scholarly journals A linkage map of endogenous murine leukemia proviruses.

Genetics ◽  
1990 ◽  
Vol 124 (2) ◽  
pp. 221-236 ◽  
Author(s):  
W N Frankel ◽  
J P Stoye ◽  
B A Taylor ◽  
J M Coffin
Keyword(s):  
Linkage Map ◽  
Recombinant Inbred ◽  
Inbred Mice ◽  
Recombinant Inbred Strain ◽  
Leukemia Virus ◽  
Distribution Patterns ◽  
Inbred Strains ◽  
Marker Genes ◽  
Linkage Data ◽  
Murine Leukemia

Abstract Thirty endogenous proviruses belonging to the modified polytropic (Mpmv) class of murine leukemia virus (MLV) were identified by proviral-cellular DNA junction fragment segregation in several sets of recombinant inbred mice. Twenty-six Mpmv loci were mapped to chromosomal regions by matching proviral strain distribution patterns to those of previously assigned genes. Like other endogenous nonecotropic MLVs, Mpmv loci were present on several chromosomes in all strains examined. We pooled recombinant inbred strain linkage data from 110 MLV loci and selected marker genes in order to construct a chromosomal linkage map. Every mouse chromosome was found to harbor at least one proviral insertion, and several regions contained multiple integrations. However, the overall distribution of the 110 mapped proviruses did not deviate significantly from a random distribution. Because of their polymorphism in inbred strains of mice, and the ability to score as many as 57 proviruses per strain using only three hybridization probes, the nonecotropic MLVs mapped in common strains of mice offer a significant advantage over older methods (e.g., biochemical or individual restriction fragment polymorphisms) as genetic markers. These endogenous insertion elements should also be useful for assessing strain purity, and for studying the relatedness of common and not-so-common inbred strains.

scholarly journals Expression of a monoclonal antibody-defined, B-lineage transformation antigen specifically identifies Abelson-diseased animals. Genetically determined resistance to Abelson murine leukemia virus acts before induction of gp160(6C3).

1986 ◽  
Vol 164 (4) ◽  
pp. 1356-1361 ◽  
Author(s):  
G F Tidmarsh ◽  
M O Dailey ◽  
I L Weissman
Keyword(s):  
Recombinant Inbred ◽  
Recombinant Inbred Lines ◽  
Leukemia Virus ◽  
Neoplastic Transformation ◽  
Inbred Strains ◽  
Inbred Lines ◽  
Abelson Murine Leukemia Virus ◽  
B Lineage ◽  
Genetically Determined ◽  
Murine Leukemia

Mice genetically susceptible or genetically resistant to the leukemogenic effects of A-MuLV(Mo) were tested for their expression of the B-lineage neoplastic transformation-associated antigen, 6C3Ag. Genetically resistant inbred strains and recombinant inbred lines developed neither cells expressing high levels of 6C3Ag (6C3Aghi) in their hematolymphoid tissues nor Abelson leukemias. Genetically susceptible inbred strains and recombinant inbred lines developed high percentages of 6C3Aghi hematolymphoid cells concomitant with development of Abelson leukemias and lymphomas. Thus the genetically-determined resistance to A-MuLV(Mo) leukemogenesis appears to act at some step(s) after virus infection but before the stage of malignant progression, which is marked by 6C3Ag expression.

scholarly journals Three-locus linkage analysis using recombinant inbred strains and Bayes' theorem.

Genetics ◽  
1991 ◽  
Vol 128 (3) ◽  
pp. 631-638
Author(s):  
P E Neumann
Keyword(s):  
Linkage Analysis ◽  
Bayesian Analysis ◽  
Linkage Map ◽  
Recombinant Inbred ◽  
Inbred Strains ◽  
Bayes Theorem ◽  
Linkage Data ◽  
Marker Loci ◽  
Linkage Detection ◽  
Genomic Regions

Abstract Recombinant inbred (RI) strains are useful in linkage analysis and gene mapping. However, the generally small number of strains in an RI strain set limits the power of RI strains in linkage detection. Several methods for increasing the power of RI strains have been used, including summing data across RI strain sets and excluding linkage to genomic regions. In this paper, Bayesian analysis is applied to three-locus linkage data. This method further increases the power of RI strains to detect linkage and gives estimations of the probability of each of the three possible gene orders if the test locus is linked to the pair of marker loci. Several examples are presented, including reconsideration of the position of the proto-oncogene L-myc on the mouse linkage map.

The NXSM recombinant inbred strains of mice: genetic profile for 58 loci including the Mtv proviral loci.

Genetics ◽  
1990 ◽  
Vol 125 (2) ◽  
pp. 431-446
Author(s):  
E M Eicher ◽  
B K Lee
Keyword(s):  
X Chromosome ◽  
Mammary Tumor ◽  
Recombinant Inbred ◽  
Inbred Strains ◽  
Genetic Profile ◽  
Inbred Strains Of Mice ◽  
Strain Data ◽  
Mink Cell ◽  
Using Data ◽  
Murine Leukemia

Abstract We report the construction of 17 recombinant inbred (RI) strains of mice derived from the progenitor strains NZB/BINRe and SM/J and the typing of this RI strain set, designated NXSM, for 58 loci distributed on 16 autosomes and the X chromosome. Two backcrosses involving NZB/BINJ and SM/J were constructed to confirm chromosomal assignments and determine gene orders suggested from NXSM RI strain data. From these results we recommend that chromosomal assignments and gene orders suggested from analyses of RI strain sets be confirmed using data obtained by other means. We also typed NZB/BINJ and SM/J for mammary tumor proviral (Mtv) loci. Both strains share three previously described Mtv loci: Mtv-7, Mtv-14 and Mtv-17. In addition, NZB/BINJ contains the previously described Mtv-3 and Mtv-9 loci and two new Mtv proviral loci: Mtv-27 located on chromosome (Chr) 1 and Mtv-28 located on the X chromosome. SM/J contains the previously described loci Mtv-6 and Mtv-8. Four LTR, mink cell focus-forming murine leukemia viral loci were identified and mapped: Ltrm-1 on Chr 12, Ltrm-2 on Chr 16, Ltrm-3 on Chr 5, and Ltrm-4 on Chr 13. The Tgn locus was positioned proximal to the Ly-6 locus on Chr 15.

scholarly journals Expression of xenotropic murine leukemia viruses as cell-surface gp70 in genetic crosses between strains DBA/2 and C57BL/6.

1979 ◽  
Vol 149 (5) ◽  
pp. 1183-1196 ◽  
Author(s):  
H C Morse ◽  
T M Chused ◽  
J W Hartley ◽  
B J Mathieson ◽  
S O Sharrow ◽  
...  
Keyword(s):  
Cell Surface ◽  
Leukemia Virus ◽  
Fluorescent Antibody ◽  
Virus Production ◽  
Inbred Strains ◽  
Cell Surface Antigens ◽  
Virus Envelope ◽  
Xenotropic Murine Leukemia ◽  
Flow Microfluorometry ◽  
Murine Leukemia

Flow microfluorometry was used to assess levels of xenotropic murine leukemia virus envelope-related cell-surface antigens (XenCSA) expressed on lymphocytes of mice derived from crosses between C57BL/6 (B6) and DBA/2 (D2); 24 recombinant inbred strains (BXD RIs) and 62 backcross mice were studied. The results suggest that XenCSA expression is affected by more than one gene but that the predominant influence is exerted by a single semidominant gene apparently located on chromosome 4 at or in close proximity to the Fv-1 locus. Studies of spontaneous virus production in B6D2F1 X D2 mice suggest that this locus may also affect production by spleen cells of xenotropic MuLV registering in a fluorescent antibody assay of mink lung cells.

Identification of a common ecotropic viral integration site, Evi-1, in the DNA of AKXD murine myeloid tumors

1988 ◽  
Vol 8 (1) ◽  
pp. 301-308
Author(s):  
M L Mucenski ◽  
B A Taylor ◽  
J N Ihle ◽  
J W Hartley ◽  
H C Morse ◽  
...  
Keyword(s):  
B Cell ◽  
Recombinant Inbred ◽  
High Frequency ◽  
Integration Site ◽  
Inbred Mice ◽  
Myeloid Cell ◽  
Inbred Strains ◽  
Viral Integration ◽  
Recombinant Inbred Mice ◽  
T Cell Lymphomas

AKXD-23 recombinant inbred mice develop myeloid tumors at a high frequency, unlike other AKXD recombinant inbred strains which develop B-cell lymphomas, T-cell lymphomas, or both. AKXD-23 myeloid tumors are monoclonal, and their DNA contains somatically acquired proviruses, suggesting that they are retrovirally induced. We identified a common site of ecotropic proviral integration that is present in the DNA of all AKXD-23 myeloid tumors that were analyzed and in the DNA of all myeloid tumors that occur in AKXD strains other than AKXD-23. We designated this locus Evi-1 (ecotropic viral integration site 1). Rearrangements in the Evi-1 locus were also detected in the DNA of a number of myeloid tumors and myeloid cell lines isolated from strains other than AKXD. In contrast, few Evi-1 rearrangements were detected in the DNA of T- or B-cell tumors. Evi-1 may thus identify a new proto-oncogene locus that is involved in myeloid disease.

scholarly journals Systems Genetics and Systems Biology Analysis of Paraquat Neurotoxicity in BXD Recombinant Inbred Mice

2020 ◽  
Vol 176 (1) ◽  
pp. 137-146
Author(s):  
Carolina Torres-Rojas ◽  
Daming Zhuang ◽  
Paola Jimenez-Carrion ◽  
Isabel Silva ◽  
James P O’Callaghan ◽  
...  
Keyword(s):  
Parkinson’S Disease ◽  
Parkinson's Disease ◽  
Recombinant Inbred ◽  
Inbred Mice ◽  
Recombinant Inbred Strain ◽  
Iron Concentration ◽  
Differential Susceptibility ◽  
The United States ◽  
Ventral Midbrain ◽  
Sporadic Parkinson’S Disease

Abstract Paraquat (PQ) is an herbicide used in many countries, including the United States. It is also implicated as a risk factor for sporadic Parkinson’s disease, especially in those living in agricultural areas and drinking well water. Studies linking PQ to sporadic Parkinson’s disease are not consistent however and there appears to be interindividual differential susceptibility. One likely reason is genetically based differential susceptibility to paraquat neurotoxicity in subpopulations. To address this issue, we tested the effects of paraquat in a genetic reference population of mice (the BXD recombinant inbred strain family). In our earlier work, we showed that in genetically susceptible mice, paraquat increases iron in the ventral midbrain, the area containing the substantia nigra. Our hypothesis is that genetic variability contributes to diverse PQ-related susceptibility and iron concentration. To test this hypothesis, we treated male mice from 28 to 39 BXD strains plus the parental strains with 1 of 3 doses of paraquat, 1, 5, and 10 mg/kg 3 times on a weekly basis. At the end of the treatment period, we analyzed the ventral midbrain for concentrations of iron, copper, and zinc, also we measured the concentration of paraquat in cerebellum, and proinflammatory cytokines in serum and cerebellum. The effect on paraquat-treated mice with 5 mg/kg and principal component analysis of iron showed suggestive quantitative trait loci on chromosome 5. Overall, our results suggest that gene Prkag2 and related networks may serve as potential targets against paraquat toxicity and demonstrate the utility of genetically diverse mouse models for the study of complex human toxicity.

scholarly journals Improved Strain Distribution Patterns of SMXA Recombinant Inbred Strains by Microsatellite Markers

2003 ◽  
Vol 52 (5) ◽  
pp. 415-417 ◽  
Author(s):  
Tamio OHNO ◽  
Jun-ichi KATOH ◽  
Yoshiaki KIKKAWA ◽  
Hiromichi YONEKAWA ◽  
Masahiko NISHIMURA
Keyword(s):  
Microsatellite Markers ◽  
Recombinant Inbred ◽  
Strain Distribution ◽  
Distribution Patterns ◽  
Inbred Strains ◽  
Recombinant Inbred Strains ◽  
Improved Strain
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