scholarly journals Proteomic characterization of macro-, micro- and nano-extracellular vesicles derived from the same first trimester placenta: relevance for feto-maternal communication

2016 ◽  
Vol 31 (4) ◽  
pp. 687-699 ◽  
Author(s):  
Mancy Tong ◽  
Torsten Kleffmann ◽  
Shantanu Pradhan ◽  
Caroline L. Johansson ◽  
Joana DeSousa ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
First Trimester ◽  
Maternal Communication

scholarly journals 109 Isolation and characterization of myometrium and decidual cell-derived extracellular vesicles

2021 ◽  
Vol 224 (2) ◽  
pp. S75-S76
Author(s):  
Megan Shepherd ◽  
Enkhtuya Radnaa ◽  
Rheanna Urrabaz-Garza ◽  
Talar Kechichian ◽  
Ourlad Alzeus G. Tantengco ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Decidual Cell ◽  
Isolation And Characterization

scholarly journals Emerging techniques in the isolation and characterization of extracellular vesicles and their roles in cancer diagnostics and prognostics

The Analyst ◽  
2016 ◽  
Vol 141 (2) ◽  
pp. 371-381 ◽  
Author(s):  
Vijaya Sunkara ◽  
Hyun-Kyung Woo ◽  
Yoon-Kyoung Cho
Keyword(s):  
Extracellular Vesicles ◽  
Cancer Diagnostics ◽  
Clinical Implementation ◽  
Characterization Methods ◽  
Cancer Management ◽  
Isolation And Characterization

We present an overview of current isolation, detection, and characterization methods of extracellular vesicles and their applications and limitations as a potential emerging biomarker in cancer management and their clinical implementation.

scholarly journals Characterization of alkaline phosphatases from human first trimester placentas.

1979 ◽  
Vol 254 (3) ◽  
pp. 935-938 ◽  
Author(s):  
T. Sakiyama ◽  
J.C. Robinson ◽  
J.Y. Chou
Keyword(s):  
First Trimester ◽  
Alkaline Phosphatases

Isolation and Characterization of Extracellular Vesicles in Stem Cell-Related Studies

2017 ◽  
pp. 205-223
Author(s):  
Zezhou Zhao ◽  
Dillon C. Muth ◽  
Vasiliki Mahairaki ◽  
Linzhao Cheng ◽  
Kenneth W. Witwer
Keyword(s):  
Stem Cell ◽  
Extracellular Vesicles ◽  
Isolation And Characterization

scholarly journals Label-Free Prostate Cancer Detection by Characterization of Extracellular Vesicles Using Raman Spectroscopy

2018 ◽  
Vol 90 (19) ◽  
pp. 11290-11296 ◽  
Author(s):  
Wooje Lee ◽  
Afroditi Nanou ◽  
Linda Rikkert ◽  
Frank A. W. Coumans ◽  
Cees Otto ◽  
...  
Keyword(s):  
Prostate Cancer ◽  
Raman Spectroscopy ◽  
Extracellular Vesicles ◽  
Cancer Detection ◽  
Label Free ◽  
Prostate Cancer Detection

The more the better – determining the optimal range when performing single-vesicle phenotyping

2021 ◽  
Vol 1 (1) ◽  
pp. 26-33
Author(s):  
María Gómez-Serrano ◽  
Christian Preußer ◽  
Kathrin Stelter ◽  
Elke Pogge von Strandmann
Keyword(s):  
Extracellular Vesicles ◽  
Particle Number ◽  
Experiment Design ◽  
Surface Markers ◽  
Optimal Range ◽  
Design And Optimization ◽  
Highly Sensitive ◽  
Single Vesicle

The characterization of extracellular vesicles (EVs) has evolved rapidly in recent years due to advances in straightforward technologies. Based on these more sensitive methods, it is now possible to describe EV populations in their entirety more precisely. However, these applications require an equivalently delicate experiment design and optimization steps to draw valid conclusions in the end. One of these methods is represented by the highly sensitive nanoflow cytometry (nFCM), by which particles can be analyzed not only on their size (< 40 nm) and concentration but also concerning surface markers. In this work, we addressed some of the potential caveats of this method, especially when characterizing particles with fluorescently labelled antibodies. In particular, we show, when using low particle concentrations, which are inevitably encountered when working with EVs, the characterization of surface markers is prone to significantly varying. We hypothesized that these technical limitations could respond to the stickiness of EVs and should be properly counteracted. As a reference, we strongly recommend performing particle number-based comparisons with at least 109 particles as staining input in nFCM analyses. Moreover, we provided representative particle-number based immunoblotting results, underlying the significance of this parameter as a normalizer in future EV research.

Zika virus hijacks extracellular vesicle tetraspanin pathways for cell-to-cell transmission

2021 ◽  
Author(s):  
Sara B. York ◽  
Li Sun ◽  
Allaura S. Cone ◽  
Leanne C. Duke ◽  
Mujeeb R. Cheerathodi ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Intercellular Communication ◽  
Zika Virus ◽  
Biological Fluids ◽  
Virus Transmission ◽  
First Trimester ◽  
Enhanced Production ◽  
Infected Cells ◽  
Encapsulated Structures

ABSTRACTExtracellular vesicles (EVs) are membrane-encapsulated structures released by cells which carry signaling factors, proteins and microRNAs that mediate intercellular communication. Accumulating evidence supports an important role of EVs in the progression of neurological conditions and both the spread and pathogenesis of infectious diseases. It has recently been demonstrated that EVs from Hepatitis C virus (HCV) infected individuals and cells contained replicative-competent viral RNA that was capable of infecting hepatocytes. Being a member of the same viral family, it is likely the Zika virus also hijacks EV pathways to package viral components and secrete vesicles that are infectious and potentially less immunogenic. As EVs have been shown to cross blood-brain and placental barriers, it is possible that Zika virus could usurp normal EV biology to gain access to the brain or developing fetus. Here, we demonstrate that Zika virus infected cells secrete distinct EV sub-populations with specific viral protein profiles and infectious genomes. Zika virus infection resulted in the enhanced production of EVs with varying sizes and density compared to those released from non-infected cells. We also show that the EV enriched tetraspanin CD63 regulates the release of EVs, and Zika viral genomes and capsids following infection. Overall, these findings provide evidence for an alternative means of Zika virus transmission and demonstrate the role of EV biogenesis and trafficking proteins in the modulation of Zika infection.ImportanceZika virus is a re-emerging infectious disease that spread rapidly across the Caribbean and South America. Infection of pregnant women during the first trimester has been linked to microcephaly, a neurological condition where babies are born with smaller heads due to abnormal brain development. Babies born with microcephaly can develop convulsions and suffer disabilities as they age. Despite the significance of Zika virus, little is known about how the virus infects the fetus or causes disease. Extracellular vesicles (EVs) are membrane-encapsulated structures released by cells that are present in all biological fluids. EVs carry signaling factors, proteins and microRNAs that mediate intercellular communication. EVs have been shown to be a means by which some viruses can alter cellular environments and cross previously unpassable cellular barriers. Thus gaining a greater understanding of how Zika affects EV cargo may aid in the development of better diagnostics, targeted therapeutics and prophylactic treatments.

Detection and characterization of extracellular vesicles in exhaled breath condensate and sputum of COPD and severe asthma patients

2021 ◽  
pp. 2003024
Author(s):  
Donatella Lucchetti ◽  
Giuseppe Santini ◽  
Luigi Perelli ◽  
Claudio Ricciardi-Tenore ◽  
Filomena Colella ◽  
...  
Keyword(s):  
Severe Asthma ◽  
Extracellular Vesicles ◽  
Exhaled Breath Condensate ◽  
Exhaled Breath ◽  
Asthma Patients ◽  
Breath Condensate

scholarly journals Proteomic, transcriptomic and functional characterization of human astrocyte‐derived extracellular vesicles upon inflammatory activation

2020 ◽  
Vol 16 (S2) ◽  
Author(s):  
Yang You ◽  
Kathleen Borgmann ◽  
VenkataViswa Edara ◽  
Satomi Stacy ◽  
Anuja Ghorpade ◽  
...  
Keyword(s):  
Extracellular Vesicles ◽  
Functional Characterization ◽  
Human Astrocyte ◽  
Inflammatory Activation
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