Developmental kinetics of cleavage stage mouse embryos are related to their subsequent carbohydrate and amino acid utilization at the blastocyst stage

2015 ◽  
Vol 30 (3) ◽  
pp. 543-552 ◽  
Author(s):  
Y. S. L. Lee ◽  
G. A. Thouas ◽  
D. K. Gardner
Keyword(s):  
Amino Acid ◽  
Blastocyst Stage ◽  
Mouse Embryos ◽  
Cleavage Stage ◽  
Amino Acid Utilization ◽  
Kinetics Of

174. ATMOSPHERIC OXYGEN ALTERS THE EMBRYONIC METABOLOME AS QUANTIFIED BY CARBOHYDRATE UPTAKE AND AMINO ACID UTILISATION

2010 ◽  
Vol 22 (9) ◽  
pp. 92
Author(s):  
P. L. Wale ◽  
D. K. Gardner
Keyword(s):  
Amino Acid ◽  
Atmospheric Oxygen ◽  
Oxygen Toxicity ◽  
Glucose Consumption ◽  
Blastocyst Stage ◽  
Quadrupole Mass ◽  
F1 Hybrid ◽  
Amino Acid Utilization ◽  
Hybrid Mice ◽  
Amino Acid Utilisation

Oxygen regulates embryo development at both the cleavage and post compaction stages. In this study we investigated the effects of atmospheric oxygen during the preimplantation stages, on the post-compaction embryonic metabolome through quantification of glucose consumption and amino acid utilization. Zygotes obtained from F1 hybrid mice (C57BLxCBA) were randomly allocated to either 5 or 20% oxygen. In the first experiment, following the first 48 h of culture, embryos were cultured individually in 1 μL drops of modified G2 medium (0.5 mM glucose) and moved to fresh drops of medium every 24 h. The glucose concentration in the spent media samples, including controls containing no embryo, was determined by microfluorimetry. In the second experiment, embryos which had developed to the early blastocyst stage after 72 h were cultured for a further 24 h in groups of 10 in 2 μL drops of G2. Analysis of amino acid utilization was performed using liquid chromatography-triple quadrupole mass spectrometry. Glucose consumption by embryos cultured in 5% oxygen was significantly greater on day 4 and day 5 (4.89 ± 0.29 and 6.13 ± 0.41 pmol/embryo/h) compared to embryos cultured in 20% oxygen (2.59 ± 0.40 and 5.09 ± 0.28 pmol/embryo/h; P < 0.05). In contrast amino acid utilisation by embryos cultured in 5% oxygen was significantly less than embryos cultured in 20% oxygen (P < 0.05). The data generated will help to determine the aetiology of oxygen toxicity to the preimplantation embryo. Higher glucose utilisation by embryos in 5% oxygen is consistent with their improved development. Conversely, the increased utilisation of amino acids by blastocysts in 20% oxygen may reflect an adaptation to increased oxidative stress as a result of culture in a non-physiological oxygen concentration. This study demonstrates that atmospheric oxygen during the preimplantation period perturbs the embryonic metabolome which results in a compensatory increase in amino acid utilisation.

scholarly journals METABOLIC CONNECTION BETWEEN PROLINE AND GLYCINE IN THE AMINO ACID UTILIZATION OF TORULOPSIS UTILIS

1947 ◽  
Vol 169 (3) ◽  
pp. 759-760
Author(s):  
G. Ehrensvärd ◽  
E. Sperber ◽  
E. Saluste ◽  
L. Reio ◽  
R. Stjernholm
Keyword(s):  
Amino Acid ◽  
Amino Acid Utilization

scholarly journals Comparing the cumulative live birth rate of cleavage-stage versus blastocyst-stage embryo transfers between IVF cycles: a study protocol for a multicentre randomised controlled superiority trial (the ToF trial)

BMJ Open ◽  
2021 ◽  
Vol 11 (1) ◽  
pp. e042395
Author(s):  
Simone Cornelisse ◽  
Liliana Ramos ◽  
Brigitte Arends ◽  
Janneke J Brink-van der Vlugt ◽  
Jan Peter de Bruin ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Live Birth ◽  
Birth Rate ◽  
Live Birth Rate ◽  
Oocyte Retrieval ◽  
Blastocyst Stage ◽  
Cleavage Stage ◽  
Cumulative Live Birth Rate ◽  
Cumulative Live Birth ◽  
Superiority Trial

IntroductionIn vitro fertilisation (IVF) has evolved as an intervention of choice to help couples with infertility to conceive. In the last decade, a strategy change in the day of embryo transfer has been developed. Many IVF centres choose nowadays to transfer at later stages of embryo development, for example, transferring embryos at blastocyst stage instead of cleavage stage. However, it still is not known which embryo transfer policy in IVF is more efficient in terms of cumulative live birth rate (cLBR), following a fresh and the subsequent frozen–thawed transfers after one oocyte retrieval. Furthermore, studies reporting on obstetric and neonatal outcomes from both transfer policies are limited.Methods and analysisWe have set up a multicentre randomised superiority trial in the Netherlands, named the Three or Fivetrial. We plan to include 1200 women with an indication for IVF with at least four embryos available on day 2 after the oocyte retrieval. Women are randomly allocated to either (1) control group: embryo transfer on day 3 and cryopreservation of supernumerary good-quality embryos on day 3 or 4, or (2) intervention group: embryo transfer on day 5 and cryopreservation of supernumerary good-quality embryos on day 5 or 6. The primary outcome is the cLBR per oocyte retrieval. Secondary outcomes include LBR following fresh transfer, multiple pregnancy rate and time until pregnancy leading a live birth. We will also assess the obstetric and neonatal outcomes, costs and patients’ treatment burden.Ethics and disseminationThe study protocol has been approved by the Central Committee on Research involving Human Subjects in the Netherlands in June 2018 (CCMO NL 64060.000.18). The results of this trial will be submitted for publication in international peer-reviewed and in open access journals.Trial registration numberNetherlands Trial Register (NL 6857).

scholarly journals Kinetics of Mixed Amino Acid and Ionic Liquid on CO2 Hydrate Formation

2019 ◽  
Vol 495 ◽  
pp. 012073 ◽  
Author(s):  
Cornelius B. Bavoh ◽  
Bhajan Lal ◽  
Joel Ben-Awuah ◽  
Muhammad Saad Khan ◽  
Grace Ofori-Sarpong
Keyword(s):  
Ionic Liquid ◽  
Amino Acid ◽  
Hydrate Formation ◽  
Kinetics Of

Micellar Catalytic Effects on the Kinetics of the Ionization of Basic Amino Acid and Acidic Amino Acid Studied by the Ultrasonic Absorption Method

Langmuir ◽  
1995 ◽  
Vol 11 (5) ◽  
pp. 1477-1481
Author(s):  
Teruyo Yamashita ◽  
Miyuki Yamasaki ◽  
Takayuki Sano ◽  
Shoji Harada ◽  
Hiroshige Yano
Keyword(s):  
Amino Acid ◽  
Basic Amino Acid ◽  
Ultrasonic Absorption ◽  
Absorption Method ◽  
Acidic Amino Acid ◽  
Kinetics Of ◽  
Catalytic Effects

SOME ASPECTS OF THE STRUCTURE OF HEMOGLOBIN

1964 ◽  
Vol 42 (6) ◽  
pp. 755-762 ◽  
Author(s):  
David B. Smith
Keyword(s):  
Amino Acids ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Partial Amino Acid Sequence ◽  
Heme Pocket ◽  
Terminal Amino ◽  
Polypeptide Chains ◽  
Kinetics Of ◽  
Β Chain

An outline of present ideas concerning the arrangement, folding, and chemistry of the polypeptide chains of hemoglobin is given with some references to present know ledge of myoglobin.New material includes a partial amino acid sequence of the β-chain of horse hemoglobin, details concerning the amino acids lining the heme pocket of horse hemoglobin, and the effects of carboxypeptidases A and B on horse oxy- and horse deoxy-hemoglobin. The kinetics of the latter reactions are not simple. The C-terminal amino acids are released more rapidly from the oxygenated form.

scholarly journals Identification in skeletal muscle of a distinct extracellular pool of amino acids, and its role in protein synthesis

1971 ◽  
Vol 121 (5) ◽  
pp. 817-827 ◽  
Author(s):  
R. C. Hider ◽  
E. B. Fern ◽  
D. R. London
Keyword(s):  
Skeletal Muscle ◽  
Amino Acids ◽  
Protein Synthesis ◽  
Amino Acid ◽  
Incubation Medium ◽  
Extensor Digitorum Longus ◽  
Extensor Digitorum ◽  
Longus Muscle ◽  
Kinetics Of

1. The kinetics of radioactive labelling of extra- and intra-cellular amino acid pools and protein of the extensor digitorum longus muscle were studied after incubations with radioactive amino acids in vitro. 2. The results indicated that an extracellular pool could be defined, the contents of which were different from those of the incubation medium. 3. It was concluded that amino acids from the extracellular pool, as defined in this study, were incorporated directly into protein.

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