scholarly journals Risk for nicotine dependence and lung cancer is conferred by mRNA expression levels and amino acid change in CHRNA5

2009 ◽  
Vol 18 (16) ◽  
pp. 3125-3135 ◽  
Author(s):  
Jen C. Wang ◽  
Carlos Cruchaga ◽  
Nancy L. Saccone ◽  
Sarah Bertelsen ◽  
Pengyuan Liu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Amino Acid ◽  
Mrna Expression ◽  
Nicotine Dependence ◽  
Amino Acid Change ◽  
Expression Levels ◽  
Mrna Expression Levels

scholarly journals Comprehensive analysis of inhibitor of differentiation/DNA-binding gene family in lung cancer using bioinformatics methods

2020 ◽  
Vol 40 (2) ◽  
Author(s):  
Suming Xu ◽  
Yaoqin Wang ◽  
Yanhong Li ◽  
Lei Zhang ◽  
Chunfang Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Transcription Factor ◽  
Dna Binding ◽  
Mrna Expression ◽  
Family Members ◽  
Enrichment Analysis ◽  
Functional Enrichment ◽  
Genetic Mutations ◽  
Expression Levels ◽  
Mrna Expression Levels

Abstract The inhibitor of differentiation/DNA-binding (ID) is a member of the helix–loop–helix (HLH) transcription factor family, and plays a role in tumorigenesis, invasiveness and angiogenesis. The aims were to investigate the expression patterns and prognostic values of individual ID family members in lung cancer, and the potential functional roles. The expression levels of ID family were assessed using the Oncomine online database and GEPIA database. Furthermore, the prognostic value of ID family members was evaluated using the Kaplan–Meier plotter database. The genetic mutations of ID family members were investigated using the cBioPortal database. Moreover, enrichment analysis was performed using STRING database and Funrich software. It was found that all the ID family members were significantly down-regulated in lung cancer. Prognostic results indicated that low mRNA expression levels of ID1 or increased mRNA expression levels of ID2/3/4 were associated with improved overall survival, first progression and post progression survival. Additionally, genetic mutations of ID family members were identified in lung cancer, and it was suggested that amplification and deep deletion were the main mutation types. Furthermore, functional enrichment analysis results suggested that ID1/2/4 were significantly enriched in ‘regulation of nucleobase, nucleoside, nucleotide and nucleic acid metabolism’ for biological process, ‘transcription factor activity’ for molecular function and ‘HLH domain’ for protein domain. However, it was found that ID3 was not enriched in the above functions. The aberrant expression of ID family members may affect the occurrence and prognosis of lung cancer, and may be related to cell metabolism and transcriptional regulation.

scholarly journals HPV16 E6/E7 upregulate hTERC mRNA and gene amplification levels by relieving the effect of LKB1 on Sp1 phosphorylation in lung cancer cells

2020 ◽  
Vol 12 ◽  
pp. 175883592091756
Author(s):  
Jing-Hua Yang ◽  
Ming-Zhe Wu ◽  
Xu-Bo Wang ◽  
Shiyu Wang ◽  
Xue-Shan Qiu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Mrna Expression ◽  
Gene Amplification ◽  
Genetic Manipulation ◽  
Mrna Levels ◽  
Promoter Regions ◽  
Hpv16 E6 ◽  
Expression Levels ◽  
Malignant Group ◽  
Mrna Expression Levels

Background: There is an immediate need for research on the mechanism underlying telomerase activation and overexpression. Materials & Methods: A total of 174 patients with lung cancer ( n = 106) and benign lung disease ( n = 68) were recruited for the current study. The mRNA expression levels of E6, E7, LKB1, Sp1, and hTERC in brushing cells were detected by quantitative reverse transcriptase polymerase chain reaction (qRT-PCR), and hTERC amplification was also detected by fluorescence in situ hybridization (FISH). To investigate the potential mechanism, bidirectional genetic manipulation was performed in well-established lung cancer cell lines. Results: Our results indicated that the mRNA expression levels of E6, E7, Sp1, and hTERC and the amplification level of hTERC were significantly increased in the malignant group compared with those of the benign group ( p < 0.01). Conversely, the mRNA expression level of LKB1 was significantly decreased in the malignant group ( p < 0.01). The correlation between E6, E7, Sp1, and hTERC expression was positive but was negative with LKB1 ( p < 0.01). Our results also showed that HPV16 E6/E7 downregulated the expression of LKB1 at both the protein and mRNA levels. The loss of LKB1 upregulated Sp1 expression, and also promoted Sp1 activity. Sp1 further upregulated hTERC at the mRNA and gene amplification levels. Thus, we proposed a HPV–LKB1–Sp1–hTERC axis of E6/E7 upregulation of hTERC expression. Conclusion: We demonstrated for the first time that E6 and E7 promoted hTERC mRNA expression and the amplification of hTERC by relieving the effect of LKB1 on the phosphorylation of Sp1. Sp1 further activated hTERC by directly binding to the promoter regions of hTERC.

Tradeoff between reproduction and resistance evolution to Bt-toxin in Helicoverpa armigera: regulated by vitellogenin gene expression

2014 ◽  
Vol 104 (4) ◽  
pp. 444-452 ◽  
Author(s):  
W.N. Zhang ◽  
H.J. Xiao ◽  
G.M. Liang ◽  
Y.Y. Guo ◽  
K.M. Wu
Keyword(s):  
Gene Expression ◽  
Amino Acid ◽  
Mrna Expression ◽  
Resistant Strain ◽  
Artificial Diet ◽  
Expression Levels ◽  
Bt Toxin ◽  
Resistance Evolution ◽  
Helicoverpa Armígera ◽  
Mrna Expression Levels

AbstractEvolution of resistance to insecticides usually has fitness tradeoffs associated with adaptation to the stress. The basic regulation mechanism of tradeoff between reproduction and resistance evolution to Bacillus thuringiensis (Bt) toxin in the cotton bollworm, Helicoverpa armigera (Ha), based on the vitellogenin (Vg) gene expression was analyzed here. The full-length cDNA of the Vg gene HaVg (JX504706) was cloned and identified. HaVg has 5704 base pairs (bp) with an open reading frame (ORF) of 5265 bp, which encoded 1756 amino acid protein with a predicted molecular mass of 197.28 kDa and a proposed isoelectric point of 8.74. Sequence alignment analysis indicated that the amino acid sequence of HaVg contained all of the conserved domains detected in the Vgs of the other insects and had a high similarity with the Vgs of the Lepidoptera insects, especially Noctuidae. The resistance level to Cry1Ac Bt toxin and relative HaVg mRNA expression levels among the following four groups: Cry1Ac-susceptible strain (96S), Cry1Ac-resistant strain fed on artificial diet with Bt toxin for 135 generations (BtR stands for the Cry1Ac Bt resistance), progeny of the Cry1Ac-resistant strain with a non-Bt-toxin artificial diet for 38 generations (CK1) and the direct descendants of the 135th-generation resistant larvae which were fed on an artificial diet without the Cry1Ac protein (CK2) were analyzed. Compared with the 96S strain, the resistance ratios of the BtR strain, the CK1 strain and the CK2 strain were 2917.15-, 2.15- and 2037.67-fold, respectively. The maximum relative HaVg mRNA expression levels of the BtR strain were approximately 50% less than that of the 96S strain, and the coming of maximum expression was delayed for approximately 4 days. The overall trend of the HaVg mRNA expression levels in the CK1 strain was similar to that in the 96S strain, and the overall trend of the HaVg mRNA expression levels in the CK2 strain was similar to that in the BtR strain. Our results suggest that the changes in reproduction due to the Bt-toxin resistance evolution in the BtR strain may be regulated by the Vg gene expression. The down-regulation of HaVg at the early stages resulted in a period of delayed reproduction and decreased fecundity in the BtR strain. This performance disappeared when the Bt-toxin selection pressure was lost.

scholarly journals Correlations of mRNA Levels among Efflux Transporters, Transcriptional Regulators, and Scaffold Proteins in Non-Small-Cell Lung Cancer

2021 ◽  
Vol 2021 ◽  
pp. 1-6
Author(s):  
Xieyi Zhang ◽  
Wangyang Liu ◽  
Kazue Edaki ◽  
Yuta Nakazawa ◽  
Hiroki Kamioka ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Multidrug Resistance ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Transcriptional Regulators ◽  
Scaffold Proteins ◽  
Lung Cancer Cells ◽  
Efflux Transporters ◽  
Expression Levels ◽  
Mrna Expression Levels

Multidrug resistance (MDR) due to enhanced drug efflux activity of tumor cells can severely impact the efficacy of antitumor therapies. We recently showed that increased activity of the efflux transporter P-glycoprotein (P-gp) associated with activation of Snail transcriptional regulators may be mediated mainly by moesin in lung cancer cells. Here, we aimed to systematically evaluate the relationships among mRNA expression levels of efflux transporters (P-gp, breast cancer resistance protein (BCRP), and multidrug resistance-associated protein 2 (MRP2)), scaffold proteins (ezrin (Ezr), radixin (Rdx), and moesin (Msn); ERM proteins), and SNAI family members (Snail, Slug, and Smac) in clinical lung cancer and noncancer samples. We found high correlations between relative (cancer/noncancer) mRNA expression levels of Snail and Msn, Msn and P-gp, Slug and MRP2, and Smuc and BCRP. These findings support our previous conclusion that Snail regulates P-gp activity via Msn and further suggest that Slug and Smuc may contribute to the functional regulation of MRP2 and BCRP, respectively, in lung cancer cells. This trial is registered with UMIN000023923.

ERCC1, RRM1 and BRCA1 mRNA expression levels and clinical outcome of advanced non-small cell lung cancer

2010 ◽  
Vol 28 (4) ◽  
pp. 1411-1417 ◽  
Author(s):  
Chunxia Su ◽  
Songwen Zhou ◽  
Ling Zhang ◽  
Shengxiang Ren ◽  
Jianfang Xu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Clinical Outcome ◽  
Small Cell Lung Cancer ◽  
Mrna Expression ◽  
Cell Lung Cancer ◽  
Small Cell ◽  
Small Cell Lung ◽  
Expression Levels ◽  
Brca1 Mrna ◽  
Mrna Expression Levels

scholarly journals Effects of Dietary Valine Levels on Production Performance, Egg Quality, Antioxidant Capacity, Immunity, and Intestinal Amino Acid Absorption of Laying Hens during the Peak Lay Period

Animals ◽  
2021 ◽  
Vol 11 (7) ◽  
pp. 1972
Author(s):  
Huafeng Jian ◽  
Sasa Miao ◽  
Yating Liu ◽  
Huaiyu Li ◽  
Wenting Zhou ◽  
...  
Keyword(s):  
Amino Acid ◽  
Mrna Expression ◽  
Laying Hens ◽  
Egg Quality ◽  
Production Performance ◽  
Serum Free ◽  
Expression Levels ◽  
Acid Absorption ◽  
Amino Acid Absorption ◽  
Mrna Expression Levels

The present study aimed to assess the impact of dietary valine levels on layer production performance, egg quality, immunity, and intestinal amino acid absorption of laying hens during the peak lay period. For this purpose, a total of 960 33-week-old Fengda No.1 laying hens were randomly divided into five experimental groups and fed with valine at the following different levels in a feeding trial that lasted 8 weeks: 0.59, 0.64, 0.69, 0.74, and 0.79%, respectively. Productive performances were recorded throughout the whole rearing cycle and the egg quality, serum indexes, and small intestine transporters expression were assessed at the end of the experiment after slaughter (41 weeks) on 12 hens per group. Statistical analysis was conducted by one-way ANOVA followed by LSD multiple comparison tests with SPSS 20.0 (SPSS, Chicago, IL, USA). The linear and quadratic effects were tested by SPSS 20.0. Egg mass, laying rate, broken egg rate, and feed conversion ratio were significantly improved with increasing dietary valine levels. However, the egg weight, eggshell thickness, albumen height, Haugh unit, and egg yolk color were significantly decreased with increasing dietary valine levels. Serum catalase (CAT), immunoglobulin A (IgA) and IgM levels, and malondialdehyde (MDA) levels were negative responses to valine-treated laying hens. Dietary supplemented valine enhanced the trypsin activity of duodenum chime and promoted the mRNA expression levels of ATB0,+, and LAT4 in the jejunum and corresponding serum free Ile, Lys, Phe, Val, and Tyr level. However, valine treatment significantly downregulated the mRNA expression levels of PePT1, B0AT1, LAT1, and SNAT2 in the small intestines and corresponding serum free Arg, His, Met, Thr, Ala, Asp, Glu, Gly, and Ser level. Our results suggest that 0.79% valine dietary supplementation can improve production performance by promoting amino acid nutrient uptake and utilization, and suggest a supplement of 0.79% valine to diet.

scholarly journals Human papillomavirus16 E6 but not E7 upregulates GLUT1 expression in lung cancer cells by upregulating thioredoxin expression

2021 ◽  
Vol 20 ◽  
pp. 153303382110671
Author(s):  
Zi-Yu Gao ◽  
Na-Jin Gu ◽  
Ming-Zhe Wu ◽  
Shi-Yu Wang ◽  
Hong-Tao Xu ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Mrna Expression ◽  
Cancer Cells ◽  
Cell Lines ◽  
Lung Cancer Cells ◽  
Expression Levels ◽  
Glut1 Protein ◽  
Glut1 Expression ◽  
E6 And E7 ◽  
Mrna Expression Levels

Background and objective: E6 and E7 proteins in human papillomavirus (HPV) 16 are major oncogenes in several types of tumors, including lung cancer. Previous studies have demonstrated that both E6 and E7 oncoproteins can upregulate GLUT1 protein and mRNA expression levels in lung cancer cells. Thus, the present study aimed to investigate the main differences in the molecular mechanisms of GLUT1 expression regulated by E6 and E7. Methods: The double directional genetic manipulation and immunofluorescence were performed to explore the molecular mechanism of E6 or E7 upregulating the expression of GLUT1 in H1299 and A549 cell lines. Results: The overexpression of E6 in well-established lung cancer cell lines upregulated thioredoxin (Trx) protein expression. Notably, plasmid transfection or small interfering RNA transfection with E7 had no regulatory effect on Trx expression. As an important disulfide reductase of the intracellular antioxidant system, Trx plays important role in maintaining oxidative stress balance and protecting cells from oxidative damage. The overexpression of Trx increased the activation of NF-κB by upregulating p65 expression and promoting p65 nuclear translocation, and further upregulated GLUT1 protein and mRNA expression levels. The results of the present study demonstrated that E6, but not E7, upregulated GLUT1 expression in lung cancer cells by activating NF-κB due to the participation of Trx. Conclusion: These results suggest that Trx plays an important role in the pathogenesis of HPV-associated lung cancer, and propose a novel therapeutic target for HPV-associated lung cancer.

scholarly journals BRCA1 mRNA expression levels as an indicator of chemoresistance in lung cancer

2004 ◽  
Vol 13 (20) ◽  
pp. 2443-2449 ◽  
Author(s):  
Miquel Taron ◽  
Rafael Rosell ◽  
Enriqueta Felip ◽  
Pedro Mendez ◽  
John Souglakos ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Mrna Expression ◽  
Expression Levels ◽  
Brca1 Mrna ◽  
Mrna Expression Levels
Sign in / Sign up

Export Citation Format

Share Document