Roles of ABF1, NPL3, and YCL54 in silencing in Saccharomyces cerevisiae.

Genetics ◽  
1995 ◽  
Vol 141 (3) ◽  
pp. 889-902 ◽  
Author(s):  
S Loo ◽  
P Laurenson ◽  
M Foss ◽  
A Dillin ◽  
J Rine
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Binding ◽  
Binding Sites ◽  
Genetic Screen ◽  
Essential Genes ◽  
Temperature Sensitive ◽  
Protein Binding Sites ◽  
Temperature Sensitive Mutation

Abstract A sensitized genetic screen was carried out to identify essential genes involved in silencing in Saccharomyces cerevisiae. This screen identified temperature-sensitive alleles of ORC2 and ORC5, as described elsewhere, and ABF1, NPL3, and YCL54, as described here. Alleles of ABF1 that caused silencing defects provided the genetic proof of Abflp's role in silencing. The roles of Npl3p and Ycl54p are less clear. These proteins did not act exclusively through any one of the three protein binding sites of the HMR-E silencer. Unlike the orc2, orc5, and abf1 mutations that were isolated in the same (or a similar) screen for silencing mutants, neither temperature-sensitive mutation in NPL3 or YCL54 caused overt replication defects.

scholarly journals Pervasive and dynamic protein binding sites of the mRNA transcriptome in Saccharomyces cerevisiae

2013 ◽  
Vol 14 (2) ◽  
pp. R13 ◽  
Author(s):  
Mallory A Freeberg ◽  
Ting Han ◽  
James J Moresco ◽  
Andy Kong ◽  
Yu-Cheng Yang ◽  
...  
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Protein Binding ◽  
Binding Sites ◽  
Protein Binding Sites ◽  
Mrna Transcriptome

EVALUATION OF TISSUE STEROID BINDING IN VITRO

1971 ◽  
Vol 68 (1_Suppl) ◽  
pp. S223-S246 ◽  
Author(s):  
C. R. Wira ◽  
H. Rochefort ◽  
E. E. Baulieu
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Experimental System ◽  
Specific Protein ◽  
Coupling Mechanism ◽  
Target Tissue ◽  
Protein Binding Sites ◽  
Definition Of ◽  
Hormone Specificity

ABSTRACT The definition of a RECEPTOR* in terms of a receptive site, an executive site and a coupling mechanism, is followed by a general consideration of four binding criteria, which include hormone specificity, tissue specificity, high affinity and saturation, essential for distinguishing between specific and nonspecific binding. Experimental approaches are proposed for choosing an experimental system (either organized or soluble) and detecting the presence of protein binding sites. Techniques are then presented for evaluating the specific protein binding sites (receptors) in terms of the four criteria. This is followed by a brief consideration of how receptors may be located in cells and characterized when extracted. Finally various examples of oestrogen, androgen, progestagen, glucocorticoid and mineralocorticoid binding to their respective target tissues are presented, to illustrate how researchers have identified specific corticoid and mineralocorticoid binding in their respective target tissue receptors.

scholarly journals Transcription signals and protein binding sites for sericin gene transcription in vitro

1989 ◽  
Vol 264 (31) ◽  
pp. 18707-18713 ◽  
Author(s):  
K Matsuno ◽  
C C Hui ◽  
S Takiya ◽  
T Suzuki ◽  
K Ueno ◽  
...  
Keyword(s):  
Protein Binding ◽  
Gene Transcription ◽  
Binding Sites ◽  
Protein Binding Sites ◽  
Transcription In Vitro ◽  
Transcription Signals

scholarly journals Structure of the canine pancreatic colipase gene includes two protein-binding sites in the promoter region

1993 ◽  
Vol 268 (15) ◽  
pp. 11312-11320
Author(s):  
S. Fukuoka ◽  
D.E. Zhang ◽  
Y. Taniguchi ◽  
G.A. Scheele
Keyword(s):  
Protein Binding ◽  
Binding Sites ◽  
Promoter Region ◽  
Protein Binding Sites
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