Behavioral response of human spermatozoa to a concentration jump of chemoattractants or intracellular cyclic nucleotides

Anna Gakamsky; Leah Armon; Michael Eisenbach

doi:10.1093/humrep/den409

Behavioral response of human spermatozoa to a concentration jump of chemoattractants or intracellular cyclic nucleotides

Human Reproduction ◽

10.1093/humrep/den409 ◽

2009 ◽

Vol 24 (5) ◽

pp. 1152-1163 ◽

Cited By ~ 28

Author(s):

Anna Gakamsky ◽

Leah Armon ◽

Michael Eisenbach

Keyword(s):

Cyclic Nucleotides ◽

Behavioral Response ◽

Human Spermatozoa ◽

Concentration Jump

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DNA fragmentation of human spermatozoa: Simple assessment of single‐ and double‐strand DNA breaks and their respective dynamic behavioral response

Andrology ◽

10.1111/andr.12819 ◽

2020 ◽

Vol 8 (5) ◽

pp. 1287-1303

Author(s):

Ana Tímermans ◽

Rosana Vázquez ◽

Fátima Otero ◽

Jaime Gosálvez ◽

Stephen Johnston ◽

...

Keyword(s):

Dna Fragmentation ◽

Behavioral Response ◽

Human Spermatozoa ◽

Dna Breaks ◽

Double Strand Dna Breaks ◽

Double Strand Dna

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The Fine Structure of Glycocalyx in Human Spermatozoa. A High Resolution Cytochemical Study

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100073647 ◽

1973 ◽

Vol 31 ◽

pp. 640-641

Author(s):

P. Hernández-Jáuregui ◽

A. Sosa ◽

A. González Angulo

Keyword(s):

Negative Charge ◽

Iron Hydroxide ◽

Human Spermatozoa ◽

Surface Coat ◽

Cell Surfaces ◽

Colloidal Iron ◽

Cytochemical Study ◽

Specific Permeability ◽

Extracellular Glycoprotein ◽

Mammalian Spermatozoa

Glycocalyx is the name given by Bennett to the extracellular glycoprotein coat present in some cell surfaces. It appears to play an important role in cell properties such as antigenicity, cell adhesivity, specific permeability, and ATP ase activity. In the sperm this coat can be directly related to such important phenomena as capacitation and fertilization. The presence of glycocalyx in invertebrate spermatozoa has already been demonstrated. Recently Yanagimachi et al. has determined the negative charges on sperm surfaces of mammalian spermatozoa including man, using colloidal iron hydroxide. No mention was made however of the outer surface coat as composed of substances other than those confering a negative charge. The purpose of this work was therefore to determine the presence of a glycocalyx in human spermatozoa using alcian blue and lanthanum staining.

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A High Resolution Cytochemical Study of Nuclear ATPase in Human Spermatozoa

Proceedings, annual meeting, Electron Microscopy Society of America ◽

10.1017/s0424820100116590 ◽

1975 ◽

Vol 33 ◽

pp. 594-595

Author(s):

A. Sosa ◽

L. Calzada

Keyword(s):

High Resolution ◽

Atpase Activity ◽

Nuclear Membrane ◽

Human Sperm ◽

Sperm Nucleus ◽

Human Spermatozoa ◽

Cytochemical Study ◽

Membrane Bound ◽

Sperm Nuclei ◽

Interchromatin Space

The dependence of nuclear metabolism on the function of the nuclear membrane is not well understood. Whether or not the function of the nuclear membrane is partial or totally responsible of the repressed template activity of human sperm nucleus has not at present been elucidated. One of the membrane-bound enzymatic activities which is concerned with the mechanisms whereby substances are thought to cross cell membranes is adenosintriphosphatase (ATPase). This prompted its characterization and distribution by high resolution photogrammetry on isolated human sperm nuclei. Isolated human spermatozoa nuclei were obtained as previously described. ATPase activity was demonstrated by the method of Wachstein and Meisel modified by Marchesi and Palade. ATPase activity was identified as dense and irregularly distributed granules confined to the internal leaflet of the nuclear membrane. Within the nucleus the appearance of the reaction product occurs as homogenous and dense precipitates in the interchromatin space.

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