
Author(s):  
D. T. A. te Dorsthorst ◽  
P. E. Verweij ◽  
J. F. G. M. Meis ◽  
J. W. Mouton
Keyword(s):  
Amphotericin B ◽  
Phosphate Buffer ◽  
Vitro Activity ◽  
Nonlinear Relationship ◽  
In Vitro Activity ◽  
The Mean ◽  
Ph Range ◽  
Citrate Phosphate ◽  
Citrate Phosphate Buffer

ABSTRACT In this study, we investigated the pH dependency of the in vitro activities of amphotericin B (AMB) and flucytosine (5FC) against Candida spp., Cryptococcus neoformans, Aspergillus fumigatus, Rhizopus spp., and Scedosporium prolificans in RPMI 1640 buffered with citrate buffer (pH 4.0, 5.0, 5.4, and 6.0), citrate-phosphate buffer (pH 5.4, 6.0, 6.4, and 7.0), and 3-[N-morpholino]propanesulfonic acid (MOPS) (pH 6.4, 7.0, 7.4, and 7.9). For 5FC, no significant differences were found between MICs obtained with the different buffers, while for AMB, significant differences were found. The MICs obtained with citrate-phosphate buffer were approximately 1 twofold-dilution step higher than the MICs obtained with MOPS. We demonstrated that the in vitro activities of AMB and 5FC against yeast and mold isolates were pH dependent. The in vitro activity of AMB decreased when the pH was lowered, while the in vitro activity of 5FC increased. The effect of the pH on the in vitro activities was dependent not only on the antifungal agent tested but also on the microorganism. For AMB, there was a nonlinear relationship (median r 2, 0.864) for Candida spp., C. neoformans, A. fumigatus, and Rhizopus spp. over the pH range tested. The mean MICs ranged from 0.5 to 2.52 μg/ml at pH 7.0 and from 20.16 to 32 μg/ml at pH 5.0. For S. prolificans, there was no relationship. For 5FC, there was a linear relationship for Candida spp. (median r 2, 0.767) and a nonlinear relationship for C. neoformans and A. fumigatus (median r 2, 0.882) over the pH range tested. The mean MIC values ranged from 0.125 to 1,024 μg/ml at pH 7.0 and from 0.02 to 4 μg/ml at pH 5.0. For Rhizopus spp. and S. prolificans, the relationship could not be determined, since the MIC was >1,024 μg/ml over a pH range of 4.0 to 7.9.

scholarly journals Enhancement of the in vitro activity of amphotericin B against Aspergillus spp. by tetracycline analogs.

1984 ◽  
Vol 26 (6) ◽  
pp. 837-840 ◽  
Author(s):  
C E Hughes ◽  
C Harris ◽  
L R Peterson ◽  
D N Gerding
Keyword(s):  
Amphotericin B ◽  
Vitro Activity ◽  
In Vitro Activity

scholarly journals In Vitro Activities of Terbinafine againstAspergillus Species in Comparison with Those of Itraconazole and Amphotericin B

2001 ◽  
Vol 45 (6) ◽  
pp. 1882-1885 ◽  
Author(s):  
Caroline B. Moore ◽  
Caroline M. Walls ◽  
David W. Denning
Keyword(s):  
Aspergillus Fumigatus ◽  
Amphotericin B ◽  
Geometric Mean ◽  
Vitro Activity ◽  
Clinical Correlation ◽  
In Vitro Activity

ABSTRACT Compared with the in vitro activities of itraconazole (geometric mean MIC [GM], 0.56 μg/ml) and amphotericin B (GM, 0.66 μg/ml), the in vitro activity of terbinafine was inferior againstAspergillus fumigatus (GM, 19.03 μg/ml) (P < 0.05) and superior against A. flavus(GM, 0.10 μg/ml), A. terreus (GM, 0.16 μg/ml), andA. niger (GM, 0.19 μg/ml). Clinical correlation is required, as trailing endpoints are problematic.

scholarly journals Comparison of the in-vitro activity of voriconazole (UK-109,496), itraconazole and amphotericin B against clinical isolates of Aspergillus fumigatus

1998 ◽  
Vol 42 (4) ◽  
pp. 531-533 ◽  
Author(s):  
M. Cuenca-Estrella ◽  
J. L. Rodriguez-Tudela ◽  
E. Mellado ◽  
J. V. Martinez-Suarez ◽  
A. Monzon
Keyword(s):  
Aspergillus Fumigatus ◽  
Amphotericin B ◽  
Vitro Activity ◽  
Clinical Isolates ◽  
In Vitro Activity
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