scholarly journals Ephrin A1 induces intercellular dissociation in Ishikawa cells: possible implication of the Eph-ephrin A system in human embryo implantation

2010 ◽  
Vol 26 (2) ◽  
pp. 299-306 ◽  
Author(s):  
H. Fujii ◽  
H. Fujiwara ◽  
A. Horie ◽  
Y. Sato ◽  
I. Konishi
Keyword(s):  
Human Embryo ◽  
Embryo Implantation ◽  
Ishikawa Cells

scholarly journals Versican V1 in human endometrial epithelial cells promotes BeWo spheroid adhesion in vitro

Reproduction ◽  
2019 ◽  
pp. 53-64 ◽  
Author(s):  
Yumiko Miyazaki ◽  
Akihito Horie ◽  
Hirohiko Tani ◽  
Masashi Ueda ◽  
Asuka Okunomiya ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Chondroitin Sulfate ◽  
Cell Line ◽  
Human Embryo ◽  
Embryo Implantation ◽  
Cancer Cell Line ◽  
Promoting Effect ◽  
Ishikawa Cells ◽  
Endometrial Epithelial Cells

The endometrium extracellular matrix (ECM) is essential for embryo implantation. Versican, a large chondroitin sulfate proteoglycan that binds hyaluronan and forms large ECM aggregates, can influence fundamental physiological phenomena, such as cell proliferation, adhesion and migration. The present study investigated the possible role of versican in human embryo implantation. Versican V1 expression and secretion in human endometrial epithelial cells (EECs) was most prominent in the mid-secretory phase. Versican expression in EECs significantly increased after treatment with estrogen and progesterone, but not by estrogen alone. We also established versican V1-overexpressing Ishikawa (endometrial cancer cell line) cells (ISKW-V1), versican V3-overexpressing (ISKW-V3) and control GFP-overexpressing (ISKW-GFP) Ishikawa cells. By the in vitro implantation model, the attachment ratio of BeWo (choriocarcinoma cell line) spheroids to the monolayer of ISKW-V1, but not of ISKW-V3, was found significantly enhanced compared with attachment to the ISKW-GFP monolayer. The conditioned medium derived from ISKW-V1 (V1-CM) also promoted the attachment of BeWo spheroids to the ISKW monolayer. However, this attachment-promoting effect was abolished when V1-CM was pretreated with chondroitinase ABC, which degrades chondroitin sulfate. Therefore, out of the ECM components, versican V1 may facilitate human embryo implantation.

Endometrial Receptivity and Human Embryo Implantation

2011 ◽  
Vol 66 ◽  
pp. 23-30 ◽  
Author(s):  
Najwa A. Rashid ◽  
Sujata Lalitkumar ◽  
Parameswaran G. Lalitkumar ◽  
Kristina Gemzell-Danielsson
Keyword(s):  
Human Embryo ◽  
Embryo Implantation ◽  
Endometrial Receptivity

scholarly journals Characterisation of Osteopontin in an In Vitro Model of Embryo Implantation

Cells ◽  
2019 ◽  
Vol 8 (5) ◽  
pp. 432 ◽  
Author(s):  
Stéphane C Berneau ◽  
Peter T Ruane ◽  
Daniel R Brison ◽  
Susan J Kimber ◽  
Melissa Westwood ◽  
...  
Keyword(s):  
Secretory Pathway ◽  
Embryo Implantation ◽  
Giant Cells ◽  
Ishikawa Cell ◽  
Altered Expression ◽  
Ishikawa Cells ◽  
Cell Layers ◽  
Endometrial Epithelium ◽  
Vitro Model

At the onset of pregnancy, embryo implantation is initiated by interactions between the endometrial epithelium and the outer trophectoderm cells of the blastocyst. Osteopontin (OPN) is expressed in the endometrium and is implicated in attachment and signalling roles at the embryo–epithelium interface. We have characterised OPN in the human endometrial epithelial Ishikawa cell line using three different monoclonal antibodies, revealing at least nine distinct molecular weight forms and a novel secretory pathway localisation in the apical domain induced by cell organisation into a confluent epithelial layer. Mouse blastocysts co-cultured with Ishikawa cell layers served to model embryo apposition, attachment and initial invasion at implantation. Exogenous OPN attenuated initial, weak embryo attachment to Ishikawa cells but did not affect the attainment of stable attachment. Notably, exogenous OPN inhibited embryonic invasion of the underlying cell layer, and this corresponded with altered expression of transcription factors associated with differentiation from trophectoderm (Gata2) to invasive trophoblast giant cells (Hand1). These data demonstrate the complexity of endometrial OPN forms and suggest that OPN regulates embryonic invasion at implantation by signalling to the trophectoderm.

A Model for Human Embryo Implantation Using Trophoblast Generated from Embryonic Stem Cells.

2008 ◽  
Vol 78 (Suppl_1) ◽  
pp. 57-57
Author(s):  
Ramya Udayashankar ◽  
Claire Kershaw-Young ◽  
Elizabeth Tuckerman ◽  
Duncan Baker ◽  
Susan Laird ◽  
...  
Keyword(s):  
Stem Cells ◽  
Embryonic Stem Cells ◽  
Human Embryo ◽  
Embryo Implantation ◽  
Embryonic Stem

scholarly journals A Novel Concept of Fundus-Ovary-Salpinx-Para-Aorta Implantation Promoting Unit during Human Embryo Implantation

10.5772/60634 ◽  
2015 ◽  
Author(s):  
Hiroshi Fujiwara ◽  
Yoshihiko Araki ◽  
Shigeru Saito ◽  
Kazuhiko Imakawa ◽  
Satoru Kyo ◽  
...  
Keyword(s):  
Human Embryo ◽  
Embryo Implantation ◽  
Novel Concept
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