scholarly journals Insulin-like growth factor 1 increases apical fibronectin in blastocysts to increase blastocyst attachment to endometrial epithelial cells in vitro

2014 ◽  
Vol 30 (2) ◽  
pp. 284-298 ◽  
Author(s):  
C. J. Green ◽  
S. T. Fraser ◽  
M. L. Day
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Insulin Like Growth Factor ◽  
Endometrial Epithelial Cells

scholarly journals Growth factor induced proliferation, migration, and lumen formation of rat endometrial epithelial cells in vitro

2016 ◽  
Vol 62 (3) ◽  
pp. 271-278 ◽  
Author(s):  
Md. Rashedul ISLAM ◽  
Kazuki YAMAGAMI ◽  
Yuka YOSHII ◽  
Nobuhiko YAMAUCHI
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Lumen Formation ◽  
Endometrial Epithelial Cells

Effects of epidermal growth factor and transforming growth factor alpha on the mouse trophoblast outgrowth in vitro

1995 ◽  
Vol 133 (6) ◽  
pp. 741-746 ◽  
Author(s):  
Toshifumi Machida ◽  
Michiyoshi Taga ◽  
Hiroshi Minaguchi
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Conditioned Medium ◽  
Transforming Growth Factor ◽  
Transforming Growth Factor Alpha ◽  
Epidermal Growth ◽  
Factor Alpha ◽  
Endometrial Epithelial Cells

Machida T, Taga M, Minaguchi H. Effects of epidermal growth factor and transforming growth factor alpha on the mouse trophoblast outgrowth in vitro. Eur J Endocrinol 1995;133:741–6. ISSN 0804–4643 In order to analyze the involvement of growth factors in the implantation mechanism, we examined the direct effects of epidermal growth factor (EGF) and transforming growth factor alpha (TGF-α) on trophoblast outgrowth of the mouse blastocyst in vitro. ICR mouse blastocysts were cultured for 4 days on a culture plate in medium containing EGF or TGF-α or conditioned medium obtained from cultured endometrial epithelial cells. Blastocysts were also co-cultured with endometrial epithelial cells. The trophoblast outgrowth of these cultured blastocysts was observed daily and the percentage of outgrowing embryos was calculated and analyzed statistically by the chi-squared test. Analysis for the specific binding of 125I-EGF in outgrown trophoblasts was carried out by autoradiography. The coculture (days 3 and 4) and the presence of EGF (10 ng/ml, day 4), TGF-α (1 ng/ml, day 3; 10 ng/ml, days 2 and 3; 50 ng/ml, days 2–4) or conditioned medium (days 3 and 4) significantly stimulated the rate of trophoblast outgrowth. Preincubation of the conditioned medium with monoclonal anti-EGF or anti-TGF-α antibody suppressed the stimulatory effect of the conditioned medium on trophoblast outgrowth. The specific 125I-EGF binding in outgrown trophoblasts was demonstrated by autoradiography. These results suggest that EGF and TGF-α play an important role in the implantation process by directly stimulating trophoblast development. Michiyoshi Taga, Department of Obstetrics and Gynecology, Yokohama City University School of Medicine, 3-9 Fukuura, Kanazawa-ku, Yokohama 236, Japan

scholarly journals Hepatocyte Growth Factor Stimulates Proliferation, Migration, and Lumen Formation of Human Endometrial Epithelial Cells in Vitro

1997 ◽  
Vol 57 (4) ◽  
pp. 936-942 ◽  
Author(s):  
Junichi Sugawara ◽  
Takao Fukaya ◽  
Takashi Murakami ◽  
Hidemune Yoshida ◽  
Akira Yajima
Keyword(s):  
Growth Factor ◽  
Epithelial Cells ◽  
Hepatocyte Growth Factor ◽  
Lumen Formation ◽  
Hepatocyte Growth ◽  
Endometrial Epithelial Cells

scholarly journals Galectin-9: A New Endometrial Epithelial Marker for the Mid- and Late-Secretory and Decidual Phases in Humans

2005 ◽  
Vol 90 (11) ◽  
pp. 6170-6176 ◽  
Author(s):  
Roxana M. Popovici ◽  
Miriam S. Krause ◽  
Ariane Germeyer ◽  
Thomas Strowitzki ◽  
Michael von Wolff
Keyword(s):  
Growth Factor ◽  
Epidermal Growth Factor ◽  
Epithelial Cells ◽  
Rnase Protection Assay ◽  
Interferon Γ ◽  
Human Endometrium ◽  
Rnase Protection ◽  
Epidermal Growth ◽  
Endometrial Epithelial Cells

Abstract Context: The galectin family has been reported to play a role in the regulation of cell growth, cell adhesion, apoptosis, inflammation, and immunomodulation, all of which are important for endometrial function, as well as implantation. Objective: The objective of the study was to investigate the expression and regulation of galectin-9, a β-galactoside-binding lectin in the human endometrium. Design: Galectin-9 mRNA and protein were analyzed in dated endometrial biopsies throughout the menstrual cycle and in human early-pregnancy decidua, as well as in the different endometrial cell compartments. Regulation of galectin-9 by estradiol, progesterone, epidermal growth factor, and interferon-γ in endometrial epithelial cells in vitro was studied. Results: Galectin-9 mRNA analyzed by RNase protection assay is expressed in the human endometrium, specifically in the human endometrial epithelial cells but not in stromal or immune cells. It is expressed at very low concentrations during the proliferative phase and the early-secretory phase and shows a sharp and significant increase in the mid- and late-secretory phases, the window of implantation, as well as in the decidua. Accordingly, galectin-9 protein is also exclusively increased in human endometrial epithelial cells during the mid- and late-secretory phases and in the decidua, however, not in endometrial stromal cells or decidualized cells in vivo or in vitro. A regulation in vitro by estradiol, progesterone, epidermal growth factor, and interferon-γ could not be detected. Conclusions: Based on these findings and on the functional studies of other galectins, we suggest galectin-9 as a novel endometrial marker for the mid- and late-secretory and decidual phases.

Sign in / Sign up

Export Citation Format

Share Document