scholarly journals Evolutionary Relatedness and Classification of Tumor-Inducing and Opine-Catabolic Plasmids in Three Rhizobium rhizogenes Strains Isolated from the Same Crown Gall Tumor

2019 ◽  
Vol 11 (6) ◽  
pp. 1525-1540 ◽  
Author(s):  
Nemanja Kuzmanović ◽  
Joanna Puławska
Keyword(s):  
Crown Gall ◽  
Crown Gall Tumor ◽  
Evolutionary Relatedness ◽  
Rhizobium Rhizogenes ◽  
Catabolic Plasmids

scholarly journals Cumulative Cytotoxicity Assay of the Aqueous and Ethanolic Extracts of the Selected Medicinal Plants using Crown Gall Tumor Disc Bioassay

2020 ◽  
Author(s):  
Sandeep Waghulde ◽  
Tushar Baviskar ◽  
Praful Patil ◽  
Shweta Singh ◽  
Nilesh Gorde ◽  
...  
Keyword(s):  
Medicinal Plants ◽  
Crown Gall ◽  
Cytotoxicity Assay ◽  
Crown Gall Tumor ◽  
Ethanolic Extracts

N6-(Δ2-isopentenyl)adenosine from crown gall tumor tissue of Vinca rosea

1976 ◽  
Vol 15 (10) ◽  
pp. 1565 ◽  
Author(s):  
Chong-Maw Chen ◽  
Richard L. Eckert ◽  
James D. McChesney
Keyword(s):  
Crown Gall ◽  
Tumor Tissue ◽  
Crown Gall Tumor ◽  
Vinca Rosea ◽  
Isopentenyl Adenosine

scholarly journals Studies on the recovery of crown gall tumor cells

1976 ◽  
Vol 73 (10) ◽  
pp. 3562-3564 ◽  
Author(s):  
R. Turgeon ◽  
H. N. Wood ◽  
A. C. Braun
Keyword(s):  
Tumor Cells ◽  
Crown Gall ◽  
Crown Gall Tumor

scholarly journals Grapevine (Vitis vinifera) Crown Galls Host Distinct Microbiota

2016 ◽  
Vol 82 (18) ◽  
pp. 5542-5552 ◽  
Author(s):  
Hanna Faist ◽  
Alexander Keller ◽  
Ute Hentschel ◽  
Rosalia Deeken
Keyword(s):  
Microbial Community ◽  
Crown Gall ◽  
Amplicon Sequencing ◽  
Agrobacterium Vitis ◽  
Graft Union ◽  
Content Type ◽  
Crown Gall Tumor ◽  
Crown Gall Disease ◽  
Opportunistic Bacteria

ABSTRACTCrown gall disease of grapevine is caused by virulentAgrobacteriumstrains and establishes a suitable habitat for agrobacteria and, potentially, other bacteria. The microbial community associated with grapevine plants has not been investigated with respect to this disease, which frequently results in monetary losses. This study compares the endophytic microbiota of organs from grapevine plants with or without crown gall disease and the surrounding vineyard soil over the growing seasons of 1 year. Amplicon-based community profiling revealed that the dominating factor causing differences between the grapevine microbiota is the sample site, not the crown gall disease. The soil showed the highest microbial diversity, which decreased with the distance from the soil over the root and the graft union of the trunk to the cane. Only the graft union microbiota was significantly affected by crown gall disease. The bacterial community of graft unions without a crown gall hosted transient microbiota, with the three most abundant bacterial species changing from season to season. In contrast, graft unions with a crown gall had a higher species richness, which in every season was dominated by the same three bacteria (Pseudomonassp.,Enterobacteriaceaesp., andAgrobacterium vitis). Forin vitro-cultivated grapevine plantlets,A. vitisinfection alone was sufficient to cause crown gall disease. Our data show that microbiota in crown galls is more stable over time than microbiota in healthy graft unions and that the microbial community is not essential for crown gall disease outbreak.IMPORTANCEThe characterization of bacterial populations in animal and human diseases using high-throughput deep-sequencing technologies, such as 16S amplicon sequencing, will ideally result in the identification of disease-specific microbiota. We analyzed the microbiota of the crown gall disease of grapevine, which is caused by infection with the bacterial pathogenAgrobacterium vitis.All otherAgrobacteriumspecies were found to be avirulent, even though they lived together withA. vitisin the same crown gall tumor. As has been reported for human cancer, the crown gall tumor also hosted opportunistic bacteria that are adapted to the tumor microenvironment. Characterization of the microbiota in various diseases using amplicon sequencing may help in early diagnosis, to serve as a preventative measure of disease in the future.

scholarly journals Unusual plasmid DNA organization in an octopine crown gall tumor

1985 ◽  
Vol 13 (2) ◽  
pp. 459-471 ◽  
Author(s):  
William W. Kwok ◽  
Eugene W. Nester
Keyword(s):  
Plasmid Dna ◽  
Crown Gall ◽  
Crown Gall Tumor ◽  
Dna Organization
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