scholarly journals Expression of secretory leukocyte protease inhibitor and elafin in human fallopian tube and in an in-vitro model of Chlamydia trachomatis infection

2008 ◽  
Vol 24 (3) ◽  
pp. 679-686 ◽  
Author(s):  
A. E. King ◽  
N. Wheelhouse ◽  
S. Cameron ◽  
S. E. McDonald ◽  
K.-F. Lee ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Protease Inhibitor ◽  
Fallopian Tube ◽  
In Vitro Model ◽  
Secretory Leukocyte Protease Inhibitor ◽  
Chlamydia Trachomatis Infection ◽  
Human Fallopian Tube ◽  
Vitro Model

Chlamydia trachomatis enhances the expression of matrix metalloproteinases in an in vitro model of the human fallopian tube infection

2002 ◽  
Vol 187 (5) ◽  
pp. 1377-1383 ◽  
Author(s):  
Kevin A. Ault ◽  
Kathleen A. Kelly ◽  
Paul E. Ruther ◽  
Angelo A. Izzo ◽  
Linda S. Izzo ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Matrix Metalloproteinases ◽  
Fallopian Tube ◽  
In Vitro Model ◽  
Human Fallopian Tube ◽  
Vitro Model

scholarly journals An in vitro model of azithromycin-induced persistent Chlamydia trachomatis infection

2017 ◽  
Vol 364 (14) ◽  
Author(s):  
Yaohua Xue ◽  
Heping Zheng ◽  
Zhida Mai ◽  
Xiaolin Qin ◽  
Wentao Chen ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
In Vitro Model ◽  
Chlamydia Trachomatis Infection ◽  
Vitro Model

scholarly journals An in vitro Model of Chlamydia trachomatis Infection in the Regenerative Phase of the Human Endometrial Cycle

Microbiology ◽  
1988 ◽  
Vol 134 (7) ◽  
pp. 2077-2087 ◽  
Author(s):  
S. Campbell ◽  
S. J. Richmond ◽  
P. Haynes ◽  
D. Gump ◽  
P. Yates ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
In Vitro Model ◽  
Chlamydia Trachomatis Infection ◽  
Vitro Model ◽  
Regenerative Phase

scholarly journals Chlamydia trachomatis infection of human fallopian tube organ cultures

1990 ◽  
Vol 136 (6) ◽  
pp. 1109-1115 ◽  
Author(s):  
M. D. Cooper ◽  
J. Rapp ◽  
C. Jeffery-Wiseman ◽  
R. C. Barnes ◽  
D. S. Stephens
Keyword(s):  
Chlamydia Trachomatis ◽  
Fallopian Tube ◽  
Organ Cultures ◽  
Chlamydia Trachomatis Infection ◽  
Human Fallopian Tube

scholarly journals Potential IFNγ Modulation of Inflammasome Pathway in Chlamydia trachomatis Infected Synovial Cells

Life ◽  
2021 ◽  
Vol 11 (12) ◽  
pp. 1359
Author(s):  
Simone Filardo ◽  
Marisa Di Pietro ◽  
Federica Frasca ◽  
Fabiana Diaco ◽  
Mirko Scordio ◽  
...  
Keyword(s):  
Chlamydia Trachomatis ◽  
Synovial Cells ◽  
Chlamydia Trachomatis Infection ◽  
Host Cell Response ◽  
Caspase 1 ◽  
Ifn Γ ◽  
Subsequent Activation ◽  
Vitro Model ◽  
First Time

Following a Chlamydia trachomatis infection, the host immune response is characterized by its recognition via Toll-like and Nod-like Receptors, and the subsequent activation of interferon (IFN)-γ-mediated signaling pathways. Recently, the inflammasome-mediated host cell response has emerged to play a role in the physiopathology of C. trachomatis infection. Here we investigated, for the first time, the interaction of IFN-γ and inflammasome in an in vitro model of C. trachomatis-infected primary human synovial cells. Chlamydial replication as well as the expression of caspase-1, IL-1β, as well as IL-18 and IL-6, were assayed. Our results demonstrated the inhibitory activity of IFN-γ by interfering with the inflammasome network through the downregulation of caspase-1 mRNA expression. In addition, the ability of C. trachomatis to hinder the inflammasome pathway favoring its intracellular survival within synovial cells, was observed. Overall, our data suggest a potential mechanism of immune evasion by C. trachomatis in synovial cells, that may be contested by IFN-γ.

An in vitro model for investigation of vitamin A effects on wound healing

2021 ◽  
pp. 1-6
Author(s):  
Hoda Keshmiri Neghab ◽  
Mohammad Hasan Soheilifar ◽  
Gholamreza Esmaeeli Djavid
Keyword(s):  
Wound Healing ◽  
Endothelial Cell ◽  
Vitamin A ◽  
In Vitro Model ◽  
Cellular Proliferation ◽  
Endothelial Cell Migration ◽  
Normal Fibroblast ◽  
Anti Inflammatory ◽  
Vitro Model

Abstract. Wound healing consists of a series of highly orderly overlapping processes characterized by hemostasis, inflammation, proliferation, and remodeling. Prolongation or interruption in each phase can lead to delayed wound healing or a non-healing chronic wound. Vitamin A is a crucial nutrient that is most beneficial for the health of the skin. The present study was undertaken to determine the effect of vitamin A on regeneration, angiogenesis, and inflammation characteristics in an in vitro model system during wound healing. For this purpose, mouse skin normal fibroblast (L929), human umbilical vein endothelial cell (HUVEC), and monocyte/macrophage-like cell line (RAW 264.7) were considered to evaluate proliferation, angiogenesis, and anti-inflammatory responses, respectively. Vitamin A (0.1–5 μM) increased cellular proliferation of L929 and HUVEC (p < 0.05). Similarly, it stimulated angiogenesis by promoting endothelial cell migration up to approximately 4 fold and interestingly tube formation up to 8.5 fold (p < 0.01). Furthermore, vitamin A treatment was shown to decrease the level of nitric oxide production in a dose-dependent effect (p < 0.05), exhibiting the anti-inflammatory property of vitamin A in accelerating wound healing. These results may reveal the therapeutic potential of vitamin A in diabetic wound healing by stimulating regeneration, angiogenesis, and anti-inflammation responses.

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