scholarly journals A 38 h interval between hCG priming and oocyte retrieval increases in vivo and in vitro oocyte maturation rate in programmed IVM cycles

2008 ◽  
Vol 23 (9) ◽  
pp. 2010-2016 ◽  
Author(s):  
W.-Y. Son ◽  
J.-T. Chung ◽  
R.-C. Chian ◽  
B. Herrero ◽  
E. Demirtas ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Oocyte Retrieval ◽  
Maturation Rate ◽  
In Vitro Oocyte Maturation

scholarly journals Differences in transcriptomic profiles of human cumulus cells isolated from oocytes at GV, MI and MII stages after in vivo and in vitro oocyte maturation

2012 ◽  
Vol 27 (8) ◽  
pp. 2438-2447 ◽  
Author(s):  
Zamalou Gisèle Ouandaogo ◽  
Nelly Frydman ◽  
Laetitia Hesters ◽  
Said Assou ◽  
Delphine Haouzi ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Cumulus Cells ◽  
In Vitro Oocyte Maturation

Effects of caffeine on in vivo and in vitro oocyte maturation in mice

2007 ◽  
Vol 68 (4) ◽  
pp. 640-645 ◽  
Author(s):  
Yi-Liang Miao ◽  
Li-Hong Shi ◽  
Zi-Li Lei ◽  
Jun-Cheng Huang ◽  
Ji-Wen Yang ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
In Vitro Oocyte Maturation

6 EXPRESSION PROFILING OF SINGLE BOVINE EMBRYOS REVEALS SIGNIFICANT EFFECTS OF IN VITRO MATURATION, FERTILIZATION AND CULTURE

2006 ◽  
Vol 18 (2) ◽  
pp. 111
Author(s):  
S. L. Smith ◽  
L.-Y. Sung ◽  
R. Page ◽  
B. Henderson ◽  
F. Du ◽  
...  
Keyword(s):  
Gene Expression ◽  
Differentially Expressed Genes ◽  
Oocyte Maturation ◽  
Expression Profiling ◽  
Bovine Serum ◽  
In Vitro Maturation ◽  
Differentially Expressed ◽  
In Vitro Oocyte Maturation

Cattle and sheep embryos transferred after in vitro production are often afflicted by large offspring syndrome (LOS), which has been correlated with the presence of serum and/or cell co-culture. Previous research indicates that post-fertilization culture affects blastocyst quality and gene expression, and in vitro oocyte maturation and fertilization impact developmental competence. To dissect the effects of in vitro maturation, fertilization, and culture, we compared the expression profiles of single bovine blastocysts generated by: (1) in vitro maturation, fertilization and culture (IVF, n = 15); (2) in vivo maturation, in vivo fertilization, and in vitro culture (IVD, n = 14); and (3) in vivo maturation, fertilization, and development (AI, n = 14). For in vitro culture, the embryos were cultured for 2 days in CR1aa medium with bovine serum albumin (BSA) and then transferred to CR1aa with 10% fetal bovine serum (FBS) with cumulus cells until Day 7, at which time the embryos were vitrified. IVD zygotes were surgically collected from two superovulated Holstein donor cows 24 h post-insemination and cultured in the same system. To conduct expression profiling, total RNA was isolated from individual thawed embryos. The RNA was subjected to three rounds of amplification utilizing a previously adapted and validated T7 linear amplification protocol. Amplified RNA from each embryo and from a standard reference was indirectly labeled with Cy3 or Cy5 by dye swap and hybridized to a custom bovine cDNA microarray containing ~6300 unique genes. After Loess normalization, an ANOVA model (GeneSpring 6.1 and SAS 9.0) was used to identify differentially expressed genes. The P-values were adjusted for multiple comparisons using the false discovery rate approach, and a e2-fold differential criterion was applied. A subset of the differentially expressed genes was verified by real-time RT-PCR. The blastocyst rates for IVF and IVD embryos were 37% and 75%, respectively. There were 305, 365, and 200 genes differentially expressed between the AI and IVD, the IVF and IVD, and the AI and IVF comparisons, respectively. Interestingly, 44 differentially expressed genes were identified between the AI embryos and both the IVF and the IVD embryos, making these potential candidates for LOS. There were 61 genes differentially expressed between the IVF embryos and the AI and IVD embryos. The Gene Ontology categories 'RNA processing' and 'RNA binding' were over-represented among the genes that were down-regulated in the IVF embryos, indicating an effect of in vitro oocyte maturation/fertilization on embryonic gene expression. This work was supported by USDA grants to X.Y., H.A.L., and X.C.T.

Improvement of the in vitro growth and maturation of isolated mouse preantral follicles in the presence of repaglinide

2019 ◽  
Vol 9 (o3) ◽  
Author(s):  
Sura A. Awadh ◽  
Mehri Azadbakht ◽  
Faris N. A. Alhady
Keyword(s):  
Oocyte Maturation ◽  
Ovarian Follicle ◽  
Control Group ◽  
In Vitro Growth ◽  
Preantral Follicles ◽  
Maturation Rate ◽  
Reproductive Techniques ◽  
In Vitro Oocyte Maturation ◽  
Survival Density

The development of in vitro culture systems that result to preantral follicles growth and increasing of developmental competency of oocytes obtained from follicles has an important role in fertility preservation and assisted reproductive techniques. In this research, we evaluated the effect of repaglinide on in vitro growth and maturation of preantral follicles. Preantral follicles were isolated from 12-14 day-old female NMRI mice ovaries and cultured for 12 days cultured in α-MEM (Control), α-MEM supplemented with 1µM of repaglinide. Follicles examined for development on 1, 3, 6, 9, 12 days of culture. At the end of culture period after HCG administration in vitro oocyte maturation was assessed. Results showed that in vitro follicle growth, survival, density of granulosa cells and steroidogenic activity were higher than the control group (p less than 0.05). In vitro maturation rate in oocytes derived from follicles in the treatment group was higher than control group (p less than 0.05). Therefore the supplementation of the culture medium with repaglinide can improve the ovarian follicle survival, growth and subsequently in vitro oocyte maturation.

188 EFFECTS OF IN VITRO MATURATION ON GENE EXPRESSION IN RHESUS MONKEY OOCYTES

2009 ◽  
Vol 21 (1) ◽  
pp. 193
Author(s):  
Y. S. Lee ◽  
C. A. VandeVoort ◽  
K. E. Latham
Keyword(s):  
Gene Expression ◽  
Assisted Reproduction ◽  
Oocyte Maturation ◽  
Cell Interactions ◽  
Differentially Expressed ◽  
Primate Species ◽  
In Vitro Oocyte Maturation ◽  
Human Primate

Assisted reproduction technologies (ARTs) are achieving increasing prominence in reproductive medicine. With the increasing application of ARTs comes increased interest in optimizing efficiency while minimizing potential risks to the offspring. One area of assisted reproduction in which improvements are being sought is in vitro oocyte maturation. In vitro oocyte maturation (IVM) holds great promise as a tool for enhancing clinical treatment of infertility, enhancing availability of non-human primates for development of disease models, and facilitating endangered species preservation. However, IVM outcomes have remained significantly below success rates obtained using in vivo-matured (VVM) oocytes from humans and non-human primates. There is thus considerable interest in improving IVM. Key objectives toward achieving more efficient IVM will be to establish the molecular determinants of oocyte quality, identify specific biological processes or mechanisms that may be disrupted by ARTs, and identify specific modifications to procedures to eliminate these deficiencies. This study provides the first global comparison of mRNA expression profiles between in vitro- and in vivo-matured metaphase II stage oocytes in a non-human primate species. RNAs isolated from oocytes of each kind (IVM and VVM) were subjected to a 2-cycle labeling assay, and the labeled cRNAs were hybridized to Affymetrix rhesus macaque genome arrays (Affymetrix Inc., Santa Clara, CA, USA). To minimize false positive signals, only genes called present in at least 3 out of 4 biological replicates were used for significance analysis of microarray. Genes with significant differences among samples were identified at the 5% false discovery rate and were further selected on the basis of t-test (P < 0.05). We observed a small set of just 59 mRNAs that are differentially expressed between the 2 types of oocytes. Independent confirmation of gene expression differences was performed for 19 candidate genes using the quantitative RT-PCR. Gene functional classification analysis revealed that genes differentially expressed between IVM and VVM oocytes are related to cellular homeostasis, cell-cell interactions including growth factor and hormone stimulation and cell adhesion, and other functions such as mRNA stability and translation. Additionally, we observed in IVM oocytes overexpression of PLAGL1 and MEST, 2 maternally imprinted genes, indicating a possible interruption or loss of correct epigenetic programming. These results provide novel insight into the nature of oocyte-follicle cell interactions, the potential molecular and cellular consequences of altering these interactions, and the basis for compromised developmental competence following IVM procedures in a non-human primate model. The results also raise concerns about applying IVM clinically without addressing such developmental defects but indicate that these deficiencies may be overcome by further improvement in IVM culture systems. This study was supported by grants from the National Institutes of Health, National Centers for Research Resources (NCRR) RR15253 (KEL), RR000169 (CAV), and RR13439 (CAV).

Research Progress of In vitro Oocyte Maturation

2021 ◽  
Vol 0 (0) ◽  
pp. 0
Author(s):  
Xue-Hong Zhang ◽  
Yuan-Xue Jing ◽  
Yi-Qing Wang ◽  
Hong-Xing Li ◽  
Feng Yue ◽  
...  
Keyword(s):  
Oocyte Maturation ◽  
Research Progress ◽  
In Vitro Oocyte Maturation
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