Endotoxin and Tumor Necrosis Factor-α-Induced Interleukin-8 Release in Humans

1993 ◽  
Vol 167 (2) ◽  
pp. 461-464 ◽  
Author(s):  
Sander J. H. van Deventer ◽  
Margreet Hart ◽  
Tom van der Poll ◽  
C. Erik Hack ◽  
Lucien A. Aarden
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 8 ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Opposite Regulation of Interleukin-8 and Tumor Necrosis Factor-α by Weight Loss

2002 ◽  
Vol 10 (6) ◽  
pp. 499-506 ◽  
Author(s):  
Jens M. Bruun ◽  
Steen B. Pedersen ◽  
Kurt Kristensen ◽  
Bjørn Richelsen
Keyword(s):  
Weight Loss ◽  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 8 ◽  
Factor Α ◽  
Necrosis Factor

Maternal serum interleukin-6, interleukin-8, tumor necrosis factor-α and interferon-γ in preterm labor

2003 ◽  
Vol 82 (6) ◽  
pp. 543-549
Author(s):  
Ahmed M. Bahar ◽  
Hashim W. Ghalib ◽  
Riyad A. Moosa ◽  
Zaki M. S. Zaki ◽  
Chet Thomas ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Interleukin 6 ◽  
Preterm Labor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interferon Γ ◽  
Maternal Serum ◽  
Interleukin 8 ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Polyamine depletion inhibits NF-κB binding to DNA and interleukin-8 production in human chondrocytes stimulated by tumor necrosis factor-α

2005 ◽  
Vol 204 (3) ◽  
pp. 956-963 ◽  
Author(s):  
Annalisa Facchini ◽  
Rosa Maria BorzÍ ◽  
Kenneth B. Marcu ◽  
Claudio Stefanelli ◽  
Eleonora Olivotto ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 8 ◽  
Human Chondrocytes ◽  
Factor Α ◽  
Necrosis Factor

scholarly journals Tumor Necrosis Factor-α and Interleukin-1β Regulate Interleukin-8 Expression in Third Trimester Decidual Cells

2006 ◽  
Vol 169 (4) ◽  
pp. 1294-1302 ◽  
Author(s):  
Charles J. Lockwood ◽  
Felice Arcuri ◽  
Paolo Toti ◽  
Claudio De Felice ◽  
Graciela Krikun ◽  
...  
Keyword(s):  
Tumor Necrosis Factor ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 8 ◽  
Interleukin 1Β ◽  
Third Trimester ◽  
Decidual Cells ◽  
Factor Α ◽  
Necrosis Factor

Stability of Tumor Necrosis Factor α, Interleukin 6, and Interleukin 8 in Blood Samples of Patients With Systemic Immune Activation

2008 ◽  
Vol 132 (11) ◽  
pp. 1802-1806 ◽  
Author(s):  
Astrid Friebe ◽  
Hans-Dieter Volk
Keyword(s):  
Tumor Necrosis Factor ◽  
Immune Activation ◽  
Interleukin 6 ◽  
Tumor Necrosis ◽  
Tumor Necrosis Factor Α ◽  
Interleukin 8 ◽  
Test Results ◽  
Systemic Immune Activation ◽  
Factor Α ◽  
Necrosis Factor

Abstract Context.—Tumor necrosis factor α, interleukin 6, and interleukin 8 serum/plasma levels are frequently used for the monitoring of patients with systemic immune activation/ sepsis. This requires comparability of test results over time. However, cytokines are usually not considered to be very stable after blood collection, which might artificially interfere with test results. Objective.—To obtain better knowledge about stability of these cytokines in blood samples for interpretation of test results. Design.—Blood of patients with systemic immune activation was collected in EDTA, lithium heparin, ammonium heparin, and serum tubes. Aliquots were analyzed after storage at room temperature for 2 to 8 hours. Additionally, storage conditions for separated serum/plasma for 24 hours and the reproducibility of repeated cytokine measurements by an automated DPC Immulite analyzer were tested. Results.—Tumor necrosis factor α level was stable in EDTA plasma for 8 hours, while slightly increasing in heparin plasma and serum. Interleukin 6 concentrations were stable for 8 hours in all blood types, whereas interleukin 8 concentrations were stable only in EDTA plasma and were strongly increasing in heparin plasma and serum. Cytokine concentrations in separated serum/plasma were stable during 24 hours if stored at 4°C or frozen at −20 or −70°C. Reproducibility of repeated cytokine measurements revealed no significant differences for all blood types. Conclusions.—Cytokine levels were most critically influenced by the period between blood collection and plasma separation, but its impact was strongly dependent on cytokine and anticoagulant. However, under appropriate conditions cytokine levels were surprisingly stable for up to 8 hours.

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