scholarly journals FINE STRUCTURE MAPPING OF THE am (GDH) LOCUS OF NEUROSPORA

Genetics ◽  
1983 ◽  
Vol 105 (2) ◽  
pp. 293-307
Author(s):  
John A Rambosek ◽  
John A Kinsey
Keyword(s):  
Fine Structure ◽  
Deletion Mapping ◽  
Structure Mapping ◽  
Marker Analysis

ABSTRACT Utilizing a combination of flanking marker analysis and deletion mapping we have constructed a fine structure map of the am locus which includes 63 point mutants and ten unique deletions. Positions of point mutants can be rapidly assigned to one of 13 segments within the gene on the basis of crosses to nine deletion strains.

scholarly journals A COMPARISON OF TWO-POINT, THREE-POINT AND DELETION MAPPING IN THE C CISTRON OF RHIZOBIOPHAGE 16–3, WITH AN EXPLANATION FOR THE RECOMBINATION PATTERN

Genetics ◽  
1980 ◽  
Vol 94 (2) ◽  
pp. 249-263
Author(s):  
LÁszlÓ Orosz ◽  
Katalin RostÁs ◽  
Rollin D Hotchkiss
Keyword(s):  
Fine Structure ◽  
High Frequency ◽  
Rhizobium Meliloti ◽  
General Tendency ◽  
Deletion Mapping ◽  
Structure Mapping ◽  
Mapping Data ◽  
Correct Order ◽  
Point Mapping ◽  
Double Crossovers

ABSTRACT A general tendency for additivity prevailed in recombination frequencies for two-point fine-structure mapping of 14 mutants in the C cistron of Rhizobium meliloti phage 16-3, with little evidence of any marker effect. Intracistronic three-point mapping indicated that double crossovers are rare. Deletion mapping indicated that the two- and three-point mapping data gave the correct order of the mutations. A high frequency (5 to 8%) of c/c+ heterozygotic phage progenies was observed in standard crosses. This pattern implies formation of a relatively long region of heterozygosity. Together with the results of the three-point tests, it suggests certain properties of the branch migration and resolution steps envisioned in current mechanisms of recombination.

Mutations at the Saccharomyces cerevisiae SUP4 tRNA Tyr Locus: Isolation, Genetic Fine-Structure Mapping, and Correlation with Physical Structure

1982 ◽  
Vol 2 (12) ◽  
pp. 1501-1513
Author(s):  
Janet Kurjan ◽  
Benjamin D. Hall
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Fine Structure ◽  
Physical Structure ◽  
Mapping Technique ◽  
Structure Mapping ◽  
Isolation And Characterization ◽  
Qualitative Estimate ◽  
Genetic Fine Structure ◽  
Entire Gene

The SUP4 tRNA Tyr locus in Saccharomyces cerevisiae has been studied by the isolation and characterization of mutations at the SUP4 gene which result in the loss of suppressor function. Most of the mutations act as single-site mutations, whereas about a third of the mutations are deletions of the entire gene. Two meiotic fine-structure maps of the gene were made. The first mapping technique placed 10 mutations plus the sup4 + anticodon on a map by a measurement of levels of recombination between pairs of mutations. The second map utilized a more qualitative estimate of recombination frequency, allowing 69 mutations and the sup4 + anticodon to be mapped. The maps were compared with the physical structure of the gene for the 34 mutations whose nucleotide alteration has been determined by DNA sequencing (Koski et al., Cell 22 :415-425, 1980; Kurjan et al., Cell 20 :701-709, 1980). Both maps show a good correlation with the physical structure of the gene, even though certain properties of genetic fine-structure maps, such as marker effects and “map expansion,” were seen.

scholarly journals A CIS-DOMINANT MUTATION IN ASPERGILLUS NIDULANS AFFECTING THE EXPRESSION OF THE amdS GENE IN THE PRESENCE OF MUTATIONS IN THE UNLINKED GENE, amdA

Genetics ◽  
1982 ◽  
Vol 102 (2) ◽  
pp. 139-147
Author(s):  
Michael J Hynes
Keyword(s):  
Fine Structure ◽  
Genetic Analysis ◽  
Genetic Mapping ◽  
Gene Function ◽  
The Other ◽  
Structure Mapping ◽  
Dominant Mutation ◽  
Amds Gene ◽  
Very High ◽  
New Mutations

ABSTRACT A mutant producing very high levels of the acetamidase enzyme encoded by the amdS gene has been isolated in a strain containing the amdA7 mutation, which itself causes high levels of this enzyme. Genetic analysis has shown that this mutation, designated amdI66, is adjacent to the amdS gene and is cis-dominant in its effect. The amdI66 mutation has little effect on amdS expression when present in strains not containing the amdA7 mutation. Two other amdA mutations investigated also interact with the amdI66 mutation to result in high acetamidase levels. No interaction between amdI66 and any of the other putative regulatory genes affecting amdS expression has been observed. The amdI66 mutation has been located by fine structure mapping at the extreme end of the controlling region, which has previously been defined by genetic mapping (Hynes 1979). Analysis of this region has been extended by mapping new mutations resulting in loss of amdS expression. One of these defines the most extreme site capable of mutation to loss of gene function found so far.

Fine structure mapping of CIAS1: identification of an ancestral haplotype and a common FCAS mutation, L353P

2003 ◽  
Vol 112 (2) ◽  
pp. 209-216 ◽  
Author(s):  
Hal M. Hoffman ◽  
Simon G. Gregory ◽  
James L. Mueller ◽  
Mark Tresierras ◽  
David H. Broide ◽  
...  
Keyword(s):  
Fine Structure ◽  
Ancestral Haplotype ◽  
Structure Mapping

Fine-structure mapping of the complex locus Odc-rs5 relative to Igk and distal loci

1992 ◽  
Vol 3 (12) ◽  
pp. 689-699 ◽  
Author(s):  
Beverly A. Richards-Smith ◽  
Rosemary W. Elliott
Keyword(s):  
Fine Structure ◽  
Structure Mapping ◽  
Complex Locus
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