scholarly journals Frozen–thawed embryo transfers in natural cycles with spontaneous or induced ovulation: the search for the best protocol continues

2016 ◽  
Vol 31 (12) ◽  
pp. 2803-2810 ◽  
Author(s):  
M. Montagut ◽  
S. Santos-Ribeiro ◽  
M. De Vos ◽  
N.P. Polyzos ◽  
P. Drakopoulos ◽  
...  
Reproduction ◽  
2001 ◽  
pp. 777-783 ◽  
Author(s):  
DE Hickford ◽  
NE Merry ◽  
MH Johnson ◽  
L Selwood

Induced ovulation resulting in normal embryos is rare in marsupials. In this study natural and induced ovulations were compared in mature Sminthopsis macroura (n = 122). Comparison of maturation of preovulatory oocytes by ovarian histology and examination of oocytes removed from developing follicles in 12 ovaries of 23 animals receiving 0.058 iu equine serum gonadotrophin (eSG) g(-1) with ovaries of 12 animals undergoing natural cycles showed that oocyte maturation was significantly more irregular when it was induced (P < 0.001). Postovulatory stages were examined by estimating the number of eggs ovulated from ovarian histology, and by counting oviduct and uterine contents recovered after ovulation. S. macroura receiving 0.087 iu eSG g(-1) (n = 34), administered as one (n = 17) or two (n = 17) injections, were significantly (P < 0.05) more likely to ovulate (74%), mate (80%) and have conceptuses (66%) than were animals receiving 0.058 iu eSG g(-1) (12, 53 and 0%, respectively) (n = 17), and the values were similar to those in animals (n = 36) undergoing natural cycles (100, 81 and 56%, respectively). Induced ovulation using 0.087 iu eSG g(-1) yielded significantly (P < 0.05) more oocytes per ovary (20.8 +/- 8.5; combined data) than did ovulation in animals undergoing natural cycles (13.7 +/- 3.2) (ANOVA, t test). The responses of animals induced in different phases of the oestrous cycle with 0.087 iu eSG g(-1) were not significantly different (ANOVA) with respect to the number of corpora lutea per ovary, conceptuses per animal or days to ovulation after injection. However, the proportion of females that responded after receiving 0.058 iu eSG g(-1) in the luteal phase was significantly different from that in animals treated with the same dose in the intermediate phase (P < 0.01) and in non-cyclic females treated with 0.058 iu eSG g(-1) (P < 0.02). The main benefits of the treatment were that normal embryos resulted and that 70-78% of non-cyclic animals could be induced to ovulate.


Reproduction ◽  
2010 ◽  
Vol 139 (2) ◽  
pp. 419-425 ◽  
Author(s):  
Phil Chi Khang Au ◽  
Angela Nation ◽  
Marissa Parrott ◽  
Lynne Selwood

Induced ovulation maximizes captive breeding success, increasing productivity and facilitating the contribution of otherwise infertile animals to the genetic pool. In marsupials, induced ovulation to produce fertile young is unknown. Here we present an induction protocol efficient in inducing non-cycling and non-reproductive females to cycle, mate, ovulate, and conceive. Ovulation was induced in Sminthopsis macroura using an initial injection of 0.06 IU equine serum gonadotropin (eSG)/g (time 0), followed on day 4 by 0.04 IU eSG/g. Using this induction regime, the timing of follicular and embryonic development mimics natural cycles and results in the birth of viable, fertile young. Response to induction is not significantly affected by animal age, making this protocol an effective conservation tool. We have established a time-table of development following induction, providing a source of precisely timed research material. This is the first induced ovulation protocol in any marsupial to result in demonstrated fertile offspring and to allow the reliable collection of known-age samples during both the follicular phase and the gestation period.


Aquaculture ◽  
1981 ◽  
Vol 26 (1-2) ◽  
pp. 117-127 ◽  
Author(s):  
George A. Hunter ◽  
Edward M. Donaldson ◽  
Helen M. Dye

2010 ◽  
Vol 94 (4) ◽  
pp. S166
Author(s):  
E. Chang ◽  
M. Kim ◽  
J. Han ◽  
H. Won ◽  
W. Lee ◽  
...  
Keyword(s):  

2001 ◽  
Vol 16 (12) ◽  
pp. 2501-2507 ◽  
Author(s):  
Misao Fukuda ◽  
Kiyomi Fukuda ◽  
Claus Yding Andersen ◽  
Anne Grete Byskov
Keyword(s):  

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