scholarly journals REC-MEDIATED RECOMBINATIONAL HOT SPOT ACTIVITY IN BACTERIOPHAGE LAMBDA II. A MUTATION WHICH CAUSES HOT SPOT ACTIVITY

Genetics ◽  
1974 ◽  
Vol 77 (3) ◽  
pp. 425-433
Author(s):  
Stephen T Lam ◽  
Mary M Stahl ◽  
Kenneth D McMilin ◽  
Franklin W Stahl
Keyword(s):  
Dna Synthesis ◽  
Hot Spot ◽  
Bacteriophage Lambda ◽  
Spot Activity ◽  
The Right ◽  
A Site

ABSTRACT Crosses have been performed which identify phage mutants (chi) which cause recombinational hot spot activity in λ. The hot spot activity is found in crosses of red- gam- chi- strains in rec+ hosts; in the crosses reported here, both the chi- mutations and the hot spot are located near the right end of the chromosome. The hot spot occurs in standard crosses as well as under conditions which block DNA synthesis, and is dependent on a functional host recB gene.—The chi mutation is shown to be dominant, but the tests do not show whether chi is a gene or a site.

scholarly journals REC-MEDIATED RECOMBINATIONAL HOT SPOT ACTIVITY IN BACTERIOPHAGE LAMBDA. I. HOT SPOT ACTIVITY ASSOCIATED WITH SPI-DELETIONS AND bio SUBSTITUTIONS

Genetics ◽  
1974 ◽  
Vol 77 (3) ◽  
pp. 409-423
Author(s):  
Kenneth D McMilin ◽  
Mary M Stahl ◽  
Franklin W Stahl
Keyword(s):  
Dna Synthesis ◽  
Genetic Marker ◽  
Density Gradient ◽  
Hot Spot ◽  
The Other ◽  
Bacteriophage Λ ◽  
Chromosomal Distribution ◽  
Recombinant Phage ◽  
Spot Activity ◽  
The Right

ABSTRACT In order to survey the distribution along the bacteriophage λ chromosome of Rec-mediated recombination events, crosses are performed using conditions which block essentially all DNA synthesis. One parent is density-labeled and carries a genetic marker in the left terminal λ gene (A), while the other parent is unlabeled and carries a genetic marker in the right terminal λ gene (R). Both parents are deleted for the λ recombination genes int and red, together with other recombination-associated genes, by virtue of either (1) a pure deletion or (2) a bio insertion-deletion. The distribution in a cesium density gradient of the resulting A+R+ recombinant phage reflects the chromosomal distribution of the recombination events which gave rise to those phage.

scholarly journals THE DISTRIBUTION OF CROSSOVERS ALONG UNREPLICATED LAMBDA BACTERIOPHAGE CHROMOSOMES

Genetics ◽  
1974 ◽  
Vol 77 (3) ◽  
pp. 395-408
Author(s):  
Franklin W Stahl ◽  
Kenneth D McMilin ◽  
Mary M Stahl ◽  
Jean M Crasemann ◽  
Stephen Lam
Keyword(s):  
Dna Synthesis ◽  
Bacteriophage Lambda ◽  
Severe Depression ◽  
Crossing Over ◽  
Density Distributions ◽  
Bacterial System ◽  
Lambda Bacteriophage ◽  
Reca Mutation ◽  
The Right

ABSTRACT The distribution of crossovers along unreplicated chromosomes of bacteriophage lambda has been examined by determining the density distributions and genotypes of particles in the progenies of crosses of density-labeled by ordinary parents in the presence of genetic blocks to replication. The Red and Rec systems combined produce crossovers primarily near the ends (especially the right end) of the chromosome. Removal of the generalized lambda recombination functions by red and gam mutations results in loss of these terminal crossovers; coupled with this loss is a disappearance of the differential dependence of recombination frequencies in terminal and central intervals on DNA synthesis. Removal of the bacterial system by a recA mutation results in severe depression of crossing over among unreplicated phage, with the few recombinants produced by the lambda system occurring near the right end.

Rec-mediated recombinational hot spot activity in bacteriophage lambda

1975 ◽  
Vol 94 (2) ◽  
pp. 203-212 ◽  
Author(s):  
Franklin W. Stahl ◽  
Jean M. Crasemann ◽  
Mary M. Stahl
Keyword(s):  
Hot Spot ◽  
Bacteriophage Lambda ◽  
Spot Activity

Expression of the Rz gene and the overlapping Rz1 reading frame present at the right end of the bacteriophage lambda genome

Gene ◽  
1993 ◽  
Vol 129 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Barbara Hanych ◽  
Sabina Kędzierska ◽  
Brigitte Walderich ◽  
Bogdan Uznański ◽  
Alina Taylor
Keyword(s):  
Bacteriophage Lambda ◽  
Reading Frame ◽  
The Right

scholarly journals Neuroendocrine Neoplasm in Cecal Appendix: A Case Report

2019 ◽  
Vol 4 (7) ◽  
pp. 118-126
Author(s):  
Pedro Walisson Gomes Feitosa ◽  
Andrezza Lobo Rodrigues ◽  
Esther Barbosa Gonçalves Felix ◽  
Maria Stella Batista de Freitas Neta ◽  
Sávio Samuel Feitosa Machado
Keyword(s):  
Case Report ◽  
Surgical Approach ◽  
Iliac Fossa ◽  
Neuroendocrine Neoplasm ◽  
Neuroendocrine Neoplasms ◽  
Histopathological Analysis ◽  
High Mitotic Activity ◽  
Precise Diagnosis ◽  
The Right ◽  
A Site

Although neuroendocrine neoplasms are relatively uncommon entities, the appendix is a site with relatively frequent emergence of this pathology and its precise diagnosis and clinical management have been a challenge for practitioners. This paper aims to present and discuss a case report of neuroendocrine neoplasia in the appendix of a patient with acute abdomen who underwent appendectomy. A 46-year-old man referred to the Emergency Department complaining of abdominal pain in the right iliac fossa and signs of peritoneal irritation. After diagnosis of acute appendicitis, the patient underwent appendectomy. In a macroscopic analysis, an area of 1.9 cm long, hardened, irregular and yellowish shapes was noted in the distal third of the appendix. Histopathological analysis showed neoplasia consisting of invasive islands of monotonous rounded epithelioid cells, large areas of necrosis, high mitotic activity, neural and angiolymphatic invasion and extension to adipose tissue compatible with invasion of the mesoappendix. Circumferential resection was compromised, suggesting the persistence of neoplasia in the patient even after the surgical approach, which would probably recommend the need for surgical approach. Therefore, the importance of sending collected materials for anatomopathological analysis is emphasized, since it helps in the clinical evaluation, in the etiological diagnosis, guides the medical conduct in the evolution of the case, as well as assisting in family mourning in cases of mortality.Keywords: Neuroendocrine tumor, Appendix, Histopathological analysis. 

GMO Reform—The Internal Market as a Site of Diversity

2019 ◽  
pp. 180-224
Author(s):  
Maria Weimer
Keyword(s):  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Regulatory Reform ◽  
Internal Market ◽  
Free Movement ◽  
Policy Changes ◽  
Risk Regulation ◽  
The Right ◽  
A Site ◽  
Gmo Cultivation

This chapter examines the legal and policy changes brought about by the 2015 reform of the regulatory framework for genetically modified organisms (GMOs). It considers the extent to which Directive 2015/412, introduced to make the 2015 regulatory reform possible and to allow for national opt-outs from GMO cultivation, helps overcome the legitimacy problems of EU risk regulation. The chapter first analyses the new EU approach to GMO cultivation via Directive 2015/412 before discussing the scope of EU harmonization in the field of GMO regulation after the adoption of this Directive. It then explains the constitutional limits of Article 114 TFEU in granting Member States the right to restrict GMO cultivation and concludes by assessing the implications of the 2015 reform for free movement of GMO products as well as highlighting the reform’s shortcomings.

Baroreflex modulation of heart rate and initiation of atrial activation in dogs

1988 ◽  
Vol 255 (3) ◽  
pp. H503-H513 ◽  
Author(s):  
R. B. Schuessler ◽  
T. E. Canavan ◽  
J. P. Boineau ◽  
J. L. Cox
Keyword(s):  
Blood Pressure ◽  
Heart Rate ◽  
Right Atrial ◽  
Autonomic Blockade ◽  
Left And Right ◽  
Atrial Activation ◽  
The Right ◽  
A Site ◽  
Induced Changes ◽  
Cardiac Nerves

In open-chest dogs, blood pressure was regulated by titrating doses of phenylephrine and nitroprusside to determine its effect on heart rate and pacemaker location. Changes in blood pressure correlated with changes in heart rate (r = 0.86). Activation time mapping demonstrated multicentric atrial activation, with a site of origin-rate relationship. The fastest pacemakers were located in the most cranial regions and slowest in the most caudal areas. In this chloralose-morphine anesthetized model, autonomic blockade with atropine and propranolol suggests that acute baroreflex-induced changes in heart rate were mediated exclusively by either increased sympathetic or parasympathetic tone and were not associated with inhibition of the opposite system. Division of right and left thoracic cardiac nerves indicated the left sympathetics participated in the baroreflex in 50% of the animals and the left parasympathetics in 90% of the animals. Both the right sympathetics and parasympathetics were active in the baroreflex in all animals. The data demonstrate that physiological heart rate response is regulated through an extensive system of right atrial pacemakers modulated by both left and right efferent cardiac nerves.

scholarly journals Rec-mediated recombinational activity of two adjacent Chi elements in bacteriophage lambda

1985 ◽  
Vol 45 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Ezra Yagil ◽  
Inna Shtromas
Keyword(s):  
Bacteriophage Lambda ◽  
The Other ◽  
The Right

SummaryChi is a sequence of eight nucleotide pairs which stimulate recBC-mediated recombination (Smith, 1983a, b). The effect of two linked Chis on recBC-mediated recombination was tested in bacteriophage lambda. It was noticed that the Chi element located on the right side of the phage chromosome is epistatic on the other Chi. These findings support a model proposed by Stahl et al. (1983) which suggests that the recombination machinery moves unidirectionaly in the phage chromosome from right to left. The results also suggest that in the presence of more than one Chi only the rightmost one stimulates recombination.

scholarly journals Analysis of the outer divertor hot spot activity in the protection video camera recordings at JET

2019 ◽  
Vol 139 ◽  
pp. 115-123
Author(s):  
A. Drenik ◽  
S. Brezinsek ◽  
P. Carvalho ◽  
V. Huber ◽  
N. Osterman ◽  
...  
Keyword(s):  
Hot Spot ◽  
Video Camera ◽  
Spot Activity
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