scholarly journals Humoral and Cellular Immune Responses following Vaccination with Purified Recombinant Hemagglutinin from Influenza A (H3N2) Virus

1997 ◽  
Vol 175 (2) ◽  
pp. 342-351 ◽  
Author(s):  
D. C. Powers ◽  
J. E. McElhaney ◽  
O. A. Florendo ◽  
M. C. Manning ◽  
C. M. Upshaw ◽  
...  
Keyword(s):  
Immune Responses ◽  
Influenza A ◽  
H3n2 Virus ◽  
Cellular Immune Responses ◽  
Recombinant Hemagglutinin ◽  
Cellular Immune

Lower cellular immune responses to influenza A (H3N2) in the elderly

2009 ◽  
Vol 81 (8) ◽  
pp. 1471-1476 ◽  
Author(s):  
Na Jia ◽  
Chris Li ◽  
Yun-Xi Liu ◽  
Jan H. Richardus ◽  
Dan Feng ◽  
...  
Keyword(s):  
Immune Responses ◽  
Influenza A ◽  
The Elderly ◽  
Cellular Immune Responses ◽  
Cellular Immune

scholarly journals Baseline Levels of Influenza-Specific B Cells and T Cell Responses Modulate Human Immune Responses to Swine Variant Influenza A/H3N2 Vaccine

Vaccines ◽  
2020 ◽  
Vol 8 (1) ◽  
pp. 126
Author(s):  
Lilin Lai ◽  
Nadine Rouphael ◽  
Yongxian Xu ◽  
Amy C. Sherman ◽  
Srilatha Edupuganti ◽  
...  
Keyword(s):  
T Cells ◽  
T Cell ◽  
Immune Responses ◽  
Influenza A ◽  
Vaccine Dose ◽  
T Cell Responses ◽  
Post Vaccination ◽  
Cell Responses ◽  
Cellular Immune Responses ◽  
Cellular Immune

The cellular immune responses elicited by an investigational vaccine against an emergent variant of influenza (H3N2v) are not fully understood. Twenty-five subjects, enrolled in an investigational influenza A/H3N2v vaccine study, who received two doses of vaccine 21 days apart, were included in a sub-study of cellular immune responses. H3N2v-specific plasmablasts were determined by ELISpot 8 days after each vaccine dose and H3N2v specific CD4+ T cells were quantified by intracellular cytokine and CD154 (CD40 ligand) staining before vaccination, 8 and 21 days after each vaccine dose. Results: 95% (19/20) and 96% (24/25) subjects had pre-existing H3N2v specific memory B, and T cell responses, respectively. Plasmablast responses at Day 8 after the first vaccine administration were detected against contemporary H3N2 strains and correlated with hemagglutination inhibition HAI (IgG: p = 0.018; IgA: p < 0.001) and Neut (IgG: p = 0.038; IgA: p = 0.021) titers and with memory B cell frequency at baseline (IgA: r = 0.76, p < 0.001; IgG: r = 0.74, p = 0.0001). The CD4+ T cells at Days 8 and 21 expanded after prime vaccination and this expansion correlated strongly with early post-vaccination HAI and Neut titers (p ≤ 0.002). In an adult population, the rapid serological response observed after initial H3N2v vaccination correlates with post-vaccination plasmablasts and CD4+ T cell responses.

scholarly journals Novel Bivalent Viral-Vectored Vaccines Induce Potent Humoral and Cellular Immune Responses Conferring Protection against Stringent Influenza A Virus Challenge

2017 ◽  
Vol 199 (4) ◽  
pp. 1333-1341 ◽  
Author(s):  
Claire M. Tully ◽  
Senthil Chinnakannan ◽  
Caitlin E. Mullarkey ◽  
Marta Ulaszewska ◽  
Francesca Ferrara ◽  
...  
Keyword(s):  
Immune Responses ◽  
Influenza A Virus ◽  
Influenza A ◽  
Virus Challenge ◽  
Cellular Immune Responses ◽  
Cellular Immune

scholarly journals An evaluation of cytokine and cellular immune responses to heterologous prime-boost vaccination with influenza A/H7N7-A/H7N9 inactivated vaccine

2020 ◽  
Vol 16 (12) ◽  
pp. 3138-3145 ◽  
Author(s):  
Hana M El Sahly ◽  
George Makedonas ◽  
David Corry ◽  
Robert L Atmar ◽  
Abbie Bellamy ◽  
...  
Keyword(s):  
Immune Responses ◽  
Influenza A ◽  
Inactivated Vaccine ◽  
Cellular Immune Responses ◽  
Boost Vaccination ◽  
Cellular Immune

scholarly journals CpG ODN and ISCOMATRIX Adjuvant: A Synergistic Adjuvant Combination Inducing Strong T-Cell IFN-γResponses

2013 ◽  
Vol 2013 ◽  
pp. 1-11 ◽  
Author(s):  
Michael J. McCluskie ◽  
Risini D. Weeratna ◽  
Dana M. Evans ◽  
Shawn Makinen ◽  
Debbie Drane ◽  
...  
Keyword(s):  
Immune Responses ◽  
Influenza A ◽  
Hepatitis B Surface Antigen ◽  
Tumor Cell Line ◽  
Cpg Odn ◽  
Adjuvant Activity ◽  
Cellular Immune Responses ◽  
Preclinical And Clinical Studies ◽  
Cellular Immune ◽  
Adjuvant Effects

For the induction of robust humoral and cellular immune responses, a strong rationale exists to use vaccine-adjuvant combinations possessing both immune modulatory and enhanced delivery capabilities. Herein, we evaluated the combination of 2 different adjuvants, a TLR9 agonist, composed of synthetic oligodeoxynucleotides (ODN) containing immunostimulatory CpG motifs (CpG), and ISCOMATRIX adjuvant (ISCOMATRIX), composed of saponin, phospholipid, and cholesterol, which possesses both immunostimulatory and delivery properties. While both individual adjuvants have been shown effective in numerous preclinical and clinical studies, it is likely that for optimal adjuvant activity a combined adjuvant approach will be necessary. Herein, using three different antigens, namely, hepatitis B surface antigen (HBsAg), ovalbumin (OVA), and influenza A haemagglutinin antigen (HA), we show in mice that some adjuvant effects of CpG and ISCOMATRIX are further enhanced if they are used in combination. In particular, with all three antigens, IFN-γlevels were greatly increased with the CpG/ISCOMATRIX combination. The ability of the CpG/ISCOMATRIX combination to induce antitumor responses when administered with OVA following administration to mice of a highly metastatic OVA-secreting tumor cell line (B16-OVA melanoma) was also demonstrated. Thus the CpG/ISCOMATRIX combination may prove to be a valuable tool in the development of novel or improved vaccines.

scholarly journals Cellular Immune Responses in Children and Adults Receiving Inactivated or Live Attenuated Influenza Vaccines

2006 ◽  
Vol 80 (23) ◽  
pp. 11756-11766 ◽  
Author(s):  
Xiao-Song He ◽  
Tyson H. Holmes ◽  
Caiqiu Zhang ◽  
Kutubuddin Mahmood ◽  
George W. Kemble ◽  
...  
Keyword(s):  
T Cells ◽  
Immune Responses ◽  
Influenza Vaccine ◽  
Influenza A Virus ◽  
Influenza A ◽  
Influenza Vaccines ◽  
Cellular Immune Responses ◽  
Ifn Γ ◽  
The Mean ◽  
Cellular Immune

ABSTRACT The patterns of cellular immune responses induced by live attenuated influenza vaccine (LAIV) versus those of the trivalent inactivated influenza vaccine (TIV) have not been studied extensively, especially in children. The goals of this study were to evaluate the effects of TIV and LAIV immunization on cellular immunity to live influenza A virus in children and adults and to explore factors associated with variations in responses to influenza vaccines among individuals. A gamma interferon (IFN-γ) flow cytometry assay was used to measure IFN-γ-producing (IFN-γ+) NK and T cells in peripheral blood mononuclear cell cultures stimulated with a live influenza A virus strain before and after LAIV or TIV immunization of children and adults. The mean percentages of influenza A virus-specific IFN-γ+ CD4 and CD8 T cells increased significantly after LAIV, but not TIV, immunization in children aged 5 to 9 years. No increases in the mean levels of influenza A virus-reactive IFN-γ+ T cells and NK cells were observed in adults given LAIV or TIV. TIV induced a significant increase in influenza A virus-reactive T cells in 6-month- to 4-year-old children; LAIV was not evaluated in this age group. The postvaccination changes (n-fold) in the percentages of influenza A virus-reactive IFN-γ+ T and NK cells in adults were highly variable and correlated inversely with the prevaccination percentages, in particular with that of the CD56dim NK cell subset. In conclusion, our findings identify age, type of vaccine, and prevaccination levels of immune reactivity to influenza A virus as factors significantly associated with the magnitude of cellular immune responses to influenza vaccines.

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