scholarly journals Identification of Phase-Variable Genes That May Contribute to Nontypeable Haemophilus influenzae Nasopharyngeal Colonization in Humans Contributes to Our Understanding of Specific Host-Pathogen Interactions

2013 ◽  
Vol 208 (5) ◽  
pp. 713-716 ◽  
Author(s):  
Thomas J. Inzana
Keyword(s):  
Haemophilus Influenzae ◽  
Phase Variable ◽  
Nontypeable Haemophilus Influenzae ◽  
Host Pathogen Interactions ◽  
Nasopharyngeal Colonization ◽  
Specific Host ◽  
Host Pathogen ◽  
Variable Genes

scholarly journals Analysis of Nontypeable Haemophilus influenzae Phase-Variable Genes During Experimental Human Nasopharyngeal Colonization

2013 ◽  
Vol 208 (5) ◽  
pp. 720-727 ◽  
Author(s):  
Jessica Poole ◽  
Eric Foster ◽  
Kathryn Chaloner ◽  
Jason Hunt ◽  
Michael P. Jennings ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Phase Variable ◽  
Nontypeable Haemophilus Influenzae ◽  
Nasopharyngeal Colonization ◽  
Variable Genes

Epidemiology of Nasopharyngeal Colonization with Nontypeable Haemophilus influenzae in the First 2 Years of Life

1995 ◽  
Vol 172 (1) ◽  
pp. 132-135 ◽  
Author(s):  
H. Faden ◽  
L. Duffy ◽  
A. Williams ◽  
D. A. Krystofik ◽  
J. Wolf ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Nontypeable Haemophilus Influenzae ◽  
Nasopharyngeal Colonization

scholarly journals Plasmodium vivax Cell Traversal Protein for Ookinetes and Sporozoites (CelTOS) Functionally Restricted Regions Are Involved in Specific Host-Pathogen Interactions

2020 ◽  
Vol 10 ◽  
Author(s):  
Gabriela Arévalo-Pinzón ◽  
Diego Garzón-Ospina ◽  
Fredy A. Pulido ◽  
Maritza Bermúdez ◽  
Johanna Forero-Rodríguez ◽  
...  
Keyword(s):  
Plasmodium Vivax ◽  
Host Pathogen Interactions ◽  
Specific Host ◽  
Host Pathogen

scholarly journals Highly specific host-pathogen interactions influence Metarhizium brunneum blastospore virulence against Culex quinquefasciatus larvae

Virulence ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 1449-1467 ◽  
Author(s):  
Abeer M. Alkhaibari ◽  
Alex M. Lord ◽  
Thierry Maffeis ◽  
James C. Bull ◽  
Fabio L. Olivares ◽  
...  
Keyword(s):  
Culex Quinquefasciatus ◽  
Host Pathogen Interactions ◽  
Metarhizium Brunneum ◽  
Specific Host ◽  
Host Pathogen

scholarly journals Multiple Consecutive Lavage Samplings Reveal Greater Burden of Disease and Provide Direct Access to the Nontypeable Haemophilus influenzae Biofilm in Experimental Otitis Media

2007 ◽  
Vol 75 (8) ◽  
pp. 4158-4172 ◽  
Author(s):  
Magali Leroy ◽  
Howard Cabral ◽  
Marisol Figueira ◽  
Valérie Bouchet ◽  
Heather Huot ◽  
...  
Keyword(s):  
Gene Expression ◽  
Haemophilus Influenzae ◽  
Otitis Media ◽  
Vaccine Development ◽  
Ex Vivo ◽  
Direct Analysis ◽  
Direct Access ◽  
Nontypeable Haemophilus Influenzae ◽  
Nasopharyngeal Colonization

ABSTRACT The typically recovered quantity of nontypeable Haemophilus influenzae (NTHi) bacteria in an ex vivo middle ear (ME) aspirate from the chinchilla model of experimental otitis media is insufficient for direct analysis of gene expression by microarray or of lipopolysaccharide glycoforms by mass spectrometry. This prompted us to investigate a strategy of multiple consecutive lavage samplings to increase ex vivo bacterial recovery. As multiple consecutive lavage samples significantly increased the total number of bacterial CFU collected during nasopharyngeal colonization or ME infection, this led us to evaluate whether bacteria sequentially acquired from consecutive lavages were similar. Comparative observation of complete ex vivo sample series by microscopy initially revealed ME inflammatory fluid consisting solely of planktonic-phase NTHi. In contrast, subsequent lavage samplings of the same infected ear revealed the existence of bacteria in two additional growth states, filamentous and biofilm encased. Gene expression analysis of such ex vivo samples was in accord with different bacterial growth phases in sequential lavage specimens. The existence of morphologically distinct NTHi subpopulations with varying levels of gene expression indicates that the pooling of specimens requires caution until methods for their separation are developed. This study based on multiple consecutive lavages is consistent with prior reports that NTHi forms a biofilm in vivo, describes the means to directly acquire ex vivo biofilm samples without sacrificing the animal, and has broad applicability for a study of mucosal infections. Moreover, this approach revealed that the actual burden of bacteria in experimental otitis media is significantly greater than was previously reported. Such findings may have direct implications for antibiotic treatment and vaccine development against NTHi.

Uncovering complex molecular networks in host–pathogen interactions using systems biology

2019 ◽  
Vol 3 (4) ◽  
pp. 371-378
Author(s):  
Joshua M. Peters ◽  
Sydney L. Solomon ◽  
Christopher Y. Itoh ◽  
Bryan D. Bryson
Keyword(s):  
Systems Biology ◽  
High Throughput ◽  
Cell Biology ◽  
Molecular Networks ◽  
Host Pathogen Interactions ◽  
Exciting Field ◽  
Specific Host ◽  
Biomolecular Network ◽  
Host Pathogen ◽  
Analytical Frameworks

Abstract Interactions between pathogens and their hosts can induce complex changes in both host and pathogen states to privilege pathogen survival or host clearance of the pathogen. To determine the consequences of specific host–pathogen interactions, a variety of techniques in microbiology, cell biology, and immunology are available to researchers. Systems biology that enables unbiased measurements of transcriptomes, proteomes, and other biomolecules has become increasingly common in the study of host–pathogen interactions. These approaches can be used to generate novel hypotheses or to characterize the effects of particular perturbations across an entire biomolecular network. With proper experimental design and complementary data analysis tools, high-throughput omics techniques can provide novel insights into the mechanisms that underlie processes from phagocytosis to pathogen immune evasion. Here, we provide an overview of the suite of biochemical approaches for high-throughput analyses of host–pathogen interactions, analytical frameworks for understanding the resulting datasets, and a vision for the future of this exciting field.

scholarly journals Closed Complete Genome Sequences of Two Nontypeable Haemophilus influenzae Strains Containing Novel modA Alleles from the Sputum of Patients with Chronic Obstructive Pulmonary Disease

2018 ◽  
Vol 7 (2) ◽  
Author(s):  
John M. Atack ◽  
Timothy F. Murphy ◽  
Lauren O. Bakaletz ◽  
Kate L. Seib ◽  
Michael P. Jennings
Keyword(s):  
Chronic Obstructive Pulmonary Disease ◽  
Haemophilus Influenzae ◽  
Pulmonary Disease ◽  
Complete Genome ◽  
Chronic Obstructive ◽  
Phase Variable ◽  
Nontypeable Haemophilus Influenzae ◽  
Genome Sequences ◽  
Obstructive Pulmonary Disease ◽  
Content Type

Nontypeable Haemophilus influenzae (NTHi) is an important bacterial pathogen that causes otitis media and exacerbations of chronic obstructive pulmonary disease (COPD). Here, we report the complete genome sequences of NTHi strains 10P129H1 and 84P36H1, isolated from COPD patients, which contain the phase-variable epigenetic regulators ModA15 and ModA18, respectively.

scholarly journals Erratum for Baddal et al., Dual RNA-seq of Nontypeable Haemophilus influenzae and Host Cell Transcriptomes Reveals Novel Insights into Host-Pathogen Cross Talk

mBio ◽  
2016 ◽  
Vol 7 (2) ◽  
Author(s):  
Buket Baddal ◽  
Alessandro Muzzi ◽  
Stefano Censini ◽  
Raffaele A. Calogero ◽  
Giulia Torricelli ◽  
...  
Keyword(s):  
Haemophilus Influenzae ◽  
Host Cell ◽  
Cross Talk ◽  
Rna Seq ◽  
Nontypeable Haemophilus Influenzae ◽  
Host Pathogen
Sign in / Sign up

Export Citation Format

Share Document