scholarly journals A Phylogenomic Approach to Clarifying the Relationship of Mesodinium within the Ciliophora: A Case Study in the Complexity of Mixed-Species Transcriptome Analyses

2019 ◽  
Vol 11 (11) ◽  
pp. 3218-3232
Author(s):  
Erica Lasek-Nesselquist ◽  
Matthew D Johnson

Abstract Recent high-throughput sequencing endeavors have yielded multigene/protein phylogenies that confidently resolve several inter- and intra-class relationships within the phylum Ciliophora. We leverage the massive sequencing efforts from the Marine Microbial Eukaryote Transcriptome Sequencing Project, other SRA submissions, and available genome data with our own sequencing efforts to determine the phylogenetic position of Mesodinium and to generate the most taxonomically rich phylogenomic ciliate tree to date. Regardless of the data mining strategy, the multiprotein data set, or the molecular models of evolution employed, we consistently recovered the same well-supported relationships among ciliate classes, confirming many of the higher-level relationships previously identified. Mesodinium always formed a monophyletic group with members of the Litostomatea, with mixotrophic species of Mesodinium—M. rubrum, M. major, and M. chamaeleon—being more closely related to each other than to the heterotrophic member, M. pulex. The well-supported position of Mesodinium as sister to other litostomes contrasts with previous molecular analyses including those from phylogenomic studies that exploited the same transcriptomic databases. These topological discrepancies illustrate the need for caution when mining mixed-species transcriptomes and indicate that identifying ciliate sequences among prey contamination—particularly for Mesodinium species where expression from stolen prey nuclei appears to dominate—requires thorough and iterative vetting with phylogenies that incorporate sequences from a large outgroup of prey.

Author(s):  
Nguyen Thu Ha ◽  
Nguyen Thi Thanh Huyen

The retail market in Vietnam continues to grow with the entry of foreign retail brands and the strong rise of domestic businesses in expanding distribution networks and conquering consumer confidence. The appearance of more retail brands has created a fiercely competitive market. Based on the outcomes of previous research results on brand choice intention combined with a customer survey, the paper proposes an analytical framework and scales to examine the relationship of five elements including store image, price perception, risk perception, brand attitudes, brand awareness and retail brand choice intention with a case study of the Hanoi-based Circle K convenience store chain. These five elements are the precondition for retail businesses to develop their brands so as to attract customers.


2018 ◽  
Vol 2 (2) ◽  
pp. 115
Author(s):  
Taufik Abrain

Several studies have shown that the success of interregional cooperation may be influenced by coordination, commitment, participation, variance of cooperation, structure, format of cooperation, and political will. Nevertheless, these factors do not stand alone since actor relations as a determining aspect is capable of driving those factors effectively. This article aims to examine the aspect of actor relations as a contributing factor that determines successful cooperation among regions. This is a qualitative research with the policy of inter-regional cooperation of the Banjarbakula Program, South Kalimantan Province from February 2017 to February 2018, set as its object of study. The result of this study states that the success of inter-regional cooperation is influenced by the relationship of actors in development factors as suggested by previous experts. The actors involved in the inter-regional cooperation examined in this case had become triggers of coordination, commitment, and participation toward success and failure, as well as the effectiveness of regional cooperation policy. Structural obstacles, ego-centric character, minimum budget availability, and non-visionary planning could be overcome as long as actor relations were properly managed.


Genetics ◽  
1997 ◽  
Vol 146 (3) ◽  
pp. 995-1010 ◽  
Author(s):  
Rafael Zardoya ◽  
Axel Meyer

The complete nucleotide sequence of the 16,407-bp mitochondrial genome of the coelacanth (Latimeria chalumnae) was determined. The coelacanth mitochondrial genome order is identical to the consensus vertebrate gene order which is also found in all ray-finned fishes, the lungfish, and most tetrapods. Base composition and codon usage also conform to typical vertebrate patterns. The entire mitochondrial genome was PCR-amplified with 24 sets of primers that are expected to amplify homologous regions in other related vertebrate species. Analyses of the control region of the coelacanth mitochondrial genome revealed the existence of four 22-bp tandem repeats close to its 3′ end. The phylogenetic analyses of a large data set combining genes coding for rRNAs, tRNA, and proteins (16,140 characters) confirmed the phylogenetic position of the coelacanth as a lobe-finned fish; it is more closely related to tetrapods than to ray-finned fishes. However, different phylogenetic methods applied to this largest available molecular data set were unable to resolve unambiguously the relationship of the coelacanth to the two other groups of extant lobe-finned fishes, the lungfishes and the tetrapods. Maximum parsimony favored a lungfish/coelacanth or a lungfish/tetrapod sistergroup relationship depending on which transversion:transition weighting is assumed. Neighbor-joining and maximum likelihood supported a lungfish/tetrapod sistergroup relationship.


2017 ◽  
Vol 49 (3) ◽  
pp. 382-399 ◽  
Author(s):  
JOSHUA BERNING ◽  
ADAM N. RABINOWITZ

AbstractWe examine the relationship of product characteristics of ready-to-eat breakfast cereal and targeted television advertising to specific consumer segments. We compile a unique data set that includes brand-packaging characteristics, including on-box games, nutrition information, and cobranding. We find that the relationship of television advertising and a cereal's brand-packaging characteristics varies by target audience. Our results provide insight into understanding how manufacturers strategically utilize branding, packaging, and television advertising. This can help industry and policy makers develop food product advertising policy. This analysis extends to other product markets where extensive product differentiation and promotion are present as well.


2013 ◽  
Vol 3 (4) ◽  
pp. 128 ◽  
Author(s):  
Seema Qureshi

Managers play an important role in organizations. They are the persons who formulate and implement policies, plans etc in the organization. Managers are the first to be contacted for suggestions with regard to overall information about the organization. Taking into consideration the importance of managers in the organization and a critical review of literature which revealed that the study on work motivation, burnout and intention to leave has not been conducted so far on the top level managers of garment industry of Delhi and NCR, the researcher found a gap to be filled by  conducting investigation on this sample for the variables in question. So the sample of the present study consists of top level managers of garment industry of Delhi and NCR. Thus, a convenient sampling method was used for drawing the sample from 60 garment export houses of Delhi and 40 of NCR. This paper gives a deep insight of work motivation, burnout and intention to leave for the top level managers of the garment industry of Delhi/NCR. In this paper we have checked the relationship of all dimensions of work motivation with the dimensions of burnout and intention to leave and have observed that all dimensions of work motivation have some more or less relationship with each dimension of burnout but neither of the dimensions of work motivation or total work motivation have any relationship with intention to leave. So even if top level managers leave their current job they don’t leave because of lack of work motivation.


2021 ◽  
pp. 78-107
Author(s):  
Lizeth Benavides ◽  
Natasha Cabrera_Jara ◽  
Belén Campoverde_Bermeo

El cambio de modelo urbano asumido durante el siglo XX, trajo un sinnúmero de problemas como la priorización del vehículo, por lo que en la última década han surgido esfuerzos para dotar de importancia al ciudadano de a pie, en el espacio público. Esta investigación estudió las condiciones físico-espaciales de un corredor urbano donde el modelo centrado en el vehículo se acentúa, con la fnalidad de generar posibles estrategias que reviertan esta situación. Se tomó como caso de estudio a la Av. 24 de Mayo, en Azogues, y se lo analizó mediante una metodología mixta, que evaluó, detalladamente, tres zonas de estudio, determinando que la falta de accesibilidad y conectividad y el modelo de movilidad defendido por la ciudadanía, en general, infuyen directamente en las condiciones del espacio público peatonal y por ende en la habitabilidad urbana, perjudicando los desplazamientos a pie. Palabras clave: Espacio público; habitabilidad urbana; conectividad; accesibilidad; percepción. AbstractThe change of urban model assumed during the 20th century, brought countless problems such as the prioritization of vehicles, so in the last decade eforts have emerged to give importance to the citizen on foot in the public space. This original research studied the relationship of urban habitability with the physical-spatial conditions of an urban corridor, where the vehicle-centered model is accentuated, to generate possible strategies to reverse this situation. The Av. 24 de Mayo in Azogues was taken as a case study and analyzed using a mixed methodology that evaluated in detail three study areas, determining that the lack of accessibility and connectivity and the mobility model defended by citizens in general have a direct infuence on the conditions of the pedestrianpublic space and, therefore, on urban habitability, which afects walking Keywords: Public space; urban habitability; connectivity; accessibility; perception.


10.28945/3041 ◽  
2006 ◽  
Author(s):  
Karlheinz Kautz ◽  
Bjarke Nielsen

Information systems development takes place within an economical context. However, the economical conditions, which shape systems development in practice, are hardly researched. In this paper we are investigating the question how a given price structure influences systems development projects. Our analysis is based on a multi case study and a Grounded Theory inspired research approach. Our work is informed by economic theories, which deal with the relationship of suppliers and customers and their mutual dependency. We thus apply principal-agent theory and economic game theory in form of the prisoner’s dilemma. As a result we provide three interlinked models for understanding the impact of pricing structure on systems development practice. The main elements of these models are pricing structure, risk distribution and price level, and opportunistic behavior. We discuss how these elements are interrelated and examine their impact on information systems development in practice.


2019 ◽  
Vol 3 (1) ◽  
pp. 81-113
Author(s):  
Ulfatun Nisa ◽  
Fitriyah Mahdali

This study focuses on the interconnection and the integration, namely about the relationship of, education and Alquran. This study is a case study that took Pondok Pesantren Al-Aqobah Diwek Jombang as the research location. There are several arguments that form the basis of this research, firstly, this pesantren is applying both the salafiyah boarding school system and the modern system of education of technology and science. Secondly, santris in this pesantren are required to memorize hadis and Alquran using the principle of one day five verses and one hadis. In a matter of months, santris in this pesantren have studied the science of religion which counts generally should be studied in annual time. This research is a qualitative-explorative study that uses the theory of interconnected-integrative paradigm. In accordance with the research theory, the formulation of the problem taken to conduct this research is “what is the paradigm of Quranic based education and how is it implemented in Pondok Pesantren Al-Aqobah”. The methods of data collection are documentation, observation, and interview. The findings of this study are: (1) Educational and Social Institutions of Pondok Pesantren Al-Aqobah Jombang is an educational institution that offers various programs to develop the potential of santri or students using Alquran as the basis for their education; (2) it uses the MIR (Multiple Intelligence Research) method as an effort to explore various kinds of children's abilities, and so that their potencies can be maximized.


2020 ◽  
Vol 4 (2) ◽  
pp. 94-125
Author(s):  
Mohammed Jarallah Tewfik ◽  
Ahmed Mohammed Yahya Al-Abbasi

The interior design of Malaysian Islamic mosques is a vivid example of further innovation and innovation based on design creativity in design relationships.As a result of the interaction of this level, the design process shows a clear independence as a result of the interaction of the subjective capabilities factor of the interior designer based on the study of the specificity of the objective capabilities that govern the processes of drafting innovative decorative arts.Therefore, it was necessary to study this issue by identifying the research problem, which is summarized by: showing the features of design independence as a complementary principle in the designs of the interior spaces of Malaysian mosques,While the aim of the research focuses on identifying the features of design independence that are adopted as a complementary principle in the designs of the interior spaces of Malaysian mosques,While the importance of research is evident in presenting a clear picture of the concept of design independence, as it represents the theoretical base that can be used in practical application in designs of interior spaces for the chapel of the Malaysian Islamic mosques,The research study also includes both (research limits, theoretical framework, as well as research procedures based on the descriptive analytical approach (content analysis - case study)) leading to the results of the research study, which was among the most important: 1- Design configurations of all kinds and design configurations emerged within the designs of the internal determinants, based on the study of the interior designer, to the aesthetic independence of the division of space and size as a complementary principle within the internal determinants of the mosque of the two mosques. 2- The relationship of the principle of the independence of convergence as a complementary principle contributed to determining the distances between shapes, which can be perceived as a unified whole, through the distribution of units and shapes within a consecutive visual design system. While the most important recommendations emerged through the necessity of studying the choice of levels of internal determinants of the chapel of the Malaysian Islamic mosques.With the demonstration of the expressive characteristic of civilization development through design recruitment of appropriate vocabulary and design units with a careful selection of modern materials and materials in line with the innovative design path to achieve the requirements of design independence within the chapel of the Malaysian Islamic mosques.


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