scholarly journals Effect of the method of conception and embryo transfer procedure on mid-gestation placenta and fetal development in an IVF mouse model

2010 ◽  
Vol 25 (8) ◽  
pp. 2039-2046 ◽  
Author(s):  
L. Delle Piane ◽  
W. Lin ◽  
X. Liu ◽  
A. Donjacour ◽  
P. Minasi ◽  
...  
Keyword(s):  
Mouse Model ◽  
Embryo Transfer ◽  
Fetal Development

265. Gonadotrophic hormones affect the uterus, implantation and fetal development in mice

2005 ◽  
Vol 17 (9) ◽  
pp. 108
Author(s):  
R. L. Kelley ◽  
K. L. Kind ◽  
M. Lane ◽  
R. L. Robker ◽  
J. G. Thompson ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Pregnancy Outcomes ◽  
Fetal Development ◽  
Reproductive Tract ◽  
Homeobox Gene ◽  
Chorionic Gonadotrophin ◽  
Leukaemia Inhibitory Factor ◽  
Mrna Levels ◽  
Protein Distribution ◽  
Plasma Progesterone

Although gonadotrophin stimulation using equine chorionic gonadotrophin (eCG) adversely influences pregnancy and fetal development, the effects of stimulation using recombinant human follicle stimulating hormone (rhFSH) are largely unknown. Evidence from human studies indicates that rhFSH may also be detrimental to the endometrium and implantation. We investigated the effect of gonadotrophin stimulation on ovarian hormones and uterine characteristics in the peri-implantation period, and pregnancy outcomes in mice. Adult female mice were stimulated with 2.5 IU or 10 IU rhFSH or 5 IU eCG, followed by 5 IU human chorionic gonadotrophin (hCG). Control mice received saline injections. On day 4 of pseudopregnancy mice either had embryos transferred to the uterus or were sacrificed and blood and uterine samples collected. Plasma progesterone and estradiol concentrations were determined by radioimmunoassay. Uterine mRNA levels of the estrogen and progesterone receptors (ERa and PR), leukaemia inhibitory factor (LIF), homeobox gene Hoxa10 and vascular endothelial growth factor (VEGF) were determined by real-time RT-PCR. Uterine protein distribution of PR was determined by immunohistochemistry. Embryo transfer recipients were sacrificed on day 15 to assess pregnancy outcomes. Gonadotrophin stimulation increased plasma progesterone concentration compared to controls, while estradiol concentrations were not affected. Stimulation also reduced total LIF mRNA and altered the spatial distribution of PR protein in the uterus on day 4. Embryo transfer recipients administered eCG or 10IU rhFSH had lower pregnancy rates compared to controls (11, 35 and 75% respectively) and fetuses from the rhFSH group had reduced mean weight (94 ± 6 v. 176 ± 8 mg), length (11 ± 0.2 v. 12 ± 0.1 mm) and maturity (14.1 ± 0.09 v. 14.6 ± 0.05 days) compared to controls. These results demonstrate that gonadotrophin stimulation induces changes to the maternal reproductive tract prior to implantation that have consequences for the establishment of pregnancy and fetal development in the mouse.

scholarly journals Utero-tubal Embryo Transfer and Vasectomy in the Mouse Model

10.3791/51214 ◽  
2014 ◽  
Author(s):  
Pablo Bermejo-Alvarez ◽  
Ki-Eun Park ◽  
Bhanu P. Telugu
Keyword(s):  
Mouse Model ◽  
Embryo Transfer ◽  
Tubal Embryo Transfer

Severe thrombocytopenia is sufficient for fetal and neonatal intra-cerebral hemorrhage to occur

Blood ◽  
2021 ◽  
Author(s):  
Alison Farley ◽  
Sarah Lloyd ◽  
Merle Dayton ◽  
Christine Biben ◽  
Olivia Stonehouse ◽  
...  
Keyword(s):  
Mouse Model ◽  
Fetal Development ◽  
Cerebral Hemorrhage ◽  
Severe Thrombocytopenia ◽  
Mouse Line ◽  
Cerebral Vasculature ◽  
Neurological Damage ◽  
Neonatal Life ◽  
The Brain ◽  
Neonatal Population

Intra-cerebral hemorrhage (ICH) has a devastating impact on the neonatal population. Whether thrombocytopenia is sufficient to cause ICH in neonates is debated. In this study we have comprehensively investigated the consequences of severe thrombocytopenia on the integrity of the cerebral vasculature using two orthogonal approaches: genetically, by studying embryogenesis in the Nfe2-/- mouse line; and using biologics (anti-GP1Ba antibodies) to induce severe thrombocytopenia at defined times during development. Using a mouse model, we present data herein that not only demonstrate that platelets are required throughout fetal development and into neonatal life to maintain the integrity of the cerebral vasculature to prevent hemorrhage, but also that the location of cerebral hemorrhage is dependent on the timing during development when thrombocytopenia occurs. Importantly, this study demonstrates that thrombocytopenia-associated fetal/neonatal ICH occurs within regions of the brain which, in humans, could lead to neurological damage.

The effects of post‐frozen‐thawed embryo transfer pregnancy on early fetal development

2022 ◽  
Author(s):  
Naoko Suga ◽  
Mari S. Oba ◽  
Iwaho Kikuchi ◽  
Michiya Natori ◽  
Kazuhiro Hisamatsu ◽  
...  
Keyword(s):  
Embryo Transfer ◽  
Fetal Development ◽  
Frozen Thawed Embryo Transfer ◽  
Thawed Embryo Transfer

Mouse embryo co-culture with autologous cumulus cells and fetal development post-embryo transfer

2011 ◽  
Vol 82 (3) ◽  
pp. 420-427 ◽  
Author(s):  
Farha Naomi OMAR FAROUK ◽  
David STOTT ◽  
Marcela VLAD
Keyword(s):  
Embryo Transfer ◽  
Mouse Embryo ◽  
Fetal Development ◽  
Cumulus Cells

Regenerating myofibers in tibialis anterior muscles of young ‘MDX’ mice

1987 ◽  
Vol 45 ◽  
pp. 726-727
Author(s):  
H. D. Geissinge ◽  
L.D. Rhodes
Keyword(s):  
Muscular Dystrophy ◽  
Mouse Model ◽  
Tibialis Anterior ◽  
Physiological Activity ◽  
Mdx Mice ◽  
Mode Of Inheritance

A recently discovered mouse model (‘mdx’) for muscular dystrophy in man may be of considerable interest, since the disease in ‘mdx’ mice is inherited by the same mode of inheritance (X-linked) as the human Duchenne (DMD) muscular dystrophy. Unlike DMD, which results in a situation in which the continual muscle destruction cannot keep up with abortive regenerative attempts of the musculature, and the sufferers of the disease die early, the disease in ‘mdx’ mice appears to be transient, and the mice do not die as a result of it. In fact, it has been reported that the severely damaged Tibialis anterior (TA) muscles of ‘mdx’ mice seem to display exceptionally good regenerative powers at 4-6 weeks, so much so, that these muscles are able to regenerate spontaneously up to their previous levels of physiological activity.

Mouse model of gastric infection with cytotoxin-expressing strain of Helicobacter pylori in studying of pathogenesis of chronic gastric ulcer

1998 ◽  
Vol 13 (11-s4) ◽  
pp. S178-S184 ◽  
Author(s):  
PETER KONTUREK ◽  
TOMASZ BRZOZOWSKI ◽  
STANISLAW KONTUREK ◽  
ELZBIETA KARCZEWSKA ◽  
ROBERT PAJDO ◽  
...  
Keyword(s):  
Helicobacter Pylori ◽  
Gastric Ulcer ◽  
Mouse Model ◽  
Chronic Gastric Ulcer
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