scholarly journals An in vivo culture system for human embryos using an encapsulation technology: a pilot study

2008 ◽  
Vol 24 (4) ◽  
pp. 790-796 ◽  
Author(s):  
C. Blockeel ◽  
P. Mock ◽  
G. Verheyen ◽  
N. Bouche ◽  
Ph. Le Goff ◽  
...  
Keyword(s):  
Pilot Study ◽  
Culture System ◽  
Human Embryos ◽  
In Vivo Culture

Application of an in vivo culture system for rapid demonstration of anti-Leishmania activity of monoclonal antibodies

1987 ◽  
Vol 81 (2) ◽  
pp. 210-211 ◽  
Author(s):  
L. Monjour ◽  
I. Vouldoukis ◽  
M.C. Debons-Guillemin ◽  
C. Alfred ◽  
C. Chopin ◽  
...  
Keyword(s):  
Monoclonal Antibodies ◽  
Culture System ◽  
In Vivo Culture

scholarly journals Effect of plasma from cyclophosphamide-treated mice on CFU-S in an in vivo culture system

Blood ◽  
1980 ◽  
Vol 56 (5) ◽  
pp. 940-942
Author(s):  
W Fried ◽  
J Barone-Varelas ◽  
M Helfgott ◽  
M Berman
Keyword(s):  
Cell Suspension ◽  
Peritoneal Cavity ◽  
Culture System ◽  
Normal Mouse ◽  
Alternative Explanation ◽  
Marrow Cell ◽  
Initial Value ◽  
Dialysis Tubing ◽  
In Vivo Culture

Various studies suggest that humoral substances, capable of stimulating CFU-S proliferation, are released into the plasma in response to depletion of the CFU-S population by cytotoxic substances such as cyclophosphamide. To test this hypothesis, we placed 0.25 ml of a murine marrow cell suspension with an equal volume of plasma from either normal mice or from mice previously injected with 5 mg of cyclophosphamide into cellulose dialysis tubing. These tubes were then incubated in the peritoneal cavity of mice for 1–7 days. The CFU-S content of the tubes was then assayed. The CFu-S content of suspensions in normal mouse plasma declined to one-fourth of the initial value after 7 days, whereas those in plasma from mice that received cyclophosphamide 7 days previously were essentially unchanged in number. These data suggest that 7 days after injection of cyclophosphamide, the plasma contains a factor that either prevents death of CFU-S or stimulates them to proliferate. An alternative explanation is that normal plasma contains an inhibitor of CFU-S growth that is lacking in plasma of cyclophosphamide-treated mice.

(22) An in vivo culture system for human papillomavirus-infected keratinocytes

1989 ◽  
Vol 121 (s34) ◽  
pp. 64-65
Author(s):  
J.C. Sterling ◽  
A.C. Minson ◽  
M.A. Stanley
Keyword(s):  
Human Papillomavirus ◽  
Culture System ◽  
In Vivo Culture

scholarly journals Effect of a congenital defect in hemopoiesis on myeloid growth and the stem cell (CFU) in an in vivo culture system

Blood ◽  
1976 ◽  
Vol 47 (3) ◽  
pp. 413-421 ◽  
Author(s):  
WS Tyler ◽  
F Jr Stohlman ◽  
M Chovaniec ◽  
D Howard
Keyword(s):  
Stem Cell ◽  
Culture System ◽  
Control Level ◽  
Spillover Effect ◽  
Diffusion Chamber ◽  
Defect Diffusion ◽  
In Vivo Culture ◽  
Cell Defect ◽  
Humoral Control

Abstract W/Wv mice with congenitally defective CFU proliferation and their normal, congenic littermates were used as hosts for diffusion chamber (DC) implants. CFU growth in implanted allogenic CF1, or congenic +/+ marrow was significantly greater in W/Wv than in control hosts. When W/Wv mice were “cured” of their hemopoietic defect, CFU proliferation in the DCs decreased, but not to the control level. These observations have provided evidence for humoral control of CFU growth related to a genetic stem cell defect. Diffusion chamber myelopoiesis was also enhanced in W/Wv hosts. In comparison with their congenic controls, W/Wv mice were neutropenic and had decreased numbers of marrow myeloid elements. Thus, a humorally mediated feedback related to a defective myelopoiesis in the hosts might have accounted for increased DC myelopoiesis. However, a “spillover”effect from increased stem cell growth has not been excluded.

scholarly journals Effect of plasma from cyclophosphamide-treated mice on CFU-S in an in vivo culture system

Blood ◽  
1980 ◽  
Vol 56 (5) ◽  
pp. 940-942 ◽  
Author(s):  
W Fried ◽  
J Barone-Varelas ◽  
M Helfgott ◽  
M Berman
Keyword(s):  
Cell Suspension ◽  
Peritoneal Cavity ◽  
Culture System ◽  
Normal Mouse ◽  
Alternative Explanation ◽  
Marrow Cell ◽  
Initial Value ◽  
Dialysis Tubing ◽  
In Vivo Culture

Abstract Various studies suggest that humoral substances, capable of stimulating CFU-S proliferation, are released into the plasma in response to depletion of the CFU-S population by cytotoxic substances such as cyclophosphamide. To test this hypothesis, we placed 0.25 ml of a murine marrow cell suspension with an equal volume of plasma from either normal mice or from mice previously injected with 5 mg of cyclophosphamide into cellulose dialysis tubing. These tubes were then incubated in the peritoneal cavity of mice for 1–7 days. The CFU-S content of the tubes was then assayed. The CFu-S content of suspensions in normal mouse plasma declined to one-fourth of the initial value after 7 days, whereas those in plasma from mice that received cyclophosphamide 7 days previously were essentially unchanged in number. These data suggest that 7 days after injection of cyclophosphamide, the plasma contains a factor that either prevents death of CFU-S or stimulates them to proliferate. An alternative explanation is that normal plasma contains an inhibitor of CFU-S growth that is lacking in plasma of cyclophosphamide-treated mice.

scholarly journals Chick limbs with mouse teeth: An effective in vivo culture system for tooth germ development and analysis

2002 ◽  
Vol 226 (1) ◽  
pp. 149-154 ◽  
Author(s):  
Eiki Koyama ◽  
Changshan Wu ◽  
Tsuyoshi Shimo ◽  
Maurizio Pacifici
Keyword(s):  
Culture System ◽  
Tooth Germ ◽  
Tooth Germ Development ◽  
In Vivo Culture

scholarly journals Effect of a congenital defect in hemopoiesis on myeloid growth and the stem cell (CFU) in an in vivo culture system

Blood ◽  
1976 ◽  
Vol 47 (3) ◽  
pp. 413-421 ◽  
Author(s):  
WS Tyler ◽  
F Jr Stohlman ◽  
M Chovaniec ◽  
D Howard
Keyword(s):  
Stem Cell ◽  
Culture System ◽  
Control Level ◽  
Spillover Effect ◽  
Diffusion Chamber ◽  
Defect Diffusion ◽  
In Vivo Culture ◽  
Cell Defect ◽  
Humoral Control

W/Wv mice with congenitally defective CFU proliferation and their normal, congenic littermates were used as hosts for diffusion chamber (DC) implants. CFU growth in implanted allogenic CF1, or congenic +/+ marrow was significantly greater in W/Wv than in control hosts. When W/Wv mice were “cured” of their hemopoietic defect, CFU proliferation in the DCs decreased, but not to the control level. These observations have provided evidence for humoral control of CFU growth related to a genetic stem cell defect. Diffusion chamber myelopoiesis was also enhanced in W/Wv hosts. In comparison with their congenic controls, W/Wv mice were neutropenic and had decreased numbers of marrow myeloid elements. Thus, a humorally mediated feedback related to a defective myelopoiesis in the hosts might have accounted for increased DC myelopoiesis. However, a “spillover”effect from increased stem cell growth has not been excluded.

scholarly journals In Vivo Culture System Using the INVOcell Device Shows Similar Pregnancy and Implantation Rates to those Obtained from in Vivo Culture System in ICSI Procedures

2015 ◽  
Vol 9 ◽  
pp. CMRH.S25494 ◽  
Author(s):  
Javier García-Ferreyra ◽  
Roly Hilario ◽  
Daniel Luna ◽  
Lucy Villegas ◽  
Rocío Romero ◽  
...  
Keyword(s):  
Clinical Outcomes ◽  
Culture System ◽  
Embryo Quality ◽  
Implantation Rate ◽  
Control Group ◽  
Study Group ◽  
Miscarriage Rate ◽  
In Vivo Culture ◽  
And Control

Capsule Clinical outcomes using INVOcell device with ICSI. Objective Intravaginal culture of oocytes (INVO) procedure is an intravaginal culture system that utilizes the INVOcell device in which the fertilization and embryo culture occur. In this procedure, the vaginal cavity serves as an incubator for oocyte fertilization and early embryonic development. The objective of this study was to evaluate the clinical outcomes of this intravaginal culture system in intracytoplasmic sperm injection (ICSI). METHODS: A total of 24 cycles INVO-ICSI (study group) and 74 cycles of ICSI (control group) were included in the study. The cleaved oocytes at day 3/ total injected oocytes, embryo quality, pregnancy rate (PR), implantation rate (IR), and miscarriage rate (MR) were compared between both groups. Results At day 3, there was no difference in the cleaved oocyte rate (78.7 and 76.1%) and embryo quality (77 and 86.8%) for the study and control groups, respectively. In the study group, more embryos were significantly transferred compared to the control group (2.63 ± 0.58 versus 1.93 ± 0.25; P < 0.05). PRs, IRs, and MRs were similar for the study group compared with the control group (PR: 54.2% versus 58.1%; IR: 31.7% versus 33.6%; MR: 7.7% versus 20.9%). Conclusions Good PR and IR can be obtained using the INVOcell device, and the INVO-ICSI procedure can be considered as an alternative option to infertile patients.

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