scholarly journals Dopamine in human follicular fluid is associated with cellular uptake and metabolism-dependent generation of reactive oxygen species in granulosa cells: implications for physiology and pathology

2013 ◽  
Vol 29 (3) ◽  
pp. 555-567 ◽  
Author(s):  
S. Saller ◽  
L. Kunz ◽  
D. Berg ◽  
U. Berg ◽  
H. Lara ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Cellular Uptake ◽  
Granulosa Cells ◽  
Follicular Fluid ◽  
Reactive Oxygen ◽  
Oxygen Species ◽  
Human Follicular Fluid ◽  
Uptake And Metabolism

scholarly journals Norepinephrine, Active Norepinephrine Transporter, and Norepinephrine-Metabolism Are Involved in the Generation of Reactive Oxygen Species in Human Ovarian Granulosa Cells

Endocrinology ◽  
2012 ◽  
Vol 153 (3) ◽  
pp. 1472-1483 ◽  
Author(s):  
S. Saller ◽  
J. Merz-Lange ◽  
S. Raffael ◽  
S. Hecht ◽  
R. Pavlik ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Monoamine Oxidase ◽  
Cellular Uptake ◽  
Granulosa Cells ◽  
Reactive Oxygen ◽  
Monoamine Oxidase A ◽  
Oxygen Species ◽  
Uptake And Metabolism

The neurotransmitter norepinephrine (NE) is derived from the sympathetic nervous system and may be involved in the regulation of ovarian functions. Ovarian innervation increases in patients with polycystic ovarian syndrome (PCOS), prompting us to readdress a role of NE in the human ovary. In vitro fertilization-derived granulosa cells (GC), follicular fluids (FF), and ovarian sections were studied. NE was found in FF and freshly isolated GC, yet significantly lower levels of NE were detected in samples from PCOS patients. Furthermore, the metabolite normetanephrine was detected in FF. Together this suggests cellular uptake and metabolism of NE in GC. In accordance, the NE transporter and NE-metabolizing enzymes [catechol-o-methyltransferase (COMT) and monoamine oxidase A] were found in GC, COMT in GC and thecal cells of large human antral follicles in vivo and in cultured GC. Cellular uptake and metabolism of NE also occurred in cultured GC, events that could be blocked pharmacologically. NE, in the range present in FF, is unlikely to affect GC via activation of typical α- or β-receptors. In line with this assumption, it did not alter phosphorylation of MAPK. However, NE robustly induced the generation of reactive oxygen species (ROS). This action occurred even when receptors were blocked but was prevented by blockers of NE transporter, COMT, and monoamine oxidase A. Thus, NE contributes to the microenvironment of preovulatory human follicles and is lower in PCOS. By inducing the production of ROS in GC, NE is linked to ROS-regulated events, which are emerging as crucial factors in ovarian physiology, including ovulation.

scholarly journals Photoluminescent Gold Nanoclusters in Cancer Cells: Cellular Uptake, Toxicity, and Generation of Reactive Oxygen Species

2017 ◽  
Vol 18 (2) ◽  
pp. 378 ◽  
Author(s):  
Marija Matulionyte ◽  
Dominyka Dapkute ◽  
Laima Budenaite ◽  
Greta Jarockyte ◽  
Ricardas Rotomskis
Keyword(s):  
Reactive Oxygen Species ◽  
Cancer Cells ◽  
Cellular Uptake ◽  
Gold Nanoclusters ◽  
Reactive Oxygen ◽  
Oxygen Species

scholarly journals Non-esterified Fatty Acid-Induced Reactive Oxygen Species Mediated Granulosa Cells Apoptosis Is Regulated by Nrf2/p53 Signaling Pathway

Antioxidants ◽  
2020 ◽  
Vol 9 (6) ◽  
pp. 523
Author(s):  
Yiru Wang ◽  
Chengmin Li ◽  
Julang Li ◽  
Genlin Wang ◽  
Lian Li
Keyword(s):  
Reactive Oxygen Species ◽  
Fatty Acid ◽  
Granulosa Cells ◽  
Ovarian Function ◽  
Reactive Oxygen ◽  
Follicular Development ◽  
Oxygen Species ◽  
Related Protein ◽  
Induced Apoptosis

Negative energy balance (NEB) during the perinatal period can affect dairy cow follicular development and reduce the fecundity. Non-esterified fatty acid (NEFA) concentration is elevated during NEB, and is known to be toxic for multiple cell types. In the ovary, NEB increased NEFA, and may influences follicular growth and development. However, the effect and mechanism of NEFA on granulosa cells (GCs) in vitro remains unknown. In this study, we found that NEFA dose-dependently induced apoptosis in primary cultured granulosa cells. Mechanistically, our data showed that NEFA significantly increased reactive oxygen species (ROS) levels, resulting in the activation of endoplasmic reticulum stress (ERS) and eventually cell apoptosis in GCs. Moreover, NEFA also increased the phosphorylation levels of ERK1/2 and p38MAPK pathways, upregulated the expression of p53 and potentially promoted its translocation to the nuclear, thus transcriptionally activated Bax, a downstream gene of this pathway. NEFA also promoted nuclear factor E2 (Nrf2) expression and its level in the nuclear. To elucidate the mechanism of NEFA action, N-acetyl-l-cysteine (NAC), a ROS scavenger was used to verify the role of ROS in NEFA induced apoptosis of GCs. NAC pretreatment reversed the NEFA-induced ERS-related protein and apoptosis-related protein levels. Meanwhile, NAC pretreatment also blocked the phosphorylation of ERK1/2 and p38 induced by NEFA, and the nucleation of Nrf2 and p53, suggesting that ROS plays a crucial role in regulating the NEFA-induced apoptosis of GCs. Together, these findings provide an improved understanding of the mechanisms underlying GCs apoptosis, which could potentially be useful for improving ovarian function.

Assessment of the predictive value of follicular fluid cytokines and reactive oxygen species in IVF cycles

2002 ◽  
Vol 78 ◽  
pp. S5-S6 ◽  
Author(s):  
Mohamed A Bedaiwy ◽  
Kurt Miller ◽  
Jeffery M Goldberg ◽  
David R Nelson ◽  
Ashok Agarwal ◽  
...  
Keyword(s):  
Reactive Oxygen Species ◽  
Follicular Fluid ◽  
Predictive Value ◽  
Reactive Oxygen ◽  
Oxygen Species
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