scholarly journals Evaluation of Ki-67 antigen expression in the zona reticularis of the adrenal cortex of female rats in persistent estrus

2008 ◽  
Vol 24 (3) ◽  
pp. 705-709 ◽  
Author(s):  
B. B. da Silva ◽  
P. V. Lopes-Costa ◽  
A. R. dos Santos ◽  
C. G. Pires ◽  
C. S. Borges ◽  
...  
Keyword(s):  
Adrenal Cortex ◽  
Antigen Expression ◽  
Female Rats ◽  
Ki 67 ◽  
Zona Reticularis ◽  
Persistent Estrus

Ki-67 antigen expression in the mammary epithelium of female rats in persistent estrus treated with anastrozole

2017 ◽  
Vol 33 (5) ◽  
pp. 359-362 ◽  
Author(s):  
Maria da Conceição Barros-Oliveira ◽  
Danylo Rafhael Costa-Silva ◽  
Danielle Benigno de Andrade ◽  
Umbelina Soares Borges ◽  
Vladimir Costa Silva ◽  
...  
Keyword(s):  
Mammary Epithelium ◽  
Antigen Expression ◽  
Female Rats ◽  
Ki 67 ◽  
Persistent Estrus

Comparative Study of Tamoxifen and Raloxifene on Endometrial Cell Proliferation of Female Rats in Persistent Estrus

2012 ◽  
Vol 22 (1) ◽  
pp. 30-34 ◽  
Author(s):  
Dorival Mendes Rodrigues Junior ◽  
Alesse Ribeiro dos Santos ◽  
Pedro Vitor Lopes Costa ◽  
Benedito Borges da Silva
Keyword(s):  
Protein Expression ◽  
Immunohistochemical Analysis ◽  
Antigen Expression ◽  
Female Rats ◽  
Ki 67 ◽  
Subcutaneous Dose ◽  
Persistent Estrus ◽  
Group 3 ◽  
Group 2 ◽  
Endometrial Epithelium

ObjectiveThe objective of the study was to compare the effect of tamoxifen and raloxifene on the endometrium of female rats in persistent estrus, by Ki-67 protein expression.MethodsThe study comprised 60 Wistar-Hannover female rats in persistent estrus, induced by a single subcutaneous dose of 1.25 mg of testosterone propionate on the second day of age. At 90 days of life, the animals were randomly divided into 3 groups of 20 animals each. Group 1 (control), received only placebo; group 2, the animals were treated with tamoxifen, 250 μg/d; and group 3, the rats were treated with 750 μg/d of raloxifene by gavage during 30 days. Then, the animals were killed, and the endometrium was removed for immunohistochemical analysis of Ki-67 antigen expression. Statistical analysis was performed by β regression model (P < 0.05).ResultsMean percentages of Ki-67 protein expression in the endometrium of rats in persistent estrus were 43.21% ± 3.39%, 7.36% ± 0.95%, and 7.20% ± 0.76% in groups 1, 2 and 3, respectively (P < 0.001). There was no statistical difference between groups 2 and 3 (P = 0.7159).ConclusionsThe present results indicate that, at the doses and during the time of treatment used, both tamoxifen and raloxifene induce atrophy in a similar way of endometrial epithelium of rats in persistent estrus.

scholarly journals Effects of anastrozole on Ki-67 antigen expression in the vaginal epithelium of female rats in persistent estrus

Clinics ◽  
2020 ◽  
Vol 75 ◽  
Author(s):  
Diego Cipriano Chagas ◽  
Maria da Conceição Barros-Oliveira ◽  
Pedro Vitor Lopes-Costa ◽  
Renato de Oliveira Pereira ◽  
Mariella de Almeida Melo ◽  
...  
Keyword(s):  
Antigen Expression ◽  
Vaginal Epithelium ◽  
Female Rats ◽  
Ki 67 ◽  
Persistent Estrus

Evaluation of Ki-67 antigen expression in the vaginal epithelium of castrated female rats treated with raloxifene and tamoxifen: a comparative study

2016 ◽  
Vol 32 (6) ◽  
pp. 453-456
Author(s):  
Adinaide C. de Moura-Leal ◽  
João Macedo de Sousa ◽  
Heyder de Moura-Leal ◽  
Gabriela V. de Sousa ◽  
Airlane P. Alencar ◽  
...  
Keyword(s):  
Comparative Study ◽  
Antigen Expression ◽  
Vaginal Epithelium ◽  
Female Rats ◽  
Ki 67

THE PITUITARY AND ADRENAL RESPONSES TO ADMINISTRATION OF OESTRIOL IN GONADECTOMIZED RATS

1961 ◽  
Vol 38 (1) ◽  
pp. 50-58 ◽  
Author(s):  
N. E. Borglin ◽  
L. Bjersing
Keyword(s):  
Pituitary Gland ◽  
Adrenal Cortex ◽  
Clinical Experience ◽  
Uterine Cervix ◽  
Morphological Changes ◽  
Physiological Significance ◽  
Female Rats ◽  
Experimental Conditions ◽  
Male And Female ◽  
Male And Female Rats

ABSTRACT Oestriol (oestra-1,3,5(10)-triene-3,16α,17β-triol) is a weakly oestrogenic substance which, however, in contrast to what was formerly believed, is of physiological significance. Its effect is localized largely to the uterine cervix and vagina. Clinical experience argues both for and against an effect on the pituitary gland. This investigation is concerned with the morphological changes in the pituitary gland and adrenal cortex of gonadectomized male and female rats after the injection of oestriol. It was found that oestriol has the same type of action on these glands as other oestrogens, but under the experimental conditions used, this effect proved much weaker than that produced by oestradiol (oestra-1,3,5(10)-triene-3,17β-diol).

PROPERTIES OF RAT ADRENAL ZONA RETICULARIS CELLS: PRODUCTION AND STIMULATION OF CERTAIN STEROIDS

1979 ◽  
Vol 83 (3) ◽  
pp. 435-447 ◽  
Author(s):  
J. B. G. BELL ◽  
R. P. GOULD ◽  
P. J. HYATT ◽  
J. F. TAIT ◽  
S. A. S. TAIT
Keyword(s):  
Adrenal Cortex ◽  
Significant Role ◽  
Zona Fasciculata ◽  
Cell Type ◽  
Acth Stimulation ◽  
Specific Stimulus ◽  
Zona Reticularis ◽  
Cell Pool ◽  
Stimulation Of

The outputs of corticosterone, deoxycorticosterone and androstenedione from dispersed, purified rat adrenal zona reticularis and zona fasciculata cells have been measured by radioimmunoassay. Preferential production of deoxycorticosterone by zona reticularis cells was demonstrated by their higher basal deoxycorticosterone: corticosterone ratio when compared with zona fasciculata cells. Adrenocorticotrophin (ACTH) stimulated corticosterone output by all cell pools prepared by unit gravity (1 g) sedimentation, zona fasciculata cells being stimulated 130-fold compared with 20-fold for the zona reticularis cells in relation to their basal corticosterone output. In every cell pool, ACTH stimulated the output of corticosterone more than it stimulated the output of deoxycorticosterone. In parallel cell preparations, it was shown that ACTH increased the conversion of tracer amounts of radioactive deoxycorticosterone to corticosterone and decreased the conversion of radioactive corticosterone to 11-dehydrocorticosterone. Adrenocorticotrophin did not increase the conversion of radioactive deoxycorticosterone to total 11-oxygenated steroids (corticosterone+ 11-dehydrocorticosterone). It is unlikely therefore that ACTH stimulates 11 β-hydroxylation. Data indicate that the ratio of deoxycorticosterone to total 11-oxygenated steroids (corticosterone +11-dehydrocorticosterone) is characteristic for each cell type, and that this ratio will be relatively independent of ACTH stimulation or the amount of pregnenolone substrate available. Basal androstenedione outputs were similar for both types of cell, and ACTH stimulation was very small, being slightly greater for zona fasciculata than for zona reticularis cells. The contribution of the zona reticularis cells to the basal output of any steroid by the cells of the inner two zones of the adrenal cortex of the rat was relatively small (20% for deoxycorticosterone and 10% for corticosterone) and was even less after stimulation by ACTH. Unless a specific stimulus can be found, therefore, a significant role for the zona reticularis cannot yet be established.

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