scholarly journals Can pubertal boys with Klinefelter syndrome benefit from spermatogonial stem cell banking?

2011 ◽  
Vol 27 (2) ◽  
pp. 323-330 ◽  
Author(s):  
D. Van Saen ◽  
I. Gies ◽  
J. De Schepper ◽  
H. Tournaye ◽  
E. Goossens
Keyword(s):  
Stem Cell ◽  
Klinefelter Syndrome ◽  
Spermatogonial Stem Cell ◽  
Cell Banking ◽  
Pubertal Boys ◽  
Stem Cell Banking

scholarly journals Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation into Azoospermic Torsion Mouse Model

2021 ◽  
Author(s):  
mehrnoush malekzadeh ◽  
Nasrin Takzaree ◽  
heidar toolee ◽  
shokoofe kazemzadeh ◽  
Nasrin khanmohammadi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mouse Model ◽  
Germ Cells ◽  
Positive Influence ◽  
Spermatogonial Stem Cell ◽  
Freezing Medium ◽  
Cell Banking ◽  
Stem Cell Banking ◽  
Cryopreservation Protocol

Abstract Preserving the spermatogonial stem cells (SSCs) in long periods of time during the treatment of male infertility using stem cell banking systems and transplantation is an important issue. Therefore, this study was conducted to develop an optimal cryopreservation protocol for SSCs using 10 mM pentoxifylline (PTX) as an antioxidant in basal freezing medium. Testicular torsion - a mouse model for long-term infertility- was used to transplant fresh SSCs (n = 6), fresh SSCs treated with PTX (n = 6), cryopreserved SSCs with basal freezing medium (n = 6) and cryopreserved SSCs treated with PTX (n = 6). Eight weeks after germ cell transplantation, samples were assessed for proliferation, through evaluation of Ddx4 and Id4 markers, and differentiation via evaluation of C-Kit and Sycp3, Tnp1, Tnp2, and Prm1 markers. According to morphological and flow cytometry results, SSCs are able to form colonies and express Gfra1, ID4, α6‐integrin and β1‐integrin markers. We found positive influence from PTX on proliferative and differentiative markers in SSCs transplanted to azoospermic mice. In the recipient testis, donor SSCs formed spermatogenic colonies and sperm. Respecting these data, adding pentoxifylline is a practical way to precisely cryopreserve germ cells enriched for SSCs in cryopreservation, and this procedure could become an efficient method to restore fertility in a clinical setup. However, more studies are needed to ensure its safety in the long term.

scholarly journals Cryoprotective Effect of Pentoxifylline on Spermatogonial Stem Cell During Transplantation Into Azoospermic Torsion Mouse Model

2021 ◽  
Author(s):  
Mehrnoush Malekzadeh ◽  
Nasrin Takzaree ◽  
Heidar Toolee ◽  
Shokoofeh Kazemzadeh ◽  
Nasrin Khanmohammadi ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mouse Model ◽  
Germ Cells ◽  
Positive Influence ◽  
Spermatogonial Stem Cell ◽  
Freezing Medium ◽  
Cell Banking ◽  
Stem Cell Banking ◽  
Cryopreservation Protocol

Abstract BackgroundPreserving the spermatogonial stem cells (SSCs) in long periods of time during the treatment of male infertility using stem cell banking systems and transplantation is an important issue. Therefore, this study was conducted to develop an optimal cryopreservation protocol for SSCs using 10 mM pentoxifylline (PTX) as an antioxidant in basal freezing medium.MethodsTesticular torsion - a mouse model for long-term infertility- was used to transplant fresh SSCs (n=6), fresh SSCs treated with PTX (n=6), cryopreserved SSCs with basal freezing medium (n=6) and cryopreserved SSCs treated with PTX (n=6). Eight weeks after germ cell transplantation, samples were assessed for proliferation, through evaluation of Ddx4 and Id4 markers, and differentiation via evaluation of C‐Kit and Sycp3, Tnp1, Tnp2, and Prm1 markers.ResultsAccording to morphological and flow cytometry results, SSCs are able to form colonies and express Gfra1, ID4, α6‐integrin and β1‐integrin markers. We found positive influence from PTX on proliferative and differentiative markers in SSCs transplanted to azoospermic mice. In the recipient testis, donor SSCs formed spermatogenic colonies and sperm. ConclusionRespecting these data, adding pentoxifylline is a practical way to precisely cryopreserve germ cells enriched for SSCs in cryopreservation, and this procedure could become an efficient method to restore fertility in a clinical setup. However, more studies are needed to ensure its safety in the long term.

Dental stem cell banking: Techniques and Protocols

2021 ◽  
Author(s):  
Sanaz Khaseb ◽  
Mahdi Orooji ◽  
Majid Ghasemian Pour ◽  
Seyed Mohammadreza Safavi ◽  
Mohammad Jafar Eghbal ◽  
...  
Keyword(s):  
Stem Cell ◽  
Cell Banking ◽  
Stem Cell Banking ◽  
Dental Stem Cell

Perinatal Stem Cells Isolated From Complete Umbilical Cord Tissue for Family Stem Cell Banking and Potential Therapeutic Use

2018 ◽  
pp. 257-269
Author(s):  
Robert Briddell ◽  
Frank Litkenhaus ◽  
James E. Carroll ◽  
Mohammed Ali ◽  
Kate F. Girard ◽  
...  
Keyword(s):  
Stem Cells ◽  
Stem Cell ◽  
Umbilical Cord ◽  
Therapeutic Use ◽  
Cell Banking ◽  
Umbilical Cord Tissue ◽  
Stem Cell Banking
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