scholarly journals Tubal factor infertility is associated with antibodies against Chlamydia trachomatis heat shock protein 60 (HSP60) but not human HSP60

2011 ◽  
Vol 26 (8) ◽  
pp. 2069-2076 ◽  
Author(s):  
A. Hjelholt ◽  
G. Christiansen ◽  
T. G. Johannesson ◽  
H. J. Ingerslev ◽  
S. Birkelund
Keyword(s):  
Heat Shock ◽  
Chlamydia Trachomatis ◽  
Heat Shock Protein ◽  
Heat Shock Protein 60 ◽  
Tubal Factor Infertility ◽  
Tubal Factor ◽  
Human Hsp60

The presence of serum antibody to the chlamydial heat shock protein (CHSP60) as a diagnostic test for tubal factor infertility

1997 ◽  
Vol 67 (3) ◽  
pp. 501-504 ◽  
Author(s):  
Paul Claman ◽  
Lisa Honey ◽  
Rosanna W. Peeling ◽  
Peter Jessamine ◽  
Baldwin Toye
Keyword(s):  
Heat Shock ◽  
Heat Shock Protein ◽  
Diagnostic Test ◽  
Serum Antibody ◽  
Tubal Factor Infertility ◽  
Tubal Factor

scholarly journals Association of Antibodies to Chlamydia trachomatis Heat-Shock Protein 60 kD with Chronic Nongonococcal Urethritis

1997 ◽  
Vol 24 (4) ◽  
pp. 653-660 ◽  
Author(s):  
P. J. Horner ◽  
D. Cain ◽  
M. McClure ◽  
B. J. Thomas ◽  
C. Gilroy ◽  
...  
Keyword(s):  
Heat Shock ◽  
Chlamydia Trachomatis ◽  
Heat Shock Protein ◽  
Heat Shock Protein 60 ◽  
Nongonococcal Urethritis

scholarly journals Immunization with a Peptide Corresponding to Chlamydial Heat Shock Protein 60 Increases the Humoral Immune Response in C3H Mice to a Peptide Representing Variable Domain 4 of the Major Outer Membrane Protein of Chlamydia trachomatis

1999 ◽  
Vol 6 (3) ◽  
pp. 356-363 ◽  
Author(s):  
Vladimir L. Motin ◽  
Luis M. de la Maza ◽  
Ellena M. Peterson
Keyword(s):  
Immune Response ◽  
Cell Proliferation ◽  
T Cell ◽  
Heat Shock ◽  
Chlamydia Trachomatis ◽  
Membrane Protein ◽  
Heat Shock Protein ◽  
T Cell Proliferation ◽  
Heat Shock Protein 60 ◽  
C3h Mice

ABSTRACT C3H (H-2k ) mice are susceptible to a vaginal challenge with human strains of Chlamydia trachomatis and thus are a useful strain for testing potentialChlamydia vaccine candidates. However, C3H mice are fairly poor responders in terms of the level of antibody resulting from immunization with potential protective peptides representing variable domains (VDs) of the major outer membrane protein (MOMP). C57BL/6 (H-2b ) mice, on the other hand, are moderately resistant to a vaginal challenge but are good responders to the chlamydial MOMP VDs. Peptides representing universal T-cell helper epitopes were employed to determine whether the antibody response to a peptide representing VD4 of the MOMP, which has been shown to contain neutralizing epitopes, could be enhanced in C3H and C57 mice. Universal T-cell helper peptides from tetanus toxin, the pre-S2 region of hepatitis B virus, and the mouse heat shock protein 60, as well as the corresponding segment of the Chlamydia heat shock protein 60 (hspct), were coadministered with the VD4 peptide. Peptides were coencapsulated in liposomes containing the adjuvant monophosphoryl lipid A and administered by using a combination of mucosal and intramuscular injection. The only T-cell helper peptide that improved the immune response as judged by antibody level, in vitro neutralization assays, and T-cell proliferation was hspct. The response in the C57BL/6 strain was not significantly enhanced with hspct over levels achieved with VD4 alone; however, in C3H mice the levels of serum antibody to C. trachomatisincreased to that seen in C57 mice. However, the molecular specificity and immunoglobulin subclass distribution differed from those of the C57 response, and the neutralizing titers and T-cell proliferation responses were lower. In both strains of mice, titers of vaginal antibody to C. trachomatis were low. In summary, of the T-helper peptides used, only hspct significantly enhanced the immune response of C3H mice to the VD4 peptide, but it had only a modest effect on the immune response of C57 mice.

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