Development of automated annotation software for human embryo morphokinetics

2020 ◽  
Vol 35 (3) ◽  
pp. 557-564 ◽  
Author(s):  
M Feyeux ◽  
A Reignier ◽  
M Mocaer ◽  
J Lammers ◽  
D Meistermann ◽  
...  
Keyword(s):  
Human Embryo ◽  
Embryo Quality ◽  
Selection Criterion ◽  
Time Lapse ◽  
Manual Annotation ◽  
Annotation Tool ◽  
High Throughput Analysis ◽  
Technical Limitation ◽  
Automated Annotation ◽  
Morphokinetic Parameters

Abstract STUDY QUESTION Is it possible to develop an automated annotation tool for human embryo development in time-lapse devices based on image analysis? SUMMARY ANSWER We developed and validated an automated software for the annotation of human embryo morphokinetic parameters, having a good concordance with expert manual annotation on 701 time-lapse videos. WHAT IS KNOWN ALREADY Morphokinetic parameters obtained with time-lapse devices are increasingly used for the assessment of human embryo quality. However, their annotation is time-consuming and can be slightly operator-dependent, highlighting the need to develop fully automated approaches. STUDY DESIGN, SIZE, DURATION This monocentric study was conducted on 701 videos originating from 584 couples undergoing IVF with embryo culture in a time-lapse device. The only selection criterion was that the duration of the video must be over 60 h. PARTICIPANTS/MATERIALS, SETTING, METHODS An automated morphokinetic annotation tool was developed based on gray level coefficient of variation and detection of the thickness of the zona pellucida. The detection of cellular events obtained with the automated tool was compared with those obtained manually by trained experts in clinical settings. MAIN RESULTS AND THE ROLE OF CHANCE Although some differences were found when embryos were considered individually, we found an overall concordance between automated and manual annotation of human embryo morphokinetics from fertilization to expanded blastocyst stage (r2 = 0.92). LIMITATIONS, REASONS FOR CAUTION These results should undergo multicentric external evaluation in order to test the overall performance of the annotation tool. Getting access to the export of 3D videos would enhance the quality of the correlation with the same algorithm and its extension to the 3D regions of interest. A technical limitation of our work lies within the duration of the video. The more embryo stages the video contains, the more information the script has to identify them correctly. WIDER IMPLICATIONS OF THE FINDINGS Our system paves the way for high-throughput analysis of multicentric morphokinetic databases, providing new insights into the clinical value of morphokinetics as a predictor of embryo quality and implantation. STUDY FUNDING/COMPETING INTEREST(S) This study was partly funded by Finox-Gedeon Richter Forward Grant 2016 and NeXT (ANR-16-IDEX-0007). We have no conflict of interests to declare. TRIAL REGISTRATION NUMBER N/A

scholarly journals Development of a robust automated tool for the annotation of embryo morphokinetic parameters

2018 ◽  
Author(s):  
M Feyeux ◽  
A Reignier ◽  
M Mocaer ◽  
J Lammers ◽  
D Meistermann ◽  
...  
Keyword(s):  
Human Embryo ◽  
Large Scale ◽  
Embryo Quality ◽  
Time Lapse ◽  
Preimplantation Embryos ◽  
Manual Annotation ◽  
Annotation Tool ◽  
Automated Annotation ◽  
Morphokinetic Parameters ◽  
Automated Tool

AbstractStudy QuestionIs it possible to automatically annotate human embryo development in time-lapse devices, with results comparable to manual annotation?Summary AnswerWe developed an automated tool for the annotation of embryo morphokinetic parameters having a high concordance with expert manual annotation in a large scale-study.What is Known AlreadyMorphokinetic parameters obtained with time-lapse devices are increasingly used for human embryo quality assessment. However, their annotation is timeconsuming and can be operator-dependent, highlighting the need of developing automated approaches.Study Design, Size, DurationThis monocentric pilot study was conducted using 701 blastocysts originating from 584 couples undergoing IVF with embryo culture in a time-lapse device and on 4 mouse embryos.Participants/Materials, Setting, MethodsAn automated annotation tool was developed based on grey level coefficient of variation and detection of the thickness of the zona pellucida. The timings of cellular events obtained with the automated tool were compared with those obtained manually by 2 expert embryologists. The same procedure was applied on 4 mouse preimplantation embryos obtained with a different device in a different setting.Main Results and the Role of ChanceAlthough some differences were found when embryos were considered individually, we found an overall excellent concordance between automated and manual annotation of human embryo morphokinetics from fertilization to expanded blastocyst stage (r2=0.94). Moreover, the automated annotation tool gave promising results across species (human, mice).Limitations, Reasons for CautionThese results should undergo multi-centric external evaluation in order to test the overall performance of the annotation tool.Wider Implications of the FindingsOur system performs significantly better than the ones reported in the literature and on a bigger cohort, paving the way for high-throughput analysis of multicentric morphokinetic databases, providing new insights into the clinical value of morphokinetics as predictor of embryo quality and implantation.Study Funding/Competing Interest(s)This study was partly funded by Finox Forward Grant 2016.Trial Registration NumberNA

scholarly journals Analysis of Morphokinetic Parameters of Feline Embryos Using a Time-Lapse System

Animals ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 748
Author(s):  
Joanna Kochan ◽  
Agnieszka Nowak ◽  
Barbara Kij ◽  
Sylwia Prochowska ◽  
Wojciech Niżański
Keyword(s):  
Embryo Development ◽  
Embryo Quality ◽  
Time Lapse ◽  
Blastocyst Stage ◽  
Cavity Formation ◽  
Non Invasive ◽  
Morphokinetic Parameters ◽  
Morphological Defects ◽  
Development In Vitro

The aim of this study was to analyze the morphokinetic parameters of feline embryos using a time lapse system. Oocytes matured in vitro were fertilized (IVF) and in vitro cultured in a time lapse-system (Primo Vision®, Gothenburg, Sweden). The first cell division of embryos occurred between 17 h post insemination (hpi) and 38 hpi, with the highest proportion of embryos (46%) cleaving between 21 and 24 hpi. The timing of the first cleavage significantly affected further embryo development, with the highest development occurring in embryos that cleaved at 21–22 hpi. Embryos that cleaved very early (17–18 hpi) developed poorly to the blastocyst stage (2%) and none of the embryos that cleaved later than 27 hpi were able to reach the blastocyst stage. Morphological defects were observed in 48% of the embryos. There were no statistically significant differences between the timing intervals of the first cleavage division and the frequency of morphological defects in embryos. Multiple (MUL) morphological defects were detected in more than half (56%) of the abnormal embryos. The most frequent single morphological defects were cytoplasmic fragmentation (FR) (8%) and blastomere asymmetry (AS) (6%). Direct cleavage (DC) from 1–3 or 3–5 blastomeres, reverse cleavage (RC) and vacuoles were rarely observed (2–3%). The timing of blastocyst cavity formation is a very good indicator of embryo quality. In our study, blastocyst cavity formation occurred between 127–167 hpi, with the highest frequency of hatching observed in blastocysts that cavitated between 142–150 hpi. Blastocysts in which cavitation began after 161 h did not hatch. In conclusion, the timing of the first and second cleavage divisions, the timing of blastocyst cavity formation and morphological anomalies can all be used as early and non-invasive indicators of cat embryo development in vitro.

scholarly journals Embryo Morphokinetic Parameters Evaluated Using a Time-Lapse Embryo Monitoring System Can Predict the Embryo Developmental Potential in ICSI Cycles

2021 ◽  
Author(s):  
Qina He ◽  
Guidong Yao ◽  
Jiahuan He ◽  
Guang Yang ◽  
Ziwen Xu ◽  
...  
Keyword(s):  
Monitoring System ◽  
Embryo Quality ◽  
Morphological Characteristics ◽  
Time Lapse ◽  
Reproductive Technologies ◽  
Embryo Morphology ◽  
High Quality ◽  
Developmental Potential ◽  
Morphokinetic Parameters ◽  
The Mean

Abstract At present, embryo morphology assessment based on the observation of the embryonic morphological characteristics at several specific time points has been mainly used for selecting the high-quality embryo. However, we all know that embryo development is a dynamic process. Many research results on the correlation between the embryo morphokinetic parameters and embryo quality and development potential were inconsistent. With the help of time-lapse imaging, the development processes and outcomes of a total of 365 embryos were cultured and analyzed in this study. The results showed that the mean tPNf and t2 of the high-quality embryo were significantly shorter than the low-quality embryo; the mean t2PBe and tPNa of the high-quality embryo from the implantation group were significantly shorter than those from the non-implantation group. In addition, based on the quartile grouping of each morphokinetic parameter, the embryos that had 21.15≤tPNf≤25.30 value were significantly higher in embryo quality when compared with the embryos that had the tPNf values outside the range on Days 3. Similarly, the embryos that had values of t2≤25.60 were significantly higher in embryo quality than those with outside the range values on Days 3. Thus, we demonstrated that the morphokinetic parameter evaluated using a time-lapse embryo monitoring system can predict the embryo quality, and be benefit for the selection of the high-quality embryos and improvement for the implantation success of the patient in assisted reproductive technologies.

scholarly journals Morphokinetic parameters using time-lapse technology and day 5 embryo quality: a prospective cohort study

2015 ◽  
Vol 32 (7) ◽  
pp. 1151-1160 ◽  
Author(s):  
Ashleigh Storr ◽  
Christos A. Venetis ◽  
Simon Cooke ◽  
Daisy Susetio ◽  
Suha Kilani ◽  
...  
Keyword(s):  
Cohort Study ◽  
Prospective Cohort Study ◽  
Prospective Cohort ◽  
Embryo Quality ◽  
Time Lapse ◽  
Morphokinetic Parameters

scholarly journals Time-lapse imaging of cleavage divisions in embryo quality assessment

Reproduction ◽  
2017 ◽  
Vol 154 (2) ◽  
pp. R37-R53 ◽  
Author(s):  
Robert Milewski ◽  
Anna Ajduk
Keyword(s):  
Embryo Quality ◽  
Biological Significance ◽  
Time Lapse ◽  
Reproductive Outcome ◽  
Future Perspectives ◽  
Morphokinetic Parameters ◽  
Current Review ◽  
Predictive Algorithms ◽  
Time Lapse Imaging

In vitrofertilization (IVF) is one of the most important procedures for treating infertility. As several embryos are usually produced in a single IVF cycle, it is crucial to select only the most viable ones for transfer to the patient. Morphokinetics, i.e. analysis of the dynamics of cleavage divisions and processes such as compaction and cavitation, has provided both biologists and clinicians with a new set of data regarding embryonic behaviour during preimplantation development and its association with embryo quality. In the current review, we focus on biological significance of morphokinetic parameters and show how they can be used to predict a reproductive outcome. We also explain the statistics behind the predictive algorithms and discuss the future perspectives of morphokinetics.

Sperm DNA fragmentation measured by sperm chromatin dispersion impacts morphokinetic parameters, fertilization rate and blastocyst quality in ICSI treatments

Zygote ◽  
2021 ◽  
pp. 1-8
Author(s):  
Shikai Wang ◽  
Weihong Tan ◽  
Yueyue Huang ◽  
Xianbao Mao ◽  
Zhengda Li ◽  
...  
Keyword(s):  
Dna Fragmentation ◽  
Embryo Quality ◽  
Fertilization Rate ◽  
Sperm Chromatin ◽  
Sperm Dna Fragmentation ◽  
Blastocyst Formation ◽  
High Quality ◽  
Morphokinetic Parameters ◽  
Sperm Dna ◽  
Blastocyst Quality

Summary To determine the effects of sperm DNA fragmentation (SDF) on embryo morphokinetic parameters, cleavage patterns and embryo quality, this retrospective study analyzed 151 intracytoplasmic sperm injection (ICSI) cycles (1152 embryos collected) between November 2016 and June 2019. SDF was assessed using sperm chromatin dispersion. The cycles were divided into two groups based on the SDF rate: SDF < 15% (n = 114) and SDF ≥ 15% (n = 37). The embryo morphokinetic parameters, cleavage patterns, and embryo quality were compared between the two groups. The morphokinetic parameters tPNf, t2, t3, t4, t5, t6, and t8 were achieved significantly earlier in the SDF < 15% group compared with in the SDF ≥ 15% group. The fertilization and 2PN rates seemed to be significantly higher in the SDF < 15% group compared with in the SDF ≥ 15% group, while the abnormal cleavage rates were similar. However, a significantly higher rate of chaotic cleavage (CC) was observed in the SDF ≥ 15% group. The D3 high-quality embryo and available embryo rates were similar between the two groups. The blastocyst formation, high-quality blastocyst, and available blastocyst rates in the SDF < 15% group were significantly higher than those in the SDF ≥ 15% group. With an increase in SDF level, the chemical pregnancy, clinical pregnancy and implantation rates tended to decrease, while the miscarriage rate increased. This study demonstrated that SDF ≥ 15% reduces the fertilization rate of ICSI cycles and affects certain morphokinetic parameters. A higher SDF level can also induce a higher rate of CC, with subsequent decreases in the blastocyst formation rate and blastocyst quality.

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