A genetic map of the mouse suitable for typing intraspecific crosses.

Genetics ◽  
1992 ◽  
Vol 131 (2) ◽  
pp. 423-447 ◽  
Author(s):  
W Dietrich ◽  
H Katz ◽  
S E Lincoln ◽  
H S Shin ◽  
J Friedman ◽  
...  
Keyword(s):  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Mouse Genome ◽  
Sequence Length ◽  
Chain Reaction ◽  
Average Spacing ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
High Degree ◽  
Simple Sequence

Abstract We report the construction of a genetic linkage map of the mouse, consisting entirely of genetic markers that can be rapidly typed by polymerase chain reaction and that show a high degree of polymorphism among inbred laboratory strains. Specifically, the map contains 317 simple sequence length polymorphisms at an average spacing of 4.3 cM and is detectably linked to approximately 99% of the mouse genome. In typical crosses between inbred laboratory strains, about 50% of the markers are polymorphic, making it straightforward to follow inheritance in almost any cross.

scholarly journals PCR-based microsatellite analysis for differentiation and genetic monitoring of nine inbred SENCAR mouse strains

2001 ◽  
Vol 35 (2) ◽  
pp. 157-162 ◽  
Author(s):  
F. Benavides ◽  
E. Glasscock ◽  
L. G. Coghlan ◽  
M. C. Stern ◽  
D. A. Weiss ◽  
...  
Keyword(s):  
Genetic Basis ◽  
Sequence Length ◽  
Mouse Strains ◽  
Mechanistic Studies ◽  
Chain Reaction ◽  
Dna Microsatellites ◽  
Multi Stage ◽  
Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

Sixteen DNA microsatellites or simple sequence length polymorphisms (SSLPs), generated by polymerase chain reaction (PCR) were selected for use in the genetic quality control of the nine inbred SENCAR strains currently available. The SENCAR strains constitute a powerful tool for mechanistic studies of multi-stage skin carcinogenesis, as well as for studies to understand the underlying genetic basis of resistance to tumour promotion and progression. SSLP analysis is a fast and economical way for detecting genetic contamination (unexpected outcrosses) among these closely-related albino strains, where standard immunological and biochemical markers have been shown to be insufficient.

Genetic linkage map of medaka with polymerase chain reaction length polymorphisms

Gene ◽  
2005 ◽  
Vol 363 ◽  
pp. 24-31 ◽  
Author(s):  
Tetsuaki Kimura ◽  
Keiko Yoshida ◽  
Atsuko Shimada ◽  
Tomoko Jindo ◽  
Mitsuru Sakaizumi ◽  
...  
Keyword(s):  
Polymerase Chain Reaction ◽  
Linkage Map ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Chain Reaction ◽  
Length Polymorphisms ◽  
Polymerase Chain

A reference cross DNA panel for zebrafish (Danio rerio) anchored with simple sequence length polymorphisms

Development ◽  
1996 ◽  
Vol 123 (1) ◽  
pp. 451-460 ◽  
Author(s):  
E.W. Knapik ◽  
A. Goodman ◽  
O.S. Atkinson ◽  
C.T. Roberts ◽  
M. Shiozawa ◽  
...  
Keyword(s):  
Linkage Map ◽  
Genetic Markers ◽  
Genetic Linkage ◽  
Positional Cloning ◽  
Genetic Linkage Map ◽  
Physical Map ◽  
Sequence Length ◽  
Mapping Tool ◽  
Length Polymorphisms ◽  
Simple Sequence

The ultimate informativeness of the zebrafish mutations described in this issue will rest in part on the ability to clone these genes. However, the genetic infrastructure required for the positional cloning in zebrafish is still in its infancy. Here we report a reference cross panel of DNA, consisting of 520 F2 progeny (1040 meioses) that has been anchored to a zebrafish genetic linkage map by 102 simple sequence length polymorphisms. This reference cross DNA provides: (1) a panel of DNA from the cross that was used to construct the genetic linkage map, upon which polymorphic gene(s) and genetic markers can be mapped; (2) a fine order mapping tool, with a maximum resolution of 0.1 cM; and (3) a foundation for the development of a physical map (an ordered array of clones each containing a known portion of the genome). This reference cross DNA will serve as a resource enabling investigators to relate genes or genetic markers directly to a single genetic linkage map and avoid the problem of integrating different maps with different genetic markers, as must be currently done when using randomly amplified polymorphic DNA markers, or as has occurred with human genetic linkage maps.

scholarly journals Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species Marka molekuler dan penerapannya untuk studi sidik jari DNA dan keragaman genetik pada spesies Coffea

2016 ◽  
Vol 82 (1) ◽  
Author(s):  
. PRIYONO ◽  
Riza Arief PUTRANTO
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Dna Fingerprinting ◽  
Fragment Length ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

AbstrakStrategi klasik yang meliputi perbandingan anatomi, fisiologi dan sitogenetika telah banyak diterapkan untuk mengidentifikasi karakter tertentu serta untuk menentukan keragaman dan hubungan antar dan intra spesies. Namun, saat ini penanda molekuler telah melengkapi strategi sebelumnya dengan sangat cepat. Berbagai jenis penanda molekuler digunakan untuk menilai tingkat polimorfisme DNA. Penanda molekuler ini diklasifikasikan sebagai penanda berbasis hibridisasi dan berbasis Polymerase Chain Reaction (PCR). Dalam beberapa tahun terakhir, sistem penanda DNA yang berbeda seperti Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) yang juga disebut Mikrosatelit, Single Nucleotide Polymorphims (SNPs) dan lain-lain telah dikembangkan dan diterapkan pada berbagai spesies tanaman. Penanda molekuler ini dapat digunakan untuk sidik jari DNA dan studi keragaman genetik. Sidik jari berdasarkan DNA telah banyak digunakan dalam ilmu forensik, juga memiliki berbagai aplikasi dalam pemuliaan tanaman. Tulisan ini memberikan overview tentang berbagai penanda molekuler dan aplikasinya untuk sidik jari dan kajian keragaman genetik tanaman berdasarkan DNA pada berbagai spesies tanaman, dan secara khusus pada Coffea sp.AbstractConventional strategies including comparative anatomy, physiology and cytogenetics were applied to identify the certain character as well as to determine inter- and intra-species diversity and relationships. However, more recently molecular markers have very rapidly complemented the previous strategies. Various types of molecular markers are used to assess DNA polymorphism. They are classified as hybridization-based markers and polymerase chain reaction (PCR) based markers. In recent years, different DNA marker systems such as Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which also called as microsatellites, Single Nucleotide  Polymorphims  (SNPs)  and  others  have been developed and applied to a range of plant species. These molecular markers can be used for DNA fingerprinting and genetic diversity study. DNA fingerprinting has been widely used in forensic science, but is has also a variety of application in plant breeding. This paper provides an overview about various molecular markers and their application for DNA plant fingerprinting and genetic diversity, especially in Coffea sp.

scholarly journals Molecular markers and their application for DNA fingerprinting and genetic diversity studies in Coffea species Marka molekuler dan penerapannya untuk studi sidik jari DNA dan keragaman genetik pada spesies Coffea

2016 ◽  
Vol 82 (1) ◽  
Author(s):  
. PRIYONO ◽  
Riza Arief PUTRANTO
Keyword(s):  
Genetic Diversity ◽  
Molecular Markers ◽  
Dna Fingerprinting ◽  
Fragment Length ◽  
Chain Reaction ◽  
Single Nucleotide ◽  
Length Polymorphisms ◽  
Amplified Fragment Length Polymorphisms ◽  
Polymerase Chain ◽  
Simple Sequence

AbstrakStrategi klasik yang meliputi perbandingan anatomi, fisiologi dan sitogenetika telah banyak diterapkan untuk mengidentifikasi karakter tertentu serta untuk menentukan keragaman dan hubungan antar dan intra spesies. Namun, saat ini penanda molekuler telah melengkapi strategi sebelumnya dengan sangat cepat. Berbagai jenis penanda molekuler digunakan untuk menilai tingkat polimorfisme DNA. Penanda molekuler ini diklasifikasikan sebagai penanda berbasis hibridisasi dan berbasis Polymerase Chain Reaction (PCR). Dalam beberapa tahun terakhir, sistem penanda DNA yang berbeda seperti Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) yang juga disebut Mikrosatelit, Single Nucleotide Polymorphims (SNPs) dan lain-lain telah dikembangkan dan diterapkan pada berbagai spesies tanaman. Penanda molekuler ini dapat digunakan untuk sidik jari DNA dan studi keragaman genetik. Sidik jari berdasarkan DNA telah banyak digunakan dalam ilmu forensik, juga memiliki berbagai aplikasi dalam pemuliaan tanaman. Tulisan ini memberikan overview tentang berbagai penanda molekuler dan aplikasinya untuk sidik jari dan kajian keragaman genetik tanaman berdasarkan DNA pada berbagai spesies tanaman, dan secara khusus pada Coffea sp.AbstractConventional strategies including comparative anatomy, physiology and cytogenetics were applied to identify the certain character as well as to determine inter- and intra-species diversity and relationships. However, more recently molecular markers have very rapidly complemented the previous strategies. Various types of molecular markers are used to assess DNA polymorphism. They are classified as hybridization-based markers and polymerase chain reaction (PCR) based markers. In recent years, different DNA marker systems such as Restriction Fragment Length Polymorphisms (RFLPs), Random Amplied Polymorphic DNAs (RAPDs), Amplified Fragment Length Polymorphisms (AFLPs), Simple Sequence Repeats (SSRs) which also called as microsatellites, Single Nucleotide  Polymorphims  (SNPs)  and  others  have been developed and applied to a range of plant species. These molecular markers can be used for DNA fingerprinting and genetic diversity study. DNA fingerprinting has been widely used in forensic science, but is has also a variety of application in plant breeding. This paper provides an overview about various molecular markers and their application for DNA plant fingerprinting and genetic diversity, especially in Coffea sp.

scholarly journals Genetic Diversity among Cucumis metuliferus Populations Revealed by Cucumber Microsatellites

HortScience ◽  
2010 ◽  
Vol 45 (2) ◽  
pp. 214-219 ◽  
Author(s):  
Yiqun Weng
Keyword(s):  
Genetic Diversity ◽  
Genetic Variation ◽  
Dna Sequences ◽  
Clustering Analysis ◽  
Simple Sequence Repeat ◽  
Neighbor Joining ◽  
Chain Reaction ◽  
Polymerase Chain ◽  
High Degree ◽  
Simple Sequence

Cucumis metuliferus E. Mey. ex Naud (African horned cucumber, horned melon) is endemic to Africa and is a relative of cultivated cucumber (C. sativus L.) and melon (C. melo L.). In the present study, genetic variation among the USDA C. metuliferus collection was evaluated with microsatellite [simple sequence repeat (SSR)] markers derived from C. sativus genomic DNA sequences. Of 564 cucumber SSRs tested, 51.8% were able to produce polymerase chain reaction amplicons in C. metuliferus suggesting a high degree of DNA sequence homology between the two species. Forty-two cross-species SSRs were used to assess genetic variation among 36 C. metuliferus accessions. Genetic diversity among these accessions was relatively low. Of the 42 SSRs, 12 were monomorphic, and each marker, on average, was able to detect 3.3 alleles among the 36 accessions. Neighbor-joining clustering analysis revealed a positive relationship between genetic divergence and geographic distances among these accessions. Genetic distance of C. metuliferus to melon is smaller than that of C. metuliferus to cucumber.

The SUSPECT SYMPTOMS OF INBREEDING DEPRESSION AND THE HOMOZYGOSITY LEVEL OF FOURTH GENERATION OF SP450T AND FIFTH GENERATION OF DURA DELI OIL PALM SELFING POPULATION

2018 ◽  
Vol 24 (2) ◽  
pp. 55-66
Author(s):  
Rokhana Faizah ◽  
Sri Wening ◽  
Hernawan Yuli Rahmadi ◽  
Abdul Razak Purba
Keyword(s):  
Inbreeding Depression ◽  
Oil Palm ◽  
Recurrent Selection ◽  
Fourth Generation ◽  
Depression Symptoms ◽  
Reciprocal Recurrent Selection ◽  
Chain Reaction ◽  
Fifth Generation ◽  
Polymerase Chain ◽  
Simple Sequence

Inbreeding is a common method used to reproduce candidate mother plant from selected parental lines for commercial seeds in Reciprocal Recurrent Selection (RRS) oil palm breeding program. However this practice may increased homozigosity level of selected population. This study concerned the level of homozygosity of SP540T fourth generations and Dura Deli Dolok Sinumbah fifth generations (3 crosses respectively) and their correlation with inbreeding depression symptoms. Polymerase Chain Reaction-Simple Sequence Repeat (PCR-SSR) with 16 markers developed for oil palm was used to analyze 327 samples. The result shows that the levels of homozigosity of SP540T fourth selfing generation were ranged between 0.44-0.84 or 0.61 in average. While the levels of homozygosity of Dura Deli fifth selfing generations were ranged between 0.60-0.93 or 0.78 in average. The homozygosity level in Dura Deli was 1.27% higher than SP540T populations. Correlation analysis showed that the higher the level of homozygosity, the higher of the inbreeding symptoms 2 observed (R =0.95).

Detection of polymorphic simple-sequence repeat markers that show linkage to a novel sugarcane brown rust disease resistance gene in resistant and susceptible genetic pools

2020 ◽  
pp. 1-7
Author(s):  
Jie Li ◽  
Xiao-Yan Wang ◽  
Hong-Li Shan ◽  
Rong-Yue Zhang ◽  
Chan-Mi Wang ◽  
...  
Keyword(s):  
Resistance Genes ◽  
Genetic Linkage ◽  
Genetic Linkage Map ◽  
Brown Rust ◽  
Cross Combination ◽  
Map Construction ◽  
Parental Lines ◽  
Genetic Pools ◽  
Simple Sequence ◽  
Rust Resistance Genes

Abstract Sugarcane brown rust, caused by Puccinia melanocephala, is one of the main diseases of sugarcane in China. The identification and discovery of new resistance genes have important theoretical and practical significance for preventing outbreaks of brown rust and ensuring the sustainable production of sugarcane. To screen for polymorphic simple-sequence repeat (SSR) molecular markers for localization of brown rust resistance genes, we used two populations that are suitable for genetic linkage map construction and mapping of new resistance genes to construct resistant and susceptible genetic pools. We then screened 449 pairs of primers to identify polymorphic SSR markers in the parental lines and the resistant/susceptible genetic pools. The results showed that 25 pairs of primers directed amplification of polymorphic DNA fragments between the parents of the cross combination ‘Yuetang 03-393’ × ‘ROC 24’, and 16 pairs of primers amplified polymorphic fragments between the parents of the cross combination ‘Liucheng 03-1137’ × ‘Dezhe 93-88’. Four pairs of primers (SMC236CG, SCESSR0928, SCESSR0636 and SCESSR2551) amplified polymorphic DNA fragments between the parental lines and the resistant/susceptible genetic pools in ‘Yuetang 03-393’ × ‘ROC 24’. The results of this study will establish a solid foundation for the mapping of new brown rust resistance genes, genetic linkage map construction and the development of closely-associated molecular markers in sugarcane.

Polymerase chain reaction in the diagnosis of parotid gland tuberculosis

1998 ◽  
Vol 112 (5) ◽  
pp. 494-496 ◽  
Author(s):  
Enis Alpin Güneri ◽  
Ahmet Ömer İkiz ◽  
Nese Atabey ◽  
Özlem İzci ◽  
Semih Sütay
Keyword(s):  
Polymerase Chain Reaction ◽  
Differential Diagnosis ◽  
Parotid Gland ◽  
Mycobacterial Infection ◽  
Social Characteristics ◽  
Causative Organism ◽  
Chain Reaction ◽  
First Case ◽  
Polymerase Chain ◽  
High Degree

AbstractA parotid gland mass with presenting features of malignancy is a diagnostic and therapeutic challenge. The histological nature of the lesion must be clearly determined before proceeding with facial nerve sacrificing surgery. Although rare, tuberculosis of the parotid gland must be included in the differential diagnosis of a parotid gland mass especially when the social characteristics of the patient suggests a mycobacterial infection. Primary tuberculosis of the parotid gland is generally encountered among populations with a high incidence of pulmonary disease. The difficulty in the differential diagnosis of a parotid gland malignancy may be helped by a high degree of clinical suspicion, since laboratory tests generally do not identify the specific causative organism. This article reports the first case of parotid gland tuberculosis with clinical and radiodiagnostical features simulating malignancy in which the diagnosis was confirmed by the polymerase chain reaction (PCR).

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