scholarly journals Commentary: The reliability of telomere length measurements

2015 ◽  
Vol 44 (5) ◽  
pp. 1683-1686 ◽  
Author(s):  
Simon Verhulst ◽  
Ezra Susser ◽  
Pam R Factor-Litvak ◽  
Mirre JP Simons ◽  
Athanase Benetos ◽  
...  
Keyword(s):  
Telomere Length ◽  
Length Measurements

scholarly journals Response to: Reliability and validity of telomere length measurements

2016 ◽  
Vol 45 (4) ◽  
pp. 1298-1301 ◽  
Author(s):  
Simon Verhulst ◽  
Ezra Susser ◽  
Pam R Factor-Litvak ◽  
Mirre Simons ◽  
Athanase Benetos ◽  
...  
Keyword(s):  
Telomere Length ◽  
Reliability And Validity ◽  
Length Measurements

scholarly journals Influence of DNA extraction methods on relative telomere length measurements and its impact on epidemiological studies

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Julia Raschenberger ◽  
Claudia Lamina ◽  
Margot Haun ◽  
Barbara Kollerits ◽  
Stefan Coassin ◽  
...  
Keyword(s):  
Telomere Length ◽  
Dna Extraction ◽  
Extraction Methods ◽  
Epidemiological Studies ◽  
Relative Telomere Length ◽  
Length Measurements ◽  
Dna Extraction Methods

scholarly journals Utility of telomere length measurements for age determination of humpback whales

2014 ◽  
Vol 8 ◽  
Author(s):  
Morten Tange Olsen ◽  
Jooke Robbins ◽  
Martine Bérubé ◽  
Mary Beth Rew ◽  
Per J Palsbøll
Keyword(s):  
Telomere Length ◽  
Quantitative Pcr ◽  
North Atlantic ◽  
Age Determination ◽  
Humpback Whales ◽  
Biological Variability ◽  
Trade Offs ◽  
Length Measurements ◽  
Free Ranging

This study examines the applicability of telomere length measurements by quantitative PCR as a tool for minimally invasive age determination of free-ranging cetaceans. We analysed telomere length in skin samples from 28 North Atlantic humpback whales (Megaptera novaeangliae), ranging from 0 to 26 years of age. The results suggested a significant correlation between telomere length and age in humpback whales. However, telomere length was highly variable among individuals of similar age, suggesting that telomere length measured by quantitative PCR is an imprecise determinant of age in humpback whales. The observed variation in individual telomere length was found to be a function of both experimental and biological variability, with the latter perhaps reflecting patterns of inheritance, resource allocation trade-offs, and stochasticity of the marine environment.

scholarly journals Universal assay for measuring vertebrate telomeres by real-time quantitative PCR

2019 ◽  
Author(s):  
Stephanie F Hudon ◽  
Esteban Palencia Hurtado ◽  
James D. Beck ◽  
Steven J. Burden ◽  
Devin P. Bendixsen ◽  
...  
Keyword(s):  
Telomere Length ◽  
Real Time ◽  
Quantitative Pcr ◽  
Model Organism ◽  
Dna Repeats ◽  
Vertebrate Species ◽  
Computational Tool ◽  
Telomeric Dna ◽  
Real Time Quantitative Pcr ◽  
Length Measurements

Telomere length dynamics are an established biomarker of health and aging in animals. The study of telomeres in numerous species has been facilitated by methods to measure telomere length by real-time quantitative PCR (qPCR). In this method, telomere length is determined by quantifying the amount of telomeric DNA repeats in a sample and normalizing this to the total amount of genomic DNA. This normalization requires the development of genomic reference primers suitable for qPCR, which remains challenging in non-model organism with genomes that have not been sequenced. Here we report reference primers that can be used in qPCR to measure telomere lengths in any vertebrate species. We designed primer pairs to amplify genetic elements that are highly conserved between evolutionarily distant taxa and tested them in species that span the vertebrate tree of life. We report five primer pairs that meet the specificity and reproducibility standards of qPCR. In addition, we demonstrate how to choose the best primers for a given species by testing the primers on multiple individuals within a species and applying an established computational tool. These reference primers can facilitate the application of qPCR-based telomere length measurements in any vertebrate species of ecological or economic interest.

scholarly journals Telomere length measurements using digital fluorescence microscopy

Cytometry ◽  
1999 ◽  
Vol 36 (4) ◽  
pp. 267-278 ◽  
Author(s):  
Steven S.S. Poon ◽  
Uwe M. Martens ◽  
Rabab K. Ward ◽  
Peter M. Lansdorp
Keyword(s):  
Fluorescence Microscopy ◽  
Telomere Length ◽  
Length Measurements ◽  
Digital Fluorescence Microscopy

TELOMERE LENGTH MEASUREMENTS IN HEMATOPOIETIC CELLS USING IN SITU HYBRIDIZATION TECHNIQUES

2000 ◽  
Vol 28 (1) ◽  
pp. A3-A3
Author(s):  
U. M. Martens ◽  
V. Brass ◽  
C.F. Waller ◽  
W. Lange ◽  
S.S.S. Poon ◽  
...  
Keyword(s):  
In Situ Hybridization ◽  
Telomere Length ◽  
Hematopoietic Cells ◽  
Length Measurements

scholarly journals Method Specific Calibration Corrects for DNA Extraction Method Effects on Relative Telomere Length Measurements by Quantitative PCR

PLoS ONE ◽  
2016 ◽  
Vol 11 (10) ◽  
pp. e0164046 ◽  
Author(s):  
Luise A. Seeker ◽  
Rebecca Holland ◽  
Sarah Underwood ◽  
Jennifer Fairlie ◽  
Androniki Psifidi ◽  
...  
Keyword(s):  
Telomere Length ◽  
Dna Extraction ◽  
Quantitative Pcr ◽  
Extraction Method ◽  
Relative Telomere Length ◽  
Dna Extraction Method ◽  
Length Measurements ◽  
Method Effects

scholarly journals TERC and TERT gene mutations in patients with bone marrow failure and the significance of telomere length measurements

Blood ◽  
2009 ◽  
Vol 113 (2) ◽  
pp. 309-316 ◽  
Author(s):  
Hong-Yan Du ◽  
Elena Pumbo ◽  
Jennifer Ivanovich ◽  
Ping An ◽  
Richard T. Maziarz ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Telomere Length ◽  
Mononuclear Cells ◽  
Medical Center ◽  
Bone Marrow Failure ◽  
Screening Method ◽  
Gene Mutations ◽  
Telomere Shortening ◽  
Tert Gene ◽  
Length Measurements

Abstract Dyskeratosis congenita (DC) is a rare inherited form of bone marrow failure (BMF) caused by mutations in telomere maintaining genes including TERC and TERT. Here we studied the prevalence of TERC and TERT gene mutations and of telomere shortening in an unselected population of patients with BMF at our medical center and in a selected group of patients referred from outside institutions. Less than 5% of patients with BMF had pathogenic mutations in TERC or TERT. In patients with BMF, pathogenic TERC or TERT gene mutations were invariably associated with marked telomere shortening (≪ 1st percentile) in peripheral blood mononuclear cells (PBMCs). In asymptomatic family members, however, telomere length was not a reliable predictor for the presence or absence of a TERC or TERT gene mutation. Telomere shortening was not pathognomonic of DC, as approximately 30% of patients with BMF due to other causes had PBMC telomere lengths at the 1st percentile or lower. We conclude that in the setting of BMF, measurement of telomere length is a sensitive but nonspecific screening method for DC. In the absence of BMF, telomere length measurements should be interpreted with caution.

scholarly journals Telomere attrition with age in a wild amphibian population

2020 ◽  
Vol 16 (7) ◽  
pp. 20200168
Author(s):  
Gregorio Sánchez-Montes ◽  
Íñigo Martínez-Solano ◽  
Carmen Díaz-Paniagua ◽  
Antonio Vilches ◽  
Arturo H. Ariño ◽  
...  
Keyword(s):  
Telomere Length ◽  
Breeding Population ◽  
Length Variation ◽  
Age Classes ◽  
Cross Sectional ◽  
Telomere Shortening ◽  
Amphibian Population ◽  
Telomere Attrition ◽  
Length Measurements ◽  
Epidalea Calamita

Telomere shortening with age has been documented in many organisms, but few studies have reported telomere length measurements in amphibians, and no information is available for growth after metamorphosis, nor in wild populations. We provide both cross-sectional and longitudinal evidence of net telomere attrition with age in a wild amphibian population of natterjack toads ( Epidalea calamita ). Based on age-estimation by skeletochronology and qPCR telomere length measurements in the framework of an individual-based monitoring programme, we confirmed telomere attrition in recaptured males. Our results support that toads experience telomere attrition throughout their ontogeny, and that most attrition occurs during the first 1–2 years. We did not find associations between telomere length and inbreeding or body condition. Our results on telomere length dynamics under natural conditions confirm telomere shortening with age in amphibians and provide quantification of wide telomere length variation within and among age-classes in a wild breeding population.

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