scholarly journals Functional Genomics of a Symbiotic Community: Shared Traits in the Olive Fruit Fly Gut Microbiota

2019 ◽  
Vol 12 (2) ◽  
pp. 3778-3791 ◽  
Author(s):  
Frances Blow ◽  
Anastasia Gioti ◽  
Ian B Goodhead ◽  
Maria Kalyva ◽  
Anastasia Kampouraki ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Transcriptome Assembly ◽  
Indirect Evidence ◽  
Fruit Fly ◽  
Underlying Mechanism ◽  
Olive Fruit Fly ◽  
Olive Fruit ◽  
Dietary Nitrogen ◽  
Obligate Symbiont ◽  
Insect Gut

Abstract The olive fruit fly Bactrocera oleae is a major pest of olives worldwide and houses a specialized gut microbiota dominated by the obligate symbiont “Candidatus Erwinia dacicola.” Candidatus Erwinia dacicola is thought to supplement dietary nitrogen to the host, with only indirect evidence for this hypothesis so far. Here, we sought to investigate the contribution of the symbiosis to insect fitness and explore the ecology of the insect gut. For this purpose, we examined the composition of bacterial communities associated with Cretan olive fruit fly populations, and inspected several genomes and one transcriptome assembly. We identified, and reconstructed the genome of, a novel component of the gut microbiota, Tatumella sp. TA1, which is stably associated with Mediterranean olive fruit fly populations. We also reconstructed a number of pathways related to nitrogen assimilation and interactions with the host. The results show that, despite variation in taxa composition of the gut microbial community, core functions related to the symbiosis are maintained. Functional redundancy between different microbial taxa was observed for genes involved in urea hydrolysis. The latter is encoded in the obligate symbiont genome by a conserved urease operon, likely acquired by horizontal gene transfer, based on phylogenetic evidence. A potential underlying mechanism is the action of mobile elements, especially abundant in the Ca. E. dacicola genome. This finding, along with the identification, in the studied genomes, of extracellular surface structure components that may mediate interactions within the gut community, suggest that ongoing and past genetic exchanges between microbes may have shaped the symbiosis.

scholarly journals Functional genomics of a symbiotic community: shared traits in the olive fruit fly gut microbiota

2019 ◽  
Author(s):  
Frances Blow ◽  
Anastasia Gioti ◽  
Ian B. Goodhead ◽  
Maria Kalyva ◽  
Anastasia Kampouraki ◽  
...  
Keyword(s):  
Gut Microbiota ◽  
Indirect Evidence ◽  
Fruit Fly ◽  
Underlying Mechanism ◽  
Olive Fruit Fly ◽  
Olive Fruit ◽  
Dietary Nitrogen ◽  
Obligate Symbiont ◽  
Insect Gut ◽  
Insect Fitness

AbstractThe olive fruit flyBactroceraoleae is a major pest of olives worldwide and houses a specialized gut microbiota dominated by the obligate symbiont “CandidatusErwinia dacicola”.Ca. E. dacicola is thought to supplement dietary nitrogen to the host, with only indirect evidence for this hypothesis so far. Here, we sought to investigate the contribution of the symbiosis to insect fitness and explore the ecology of the insect gut. For this purpose, we examined the composition of bacterial communities associated with Cretan olive fruit fly populations, and inspected several genome and transcriptome assemblies. We identified, and reconstructed the genome of, a novel component of the gut microbiota,Tatumellasp. TA1, which is stably associated with Mediterranean olive fruit fly populations. We also reconstructed a number of pathways related to nitrogen assimilation and interaction with the host. The results show that, despite variation in taxa composition of the gut microbial community, core functions related to the symbiosis are maintained. Functional redundancy between different microbial taxa was observed for genes allowing urea hydrolysis. The latter is encoded in the obligate symbiont genome by a conserved urease operon, likely acquired by horizontal gene transfer, based on phylogenetic evidence. A potential underlying mechanism is the action of mobile elements, especially abundant in theCa. E. dacicola genome. This finding, along with the identification, in the studied genomes, of extracellular surface structure components that may mediate interactions within the gut community, suggest that ongoing and past genetic exchanges between microbes may have shaped the symbiosis.

scholarly journals Review of the role of gut microbiota in mass rearing of the olive fruit fly,Bactrocera oleae, and its parasitoids

2017 ◽  
Vol 164 (3) ◽  
pp. 237-256 ◽  
Author(s):  
Erica Ras ◽  
Leo W. Beukeboom ◽  
Carlos Cáceres ◽  
Kostas Bourtzis
Keyword(s):  
Gut Microbiota ◽  
Fruit Fly ◽  
Mass Rearing ◽  
Bactrocera Oleae ◽  
Olive Fruit Fly ◽  
Olive Fruit

scholarly journals Olive fruit fly and its obligate symbiont Candidatus Erwinia dacicola: Two new symbiont haplotypes in the Mediterranean basin

PLoS ONE ◽  
2021 ◽  
Vol 16 (9) ◽  
pp. e0256284
Author(s):  
Tânia Nobre
Keyword(s):  
Mediterranean Basin ◽  
Olive Tree ◽  
Fruit Fly ◽  
Population Diversity ◽  
Olive Fruit Fly ◽  
Direct Production ◽  
Olive Fruit ◽  
Obligate Symbiont ◽  
The Mediterranean ◽  
The Mediterranean Basin

The olive fruit fly, specialized to become monophagous during several life stages, remains the most important olive tree pest with high direct production losses, but also affecting the quality, composition, and inherent properties of the olives. Thought to have originated in Africa is nowadays present wherever olive groves are grown. The olive fruit fly evolved to harbor a vertically transmitted and obligate bacterial symbiont -Candidatus Erwinia dacicola- leading thus to a tight evolutionary history between olive tree, fruit fly and obligate, vertical transmitted symbiotic bacterium. Considering this linkage, the genetic diversity (at a 16S fragment) of this obligate symbiont was added in the understanding of the distribution pattern of the holobiont at nine locations throughout four countries in the Mediterranean Basin. This was complemented with mitochondrial (four mtDNA fragments) and nuclear (ten microsatellites) data of the host. We focused on the previously established Iberian cluster for the B. oleae structure and hypothesised that the Tunisian samples would fall into a differentiated cluster. From the host point of view, we were unable to confirm this hypothesis. Looking at the symbiont, however, two new 16S haplotypes were found exclusively in the populations from Tunisia. This finding is discussed in the frame of host-symbiont specificity and transmission mode. To understand olive fruit fly population diversity and dispersion, the dynamics of the symbiont also needs to be taken into consideration, as it enables the fly to, so efficiently and uniquely, exploit the olive fruit resource.

scholarly journals Transcriptome landscape of the developing olive fruit fly embryo delineated by Oxford Nanopore long-read RNA-Seq

2018 ◽  
Author(s):  
Anthony Bayega ◽  
Spyros Oikonomopoulos ◽  
Eleftherios Zorbas ◽  
Yu Chang Wang ◽  
Maria-Eleni Gregoriou ◽  
...  
Keyword(s):  
Embryo Development ◽  
De Novo ◽  
Transcriptome Assembly ◽  
Fruit Fly ◽  
Full Length ◽  
Olive Fruit Fly ◽  
Rna Seq ◽  
Olive Fruit ◽  
Olive Fly ◽  
Oxford Nanopore

AbstractThe olive fruit fly or olive fly (Bactrocera oleae) is the most important pest of cultivated olive trees. Like all insects the olive fly undergoes complete metamorphosis. However, the transcription dynamics that occur during early embryonic development have not been explored, while detailed transcriptomic analysis in the absence of a fully annotated genome is challenging. We collected olive fly embryos at hourly intervals for the first 6 hours of development and performed full-length cDNA-Seq using a purpose designed SMARTer cDNA synthesis protocol followed by sequencing on the MinION (Oxford Nanopore Technologies). We generated 31 million total reads across the timepoints (median yield 4.2 million per timepoint). The reads showed 98 % alignment rate to the olive fly genome and 91 % alignment rate to the NBCI predicted B. oleae gene models. Over 50 % of the expressed genes had at least one read covering its entire length validating our full-length RNA-Seq procedure. Expression of 68 % of the predicted B. oleae genes was detected in the first six hours of development. We generated a de novo transcriptome assembly of the olive fly and identified 3553 novel genes and a total of 79,810 transcripts; a fourfold increase in transcriptome diversity compared to the NCBI predicted transcriptome. On a global scale, the first six hours of embryo development were characterized by dramatic transcriptome changes with the total number of transcripts per embryo dropping to half from the first hour to the second hour of embryo development. Clustering of genes based on temporal co-expression followed by gene-set enrichment analysiss of genes expressed in the first six hours of embryo development showed that genes involved in transcription and translation, macro-molecule biosynthesis, and neurodevelopment were highly enriched. These data provide the first insight into the transcriptome landscape of the developing olive fly embryo. The data also reveal transcript signatures of sex development. Overall, full-length sequencing of the cDNA molecules permitted a detailed characterization of the isoform complexity and the transcriptional dynamics of the first embryonic stages of the B. oleae.

Dynamics of oil quality parameters changes related to olive fruit fly attack

2010 ◽  
Vol 112 (9) ◽  
pp. 1033-1040 ◽  
Author(s):  
Olivera Koprivnjak ◽  
Ivana Dminić ◽  
Urška Kosić ◽  
Valerija Majetić ◽  
Sara Godena ◽  
...  
Keyword(s):  
Oil Quality ◽  
Fruit Fly ◽  
Quality Parameters ◽  
Olive Fruit Fly ◽  
Olive Fruit

scholarly journals Stochastic Modeling and Simulation of Olive Fruit Fly Outbreaks

2013 ◽  
Vol 8 ◽  
pp. 580-586 ◽  
Author(s):  
Spyros Voulgaris ◽  
Michalis Stefanidakis ◽  
Andreas Floros ◽  
Markos Avlonitis
Keyword(s):  
Modeling And Simulation ◽  
Stochastic Modeling ◽  
Fruit Fly ◽  
Olive Fruit Fly ◽  
Olive Fruit
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