scholarly journals Adipose tissue-derived stem cells in a fibrin implant enhance neovascularization in a peritoneal grafting site: a potential way to improve ovarian tissue transplantation

2018 ◽  
Vol 33 (2) ◽  
pp. 270-279 ◽  
Author(s):  
D D Manavella ◽  
L Cacciottola ◽  
C M Desmet ◽  
B F Jordan ◽  
J Donnez ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Ovarian Tissue ◽  
Tissue Transplantation ◽  
Ovarian Tissue Transplantation

scholarly journals Long-Term Advantages of Ovarian Reserve Maintenance and Follicle Development Using Adipose Tissue-Derived Stem Cells in Ovarian Tissue Transplantation

2020 ◽  
Vol 9 (9) ◽  
pp. 2980 ◽  
Author(s):  
Luciana Cacciottola ◽  
Thu Y. T. Nguyen ◽  
Maria C. Chiti ◽  
Alessandra Camboni ◽  
Christiani A. Amorim ◽  
...  
Keyword(s):  
Stem Cells ◽  
Adipose Tissue ◽  
Ovarian Tissue ◽  
Short Interval ◽  
Primordial Follicle ◽  
Tissue Transplantation ◽  
Follicle Development ◽  
Long Term Effects ◽  
Ovarian Tissue Transplantation

(1) Background: Ovarian tissue transplantation with adipose tissue-derived stem cells (ASCs) has been shown to enhance graft vascularization and increase follicle survival after a short interval of 7 days. The aim of the present study was to investigate their long-term effects on primordial follicle pool maintenance and follicle development. (2) Methods: A total of 14 severe combined immunodeficient (SCID) mice were grafted with frozen-thawed human ovarian tissue with or without ASCs. Blood was taken monthly in order to quantify the anti-Müllerian hormone (AMH) and estradiol. After 6 months, all the grafts were retrieved and sent for histology and immunolabeling (AMH, AMH receptor II, estrogen receptors α and β, and c-kit/kit ligand). (3) Results: A significant upturn was observed in AMH and estradiol plasma levels 4 months after transplantation in both grafted groups. The primordial follicle pool was better preserved in the ASC group (41.86 ± 28.35) than in the standard transplantation group (9.65 ± 17.6, p < 0.05) compared to non-grafted controls (124.7 ± 140). (4) Conclusions: The use of ASCs prior to ovarian tissue transplantation yielded a larger primordial follicle pool and more physiological follicle distribution after long-term grafting. These findings suggested that ASC use might extend the ovarian tissue lifespan.

Ovarian tissue transplantation

2011 ◽  
pp. 357-366 ◽  
Author(s):  
Jacques Donnez ◽  
Jean Squifflet ◽  
Marie-Madeleine Dolmans
Keyword(s):  
Ovarian Tissue ◽  
Tissue Transplantation ◽  
Ovarian Tissue Transplantation

Livebirth after cryopreserved ovarian tissue transplantation – Authors' reply

The Lancet ◽  
2012 ◽  
Vol 380 (9837) ◽  
pp. 107
Author(s):  
J Donnez ◽  
MM Dolmans ◽  
D Demylle ◽  
P Jadoul ◽  
C Pirard ◽  
...  
Keyword(s):  
Ovarian Tissue ◽  
Tissue Transplantation ◽  
Ovarian Tissue Transplantation ◽  
Cryopreserved Ovarian Tissue

scholarly journals Is Ovarian Tissue Transplantation Safe in Patients with Central Nervous System Primitive Neuroectodermal Tumors?

2020 ◽  
Vol 9 (12) ◽  
pp. 4101
Author(s):  
Thu Yen Thi Nguyen ◽  
Alessandra Camboni ◽  
Rossella Masciangelo ◽  
Jacques Donnez ◽  
Marie-Madeleine Dolmans
Keyword(s):  
Cancer Cells ◽  
Ovarian Tissue ◽  
Neuron Specific Enolase ◽  
Cancer Recurrence ◽  
Tissue Transplantation ◽  
Ovarian Tissue Transplantation ◽  
Primitive Neuroectodermal Tumors ◽  
Immunodeficient Mice ◽  
Neuroectodermal Tumors ◽  
Digital Polymerase Chain Reaction

The risk of reseeding malignancy harbored in cryopreserved and transplanted ovarian tissue has been a source of concern. This study aimed to determine the potential relationship between frozen–thawed ovarian tissue transplantation and primary cancer recurrence. Three patients with cerebral primitive neuroectodermal tumors (PNET) were included in this study. One woman gave birth to three healthy babies following reimplantation of her cryopreserved ovarian tissue, but subsequently died due to cancer relapse six years after ovarian tissue transplantation. The second subject died from progressive cancer, while the third is still alive and awaiting reimplantation of her ovarian tissue in due course. Frozen ovarian cortex from all three patients was analyzed and xenotransplanted to immunodeficient mice for five months. Main outcomes were the presence of cancer cells in the thawed and xenografted ovarian tissue at histology, immunostaining (expression of neuron-specific enolase and glial fibrillary acidic protein (GFAP)), and reverse-transcription droplet digital polymerase chain reaction (RT-ddPCR) (levels of enolase 2 and GFAP). In conclusion, no malignant cells were detected in ovarian tissue from patients with PNET, even in those who experienced recurrence of the disease, meaning that the risk of reseeding cancer cells with ovarian tissue transplantation in these patients can be considered low.

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