scholarly journals BEHAVIORAL MUTANTS OF DROSOPHILA MELANOGASTER. I. ISOLATION AND MAPPING OF MUTATIONS WHICH DECREASE FLIGHT ABILITY

Genetics ◽  
1977 ◽  
Vol 87 (1) ◽  
pp. 95-104
Author(s):  
Theodore Homyk ◽  
David E Sheppard
Keyword(s):  
Drosophila Melanogaster ◽  
Genetic Mapping ◽  
X Chromosome ◽  
Flight Test ◽  
Flight Behavior ◽  
Temperature Sensitive ◽  
Flight Ability ◽  
Initial Characterization ◽  
F1 Progeny

ABSTRACT A flight test box was developed and used in the isolation and initial characterization of Drosophila melanogaster mutants defective in flight behavior. Forty-eight mutants were isolated from F1 progeny of ethyl methanesulfonate-treated males. Genetic mapping and complementation tests show that the mutations reside at thirty-four different sites on the X chromosome. Different mutants show different degrees of flight ability compared to controls. Forty-six mutations are recessive, while two appear to be semi-dominant with respect to flight behavior. In addition to flight defects, five mutants have visible defects, five behave as temperature-sensitive lethals and three exhibit abnormal electro-retinograms. Alleles of each of the previously known behavioral mutations, Hyperkinetic, ether à go-go and Shaker were found. Preliminary studies also suggest that the flight behavioral phenotype of mutations at seven sites is affected by the temperature at which the flies develop.

Microcloning and characterization of the early ecdysone puff region 2B of the X chromosome of Drosophila melanogaster

1986 ◽  
Vol 16 (1) ◽  
pp. 249-254 ◽  
Author(s):  
Joan Galcerán ◽  
Conrado Giménez ◽  
Jan Erik Edström ◽  
Marta Izquierdo
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome

scholarly journals Proteomic characterization of a temperature-sensitive conditional lethal in Drosophila melanogaster

Heredity ◽  
2009 ◽  
Vol 104 (2) ◽  
pp. 125-134 ◽  
Author(s):  
K S Pedersen ◽  
M C Codrea ◽  
C J Vermeulen ◽  
V Loeschcke ◽  
E Bendixen
Keyword(s):  
Drosophila Melanogaster ◽  
Temperature Sensitive

THE ANATOMY AND FUNCTION OF A SEGMENT OF THE X CHROMOSOME OF DROSOPHILA MELANOGASTER

Genetics ◽  
1972 ◽  
Vol 71 (1) ◽  
pp. 139-156
Author(s):  
B H Judd ◽  
M W Shen ◽  
T C Kaufman
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Functional Group ◽  
Deletion Mapping ◽  
Mutant Type ◽  
Functional Units ◽  
Average Size ◽  
And Function

ABSTRACT An average size chromomere of the polytene X chromosome of Drosophila melanogaster contains enough DNA in each haploid equivalent strand to code for 30 genes, each 1,000 nucleotides long. We have attempted to learn about the organization of chromosomes by asking how many functional units can be localized within a chromomere. This was done by 1) recovery of mutants representative of every cistron in the 3A2-3C2 region; 2) the characterization of the function of each mutant type and grouping by complementation tests; 3) the determination of the genetic and cytological position of each cistron by recombination and deletion mapping. The data clearly show one functional group per chromomere. It is postulated that a chromomere is one cistron within which much of the DNA is regulatory in function.

scholarly journals Temperature sensitivity of negative segregation distortion in Drosophila melanogaster.

Genetics ◽  
1993 ◽  
Vol 135 (3) ◽  
pp. 831-841 ◽  
Author(s):  
Y Hiraizumi
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome ◽  
Segregation Distortion ◽  
Current Model ◽  
Sensitive Period ◽  
Temperature Sensitive ◽  
Absolute Increase ◽  
Negative Segregation ◽  
Absolute Reduction ◽  
Radical Revision

Abstract Previous work has shown that the direction of segregation distortion in the SD (Segregation Distorter) system in Drosophila melanogaster can sometimes be reversed, but this was found only with rather weak distorters and the effect was not large. The present study reports large negative segregation distortion in a strong distorter, SD-72 chromosome. In the presence of a specific X chromosome, supp-X(SD), the proportion, k, of SD-72 chromosomes recovered from the SD-72/cn bw males ranges from 0.99 at 20 degrees to 0.11 at 28.5 degrees, whereas with a standard-X chromosome, k ranges from 0.99 to 0.95 for the same temperature range. The temperature-sensitive period is during spermiogenesis. Using a mating system in which the sperm supply is nearly exhausted, it was shown that the negative distortion at high temperatures is due to an absolute reduction in the number of SD-72 chromosomes and an absolute increase in the number of cn bw chromosomes recovered. After adjusting for non-SD-related temperature effects, the amount of decrease in the number of SD-72 progeny is nearly the same as the amount of increase in the number of cn bw progeny, suggesting that the dysfunction switches from a spermatid carrying one homolog to one carrying the other. Negative distortion requires a radical revision of current hypotheses for the mechanism of segregation distortion and a possible modification of the current model is suggested, based on differential recovery of dysfunction in the two homologs during spermiogenesis.

MICROCLONING AND CHARACTERIZATION OF THE EARLY ECDYSONE PUFF REGION 2B OF THE X CHROMOSOME OF DROSOPHILA MELANOGASTER

Ecdysone ◽  
1986 ◽  
pp. 249-254
Author(s):  
JOAN GALCERÁN ◽  
CONRADO GIMÉNEZ ◽  
JAN ERIK EDSTRÖM ◽  
MARTA IZQUIERDO
Keyword(s):  
Drosophila Melanogaster ◽  
X Chromosome

scholarly journals Isolation and initial characterization of a temperature-sensitive mutant of mouse FM3A cells defective in cytokinesis.

1985 ◽  
Vol 10 (1) ◽  
pp. 79-88 ◽  
Author(s):  
Hidetaro Yasumitsu ◽  
Fumio Hanaoka ◽  
Hideyo Yasuda ◽  
Yasufumi Murakami ◽  
Takemi Enomoto ◽  
...  
Keyword(s):  
Temperature Sensitive ◽  
Sensitive Mutant ◽  
Temperature Sensitive Mutant ◽  
Initial Characterization ◽  
Fm3a Cells
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