scholarly journals CHEMICAL SELECTION OF ALCOHOL DEHYDROGENASE NEGATIVE MUTANTS IN DROSOPHILA

Genetics ◽  
1972 ◽  
Vol 72 (3) ◽  
pp. 545-549
Author(s):  
William H Sofer ◽  
Mark A Hatkoff
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Selection Procedure ◽  
Short Exposure ◽  
Unsaturated Alcohol ◽  
Wild Type ◽  
Alcohol Dehydrogenase Activity ◽  
Large Populations ◽  
Chemical Selection ◽  
Selection Of

ABSTRACT We describe a selection procedure which utilizes the vapor from an unsaturated alcohol, 1-pentene-3-ol, for the detection and isolation of mutant flies with little or no alcohol dehydrogenase activity. ADH-negative flies are unaffected by exposure to the unsaturated alcohol, but ADH positives (wild-types) die after short exposure. The technique can be used to select rare ADH-negative individuals from large populations of wild-type flies.

scholarly journals CHEMICAL SELECTION OF MUTANTS THAT AFFECT ALCOHOL DEHYDROGENASE IN DROSOPHILA II. USE OF 1—PENTYNE—3—Ol

Genetics ◽  
1975 ◽  
Vol 79 (1) ◽  
pp. 73-83
Author(s):  
Janis O'Donnell ◽  
Larry Gerace ◽  
Flora Leister ◽  
William Sofer
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Culture Medium ◽  
Low Concentrations ◽  
Alcohol Dehydrogenase Activity ◽  
Eggs And Larvae ◽  
Chemical Selection ◽  
Selection Of

ABSTRACT We describe a procedure for the selection of alcohol dehydrogenase negative mutants in Drosophila. The method consists of exposing eggs and larvae to low concentrations of 1-pentyne-3-ol dissolved in the culture medium. Only those flies with greatly reduced levels of alcohol dehydrogenase activity survive. In addition, genotypically negative flies die if their mothers are alcohol dehydrogenase positive. Using this procedure and formaldehyde to generate mutants, we were able to detect seven alcohol dehydrogenase negative mutants out of 350,000 individuals subjected to selection. At least five of the mutants contain small deletions that include the alcohol dehydrogenase locus.

The effect of respiratory deficiency on the alcohol dehydrogenase activity of baker's yeast

1972 ◽  
Vol 18 (1) ◽  
pp. 23-28 ◽  
Author(s):  
H. M. C. Heick
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Isocitrate Lyase ◽  
Heat Stability ◽  
Relative Activity ◽  
Wild Type ◽  
Respiratory Deficiency ◽  
Alcohol Dehydrogenase Activity ◽  
Ethanol Medium ◽  
Respiratory Deficient

The effects of respiratory deficiency on the level of NAD-dependent alcohol dehydrogenase activity were studied. Respiratory-deficient mutants produced by acriflavine, and wild-type cells grown on glucose in the presence of chloramphenicol had elevated alcohol dehydrogenase activities compared with the wild-type cells grown on glucose. The increased activity was a result of an increase in ADH-I. The respiratory-deficient mutants appeared unable to produce ADH-II or isocitrate lyase even after incubation of the cells in ethanol medium. However, this transfer to ethanol medium increased the level of ADH-I activity. The presence of chloramphenicol in the ethanol medium further increased the alcohol dehydrogenase activity of these mutants. The effect of chloramphenicol in the wild-type cultures depended on the time of its addition to the culture. If chloramphenicol was present during both the period of growth on glucose and in the ethanol medium, ADH-II activity did not appear. If the cultures were grown on glucose in the absence of chloramphenicol, allowing respiration to develop, the subsequent presence of chloramphenicol in fresh ethanol medium did not prevent the appearance of either ADH-II or isocitrate lyase activity. The respiratory-deficient mutants also contained alcohol dehydrogenase activity which resembles ADH-III in respect to electrophoretic mobility, heat stability, and relative activity with various alcohols as substrates.

ALCOHOL DEHYDROGENASE ACTIVITY IN ISOLATED VACUOLES OF RED BEET ROOT CELLS

Tsitologiya ◽  
2018 ◽  
Vol 60 (6) ◽  
pp. 469-475
Author(s):  
O. D. Nimaeva ◽  
◽  
E. V. Pradedova ◽  
A. B. Karpova ◽  
R. K. Salyaev ◽  
...  
Keyword(s):  
Alcohol Dehydrogenase ◽  
Dehydrogenase Activity ◽  
Root Cells ◽  
Beet Root ◽  
Red Beet ◽  
Alcohol Dehydrogenase Activity

Elevation of Alcohol Dehydrogenase Activity in the Subclinically Scorbutic Guinea-Pig

1972 ◽  
Vol 42 (6) ◽  
pp. 781-784 ◽  
Author(s):  
Maureen O'keane ◽  
M. R. Moore ◽  
A. Goldberg
Keyword(s):  
Alcohol Dehydrogenase ◽  
Guinea Pig ◽  
Dehydrogenase Activity ◽  
Guinea Pigs ◽  
Liver Protein ◽  
Alcohol Dehydrogenase Activity ◽  
Nadh Ratio

1. Because it has been shown that a majority of alcoholics are subclinically scorbutic, the metabolism of ethanol was studied in subclinically-scorbutic guinea-pigs. 2. Hepatic alcohol dehydrogenase activity was raised maximally by ethanol within 2 days. 3. In twenty-three subclinically-scorbutic guinea-pigs fed ethanol for 2 weeks, the alcohol dehydrogenase activity (±SD) was 11·5 ± 1·2 units/g of liver protein compared with 8·6 ± 0·6 units/g of liver protein in twenty-three healthy animals fed ethanol. 4. The NAD+/NADH ratio in subclinically-scorbutic guinea-pigs and healthy guinea-pigs fed ethanol, shows that there is more NAD+ available for oxidation of alcohol in subclinically-scorbutic guinea-pigs. These results may explain the increased tolerance of alcoholics to alcohol.

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