scholarly journals Structure and Expression of Myelin Basic Protein Gene Sequences in the mld Mutant Mouse: Reiteration and Rearrangement of the MBP Gene

Genetics ◽  
1987 ◽  
Vol 116 (3) ◽  
pp. 447-464
Author(s):  
Alfred A Akowitz ◽  
Elisa Barbarese ◽  
Kathy Scheld ◽  
John H Carson
Keyword(s):  
Myelin Basic Protein ◽  
Structural Gene ◽  
Basic Protein ◽  
Primary Cultures ◽  
Gene Sequences ◽  
Cis Acting ◽  
Steady State Levels ◽  
Myelin Basic Protein Gene ◽  
The Brain ◽  
Specific Mrna

ABSTRACT The mld mutation on chromosome 18 in the mouse is a putative allele of the shiverer (shi) mutation. We have analyzed the structure of myelin basic protein (MBP) gene sequences in mld DNA by restriction mapping of genomic DNA. The results indicate that the mld chromosome carries two copies of the MBP structural gene, one of which is intact and one of which is interrupted. Genetic analysis indicates that the interrupted gene is close to the intact MBP structural gene and cosegregates with the mld mutation. We have also analyzed the levels of MBP polypeptides and MBP-specific mRNA in wild-type, homozygous and heterozygous shiverer and mld mice and in mice carrying both mutations. The results indicate that both shi and mld are cis-acting codominant mutations that cause severely reduced steady state levels of MBP-specific mRNA and MBP polypeptides in the brain. We have analyzed the total number of oligodendrocytes and the number of MBP-positive oligodendrocytes in mld and shibrain primary cultures. In shi cultures, none of the oligodendrocytes expresses MBP. However, in mld cultures, approximately 5% of the oligodendrocytes express MBP. The nature of the "revertant" mld oligodendrocytes is not known.

scholarly journals Expression of myelin basic protein gene in the developing rat brain as revealed by in situ hybridization.

1986 ◽  
Vol 34 (4) ◽  
pp. 467-473 ◽  
Author(s):  
K Kristensson ◽  
N K Zeller ◽  
M E Dubois-Dalcq ◽  
R A Lazzarini
Keyword(s):  
In Situ Hybridization ◽  
Myelin Basic Protein ◽  
Basic Protein ◽  
Seventh Cranial Nerve ◽  
Myelin Basic Protein Gene ◽  
Old Rats ◽  
Developing Rat ◽  
The Brain ◽  
Specific Mrna

The developmental program controlling the expression of myelin basic protein (MBP) gene was studied in the rat using the technique of in situ hybridization. A 35S-labeled cDNA clone of mouse MBP encoding an amino acid sequence present in all four of the major forms of rodent MBP was used. The probe hybridized to the tracts of white matter with different intensities, depending on the age of the animal and the region of the brain examined. In the medulla oblongata, maximal hybridization was found in 5- and 7-day-old rats and was confined to the tectospinal tracts, fibers of the seventh cranial nerve, and the spinocerebellar tracts. By 12 days the amount of MBP mRNA had decreased in these areas. In the cerebrum, the greatest amount of MBP mRNA was observed in 17-day-old rats in the radiations of the corpus callosum. Thereafter, the levels decreased but could still be observed in the adult animals. Thus, using this technique, we have been able to demonstrate that the level of MBP-specific mRNA correlates closely with the development of myelin in different regions of the brain.

Escherichia coli phenylalanyl-tRNA synthetase operon: transcription studies of wild-type and mutated operons on multicopy plasmids

1982 ◽  
Vol 152 (2) ◽  
pp. 661-668
Author(s):  
J A Plumbridge ◽  
M Springer
Keyword(s):  
Structural Gene ◽  
Trna Synthetase ◽  
Mrna Levels ◽  
Structural Genes ◽  
Cis Acting ◽  
Trna Synthetases ◽  
Hybridization Probes ◽  
Steady State Levels ◽  
Derivatives Of

The construction of three lambda bacteriophages containing parts of the structural gene for threonyl-tRNA synthetase, thrS, and those for the two subunits of phenylalanyl-tRNA synthetases, pheS and pheT, is described. These phages were used as hybridization probes to measure the in vivo levels of mRNA specific to these three genes. Plasmid pB1 carries the three genes thrS, pheS, and pheT, and strains carrying the plasmid show enhanced levels of mRNA corresponding to these genes. Although the steady-state levels of threonyl-tRNA synthetase and phenylalanyl-tRNA synthetase produced by the presence of the plasmid differed by a factor of 10, their pulse-labeled mRNA levels were about the same. Mutant derivatives of pB1 were also analyzed. Firstly, a cis-acting insertion located before the structural genes for phenylalanyl-tRNA synthetase caused a major decrease in both pheS and pheT mRNA. Secondly, mutations affecting either structural gene pheS or pheT caused a reduction in the mRNA levels for both pheS and pheT. This observation suggests that autoregulation plays a role in the expression of phenylalanyl-tRNA synthetase.

Myelin basic protein gene-related protein (MGRP)

1992 ◽  
Vol 17 ◽  
pp. 139
Author(s):  
Mitsuko Sugano ◽  
Tatsunori Seki ◽  
Hiroki Nakae
Keyword(s):  
Myelin Basic Protein ◽  
Basic Protein ◽  
Protein Gene ◽  
Related Protein ◽  
Myelin Basic Protein Gene

Linkage mapping of the porcine myelin basic protein gene to chromosome 11

2005 ◽  
Vol 36 (2) ◽  
pp. 163-164
Author(s):  
J. G. Kim ◽  
D. Nonneman ◽  
J. L. Vallet ◽  
G. A. Rohrer ◽  
R. K. Christenson
Keyword(s):  
Myelin Basic Protein ◽  
Linkage Mapping ◽  
Basic Protein ◽  
Protein Gene ◽  
Chromosome 11 ◽  
Myelin Basic Protein Gene
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