scholarly journals First polar body morphology before ICSI is not related to embryo quality or pregnancy rate

2004 ◽  
Vol 19 (10) ◽  
pp. 2334-2339 ◽  
Author(s):  
P.M. Ciotti ◽  
L. Notarangelo ◽  
A.M. Morselli-Labate ◽  
V. Felletti ◽  
E. Porcu ◽  
...  
Keyword(s):  
Pregnancy Rate ◽  
Embryo Quality ◽  
Polar Body ◽  
Body Morphology ◽  
First Polar Body

Relationship between first polar body morphology before intracytoplasmic sperm injection and fertilization rate, cleavage rate, and embryo quality

2008 ◽  
Vol 104 (3) ◽  
pp. 226-229 ◽  
Author(s):  
Paula Andrea Navarro ◽  
Maria Medeiros de Araújo ◽  
Carlos Medeiros de Araújo ◽  
Marcelo Rocha ◽  
Rosana dos Reis ◽  
...  
Keyword(s):  
Intracytoplasmic Sperm Injection ◽  
Embryo Quality ◽  
Polar Body ◽  
Fertilization Rate ◽  
Cleavage Rate ◽  
Body Morphology ◽  
First Polar Body

First polar body morphology before ICSI is not related to fertilization rate, cleavage rate, or embryo quality

2008 ◽  
Vol 90 ◽  
pp. S221
Author(s):  
P.A.A.S. Navarro ◽  
R.M. Reis ◽  
M.C.P. Medeiros de Araújo ◽  
C.H. Medeiros de Araújo ◽  
M.G. Rocha ◽  
...  
Keyword(s):  
Embryo Quality ◽  
Polar Body ◽  
Fertilization Rate ◽  
Cleavage Rate ◽  
Body Morphology ◽  
First Polar Body

315 EFFICIENCY OF CHEMICAL OR ELECTRICAL ACTIVATION OF BOVINE OOCYTES

2006 ◽  
Vol 18 (2) ◽  
pp. 265
Author(s):  
M. P. Milazzotto ◽  
W. B. Feitosa ◽  
R. Simões ◽  
C. M. Mendes ◽  
M. E. O. A. Assumpção ◽  
...  
Keyword(s):  
Nuclear Transfer ◽  
Embryo Quality ◽  
Calcium Influx ◽  
Polar Body ◽  
Chemical Activation ◽  
Electrical Activation ◽  
Activation Process ◽  
Oocyte Activation ◽  
Blastocyst Formation ◽  
First Polar Body

Activation of in vitro matured oocytes is essential for the success of nuclear transfer embryo production. Oocyte activation is promoted by the release of intracellular calcium and influx of extracellular ions, and can be chemically induced by calcium ionophores such as A23187 (CA) or ionomycin (IO). Electrical stimulation (EL) is an essential stage in nuclear transfer protocols for the fusion of enucleated oocytes with the donor's cell nucleus. Moreover, EL can be used as an alternative method to induce calcium influx through the formation of pores in the plasma membrane. This work aimed to evaluate the effect of electrical pulse vs the use of different calcium ionophores (A23187 or ionomycin) as primary agents of bovine oocyte activation, with or without the addition of BSA, on the rate of blastocyst formation and blastocyst quality. BSA was used to quench the activation process after a 5-min exposure to CA or IO. Cumulus-oocyte complexes were matured in TCM-199 medium with FCS and hormones for 18 h at 38.5�C and 5% CO2 in air. After removal of cumulus cells, oocytes presenting the first polar body were selected and maintained in SOFaa medium to complete 24 h of maturation. They were then divided into five treatments groups 1-CA (CA 5 mM, 5 min); 2-CAB (CA 5 mM, 5 min; BSA, 5 min); 3-IO (IO 5 mM, 5 min); 4-IOB (IO 5 mM, 5 min; BSA, 5 min); and 5-EL (EL 1.5 kV/cm, 20 �s, 2 pulses). After treatments, oocytes were kept in 6-dimethylaminopurine for 3 h and cultured in SOFaa medium for 7 days at 38.5�C and 5% CO2 in air. Rates of cleavage and blastocyst were evaluated respectively on Days 2 and 7 of culture. To evaluate embryo quality, Hoechst 33342/propidium iodide staining was used. Data were evaluated by ANOVA and submitted to LSD test for embryo rates and t-test for embryo quality. Four replicates were carried out with a total of 89 oocytes per treatment. There was a difference (P < 0.05) in rate of development to blastocyst between treatments 1-CA (54.4%a), 3-IO (51.4%a), and 5-EL (54.5%a) compared with 4-IOB (18.3%b). Treatment 2-CAB (39.8%ab) did not show any difference from the others. There was no difference (P > 0.05) among treatments in total number of cells: 1-CA (63.1a), 2-CAB (57.2a), 3-IO (60.9a), 4-IOB (72.4a), and 5-EL (58.4a). However, there was a difference (P < 0.01) in the percentage of viable cells between treatments 1-CA (49.9%a), 2-CAB (45.8%a), 3-IO (64.9%a), and 4-IOB (50.9%a) in comparison to 5-EL (82.7%b). In conclusion, BSA, when associated with IO, had a negative effect on embryonic developmental rates. The different calcium ionophores used and the BSA did not improve embryo quality. Although there were no significant differences between electrical and chemical activation on the rate of blastocyst formation, it is important to point out that higher quality embryos were achieved by using electrical activation. This work was supported by FAPESP 03/00156-9.

Elective transfer of embryos selected on the basis of first polar body morphology is associated with increased rates of implantation and pregnancy

1999 ◽  
Vol 72 (4) ◽  
pp. 599-603 ◽  
Author(s):  
Thomas Ebner ◽  
Marianne Moser ◽  
Cemil Yaman ◽  
Oscar Feichtinger ◽  
Johannes Hartl ◽  
...  
Keyword(s):  
Polar Body ◽  
Body Morphology ◽  
First Polar Body

scholarly journals Does first polar body morphology predict oocyte performance during ICSI treatment?

2009 ◽  
Vol 26 (11-12) ◽  
pp. 561-567 ◽  
Author(s):  
Johnny S. Younis ◽  
Orit Radin ◽  
Ido Izhaki ◽  
Moshe Ben-Ami
Keyword(s):  
Polar Body ◽  
Body Morphology ◽  
First Polar Body

32 PREGNANCY OF EQUINE CLONED EMBRYOS MICROINJECTED WITH PLURIPOTENCY INDUCING GENES (Oct4, Sox2, c-Myc, K1f4)

2014 ◽  
Vol 26 (1) ◽  
pp. 130
Author(s):  
R. Olivera ◽  
R. Jordan ◽  
C. Alvarez ◽  
M. Radrizzani ◽  
G. Vichera
Keyword(s):  
Pregnancy Rate ◽  
Electric Pulse ◽  
Polar Body ◽  
Donor Cell ◽  
Cumulus Cells ◽  
Pregnancy Rates ◽  
Control Group ◽  
Blastocyst Development ◽  
Donor Nucleus ◽  
First Polar Body

Animal cloning is a high impact tool for scientific and economical production, but still with inefficient results. The efficiency of the cloning process depends on the state of differentiation of the donor cell. An adult equine somatic cell can be differentiated to a pluripotent stem cell (iPSC) inducing the expression of certain transcription factors (Oct4, Sox2, c-Myc, and K1f4; Breton et al. 2013). The objective of this work was to assess the effect of the intracytoplasmic injection of pluripotency inducing genes on embryo development and pregnancy rates of equine cloned embryos. Cumulus–oocyte complexes (COC) were obtained from slaughterhouse ovaries. Oocyte collection and maturation procedure were performed as described by Lagutina et al. (2007). After the removal of cumulus cells, oocytes showing first polar body were microinjected with a mixture 1/3 of plasmids/liposomes (Mi group). The plasmid used was the pEP4-E02s-EM2k, which encodes the human genes Oct4, Sox2, Myc, and K1f4. The DNA concentration was adjusted to 0.5 μg mL–1. Microinjected oocytes were enucleated using the zona free method. Adult male skin fibroblasts from the same animal were used as donor nucleus cells. These fibroblasts were attached to the ooplasts with phytohemagglutinin and then fused with an electric pulse. Activation was performed using 8.7 mM ionomycin for 4 min, followed by culture for 4 h in a combination of 1 mM 6-DMAP and 5 mg mL–1 cycloheximide. Zona free reconstructed embryos (ZFRE) were cultured for 7 to 8 days in DMEM-F12 in the well of the well (WOW) system, aggregating 3 embryos per well. A control group (CC group) of not microinjected embryos was included. Cleavage and blastocyst development was assessed at Days 2 and 7, respectively. Transcervical transfer of 49 Day 7 to 8 blastocysts was performed 6 days after ovulation. The mares received 2 blastocysts per transfer. Pregnancy was diagnosed by transrectal ultrasonography 15 days after ovulation. Cleavage and blastocyst rates were analysed by Chi-squared test and pregnancy rate by Fisher test (P < 0.05). Cleavage was 92.1% (n = 58/63) for the Mi group and 90.4% (n = 868/960) for the CC group. Blastocyst rate was statistically higher per well, 28.6% (n = 6/21) v. 13.4% (n = 43/320) but not per oocyte, 9.5% (n = 6/63) v. 4.5% (n = 43/960), for the Mi and CC groups, respectively. Pregnancy rate was 17% (n = 1/6) for the Mi group and 7% (n = 3/43) for the CC group. No twin pregnancies were found and all the pregnancies are still ongoing. The higher blastocyst rates obtained with the embryos microinjected with pluripotency inducing genes compared with the control group showed an improvement in embryo quality. In conclusion, the data presented indicate that the intracytoplasmic microinjection of pluripotency inducing genes in equine zona free cloned embryos improved blastocyst rates on a per well basis and showed a tendency to improve the pregnancy rates. The expression of the Oct4, Sox2, c-Myc, and K1f4 genes could be probably generating better reprogrammed donor nucleus compared with adult differentiated cells used in conventional cloning.

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