scholarly journals Detection of human sperm acrosome reaction: comparison between methods using double staining, Pisum sativum agglutinin, concanavalin A and transmission electron microscopy

1997 ◽  
Vol 12 (4) ◽  
pp. 714-721 ◽  
Author(s):  
F. M. Kohn ◽  
S. R. Mack ◽  
W. B. Schill ◽  
L. J. Zaneveld
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Pisum Sativum ◽  
Concanavalin A ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Double Staining ◽  
Transmission Electron ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

Assessment of the human sperm acrosome reaction using concanavalin a lectin

1990 ◽  
Vol 25 (3) ◽  
pp. 247-257 ◽  
Author(s):  
Carol A. Holden ◽  
Ross V. Hyne ◽  
A. H. Sathananthan ◽  
Alan O. Trounson
Keyword(s):  
Concanavalin A ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Synchrotron Radiation Spectroscopy and Transmission Electron Microscopy Techniques to Evaluate TiO2 NPs Incorporation, Speciation, and Impact on Root Cells Ultrastructure of Pisum sativum L. Plants

Nanomaterials ◽  
2021 ◽  
Vol 11 (4) ◽  
pp. 921
Author(s):  
Simonetta Muccifora ◽  
Hiram Castillo-Michel ◽  
Francesco Barbieri ◽  
Lorenza Bellani ◽  
Monica Ruffini Castiglione ◽  
...  
Keyword(s):  
Electron Microscopy ◽  
Transmission Electron Microscopy ◽  
Synchrotron Radiation ◽  
Pisum Sativum ◽  
Emission Spectrometry ◽  
Root Cells ◽  
Tio2 Nps ◽  
X Ray ◽  
Pisum Sativum L ◽  
Transmission Electron

Biosolids (Bs) for use in agriculture are an important way for introducing and transferring TiO2 nanoparticles (NPs) to plants and food chain. Roots of Pisum sativum L. plants grown in Bs-amended soils spiked with TiO2 800 mg/kg as rutile NPs, anatase NPs, mixture of both NPs and submicron particles (SMPs) were investigated by Transmission Electron Microscopy (TEM), synchrotron radiation based micro X-ray Fluorescence and micro X-ray Absorption Near-Edge Structure (µXRF/µXANES) and Inductively Coupled Plasma Optical Emission Spectrometry (ICP-OES). TEM analysis showed damages in cells ultrastructure of all treated samples, although a more evident effect was observed with single anatase or rutile NPs treatments. Micro-XRF and TEM evidenced the presence of nano and SMPs mainly in the cortex cells near the rhizodermis. Micro-XRF/micro-XANES analysis revealed anatase, rutile, and ilmenite as the main TiO2 polymorphs in the original soil and Bs, and the preferential anatase uptake by the roots. For all treatments Ti concentration in the roots increased by 38–56%, however plants translocation factor (TF) increased mostly with NPs treatment (261–315%) and less with SMPs (about 85%), with respect to control. In addition, all samples showed a limited transfer of TiO2 to the shoots (very low TF value). These findings evidenced a potential toxicity of TiO2 NPs present in Bs and accumulating in soil, suggesting the necessity of appropriate regulations for the occurrence of NPs in Bs used in agriculture.

scholarly journals Effect of acetylcholine on the human sperm acrosome reaction.

1996 ◽  
Vol 71 ◽  
pp. 257
Author(s):  
Akira Nozawa. ◽  
Koji Ogasawara. ◽  
Satoru Kaneko. ◽  
Koichi Takeuchi ◽  
Takao Hashimoto
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

Are capacitation or calcium ion influx required for the human sperm acrosome reaction?

1994 ◽  
Vol 62 (6) ◽  
pp. 1255-1261 ◽  
Author(s):  
Peter Bielfeld ◽  
Robert A. Anderson ◽  
Steven R. Mack ◽  
Christopher J. De Jonge ◽  
Lourens J.D. Zaneveld
Keyword(s):  
Calcium Ion ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Ion Influx ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals Progesterone Triggers a Wave of Increased Free Calcium during the Human Sperm Acrosome Reaction

1997 ◽  
Vol 182 (1) ◽  
pp. 67-75 ◽  
Author(s):  
Stanley Meizel ◽  
Kenneth O. Turner ◽  
Richard Nuccitelli
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Free Calcium ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

Dynamics of human sperm acrosome reaction: relation with in vitro fertilization

1991 ◽  
Vol 55 (5) ◽  
pp. 994-999 ◽  
Author(s):  
Patrick Fénichel ◽  
Michèle Donzeau ◽  
Dariush Farahifar ◽  
Bernard Basteris ◽  
Noël Ayraud ◽  
...  
Keyword(s):  
In Vitro Fertilization ◽  
Acrosome Reaction ◽  
Human Sperm ◽  
Vitro Fertilization ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

scholarly journals INFLUENCE OF PROGESTERONE AND GABA A RECEPTOR ON CALCIUM MOBILIZATION DURING HUMAN SPERM ACROSOME REACTION

1999 ◽  
Vol 42 (3) ◽  
pp. 185-191 ◽  
Author(s):  
Y. KURODA ◽  
S KANEKO ◽  
Y YOSHIMURA ◽  
S NOZAWA ◽  
K. MIKOSHIBA
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Calcium Mobilization ◽  
Gaba A ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome

Steroid induced exocytosis: The human sperm acrosome reaction

1989 ◽  
Vol 160 (2) ◽  
pp. 828-833 ◽  
Author(s):  
Richard A. Osman ◽  
Matthew L. Andria ◽  
A. Daniel Jones ◽  
Stanley Meizel
Keyword(s):  
Acrosome Reaction ◽  
Human Sperm ◽  
Sperm Acrosome Reaction ◽  
Sperm Acrosome
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