Three new genome assemblies support a rapid radiation in Musa acuminata (wild banana)

Deteksi Awal Ketahanan Beberapa Aksesi Pisang Lokal dan Hasil Persilangan terhadap Penyakit Layu Fusarium (Foc VCG 01213/16 TR4)

Jurnal Hortikultura Indonesia ◽

10.29244/jhi.8.2.88-96 ◽

2018 ◽

Vol 8 (2) ◽

pp. 88

Author(s):

Tri Handayani ◽

Diyah Martanti ◽

Yuyu S. Poerba ◽

Witjaksono ,

Keyword(s):

Early Detection ◽

Fusarium Oxysporum ◽

Fusarium Wilt ◽

Field Condition ◽

Musa Acuminata ◽

Greenhouse Condition ◽

Wild Banana

ABSTRACTEarly detection for Fusarium wilt resistant of banana plants can be done at seedling phase under greenhouse condition. The purpose of early detection was to screen and evaluate some number of local and hybrid accession of banana plants for resistance to Fusarium oxysporum f. sp. cubense (Foc VCG 01213/16 TR4 or Foc TR4). The treatments were done by immersing the root seedling in Fusarium inoculant at concentration of 106 conidia mL-1, for 2 hours, then planted in sterile sand media and placed in container with "double tray system". Experiments were performed using 15 accessions of banana i.e. 1 accession of wild musa (Musa acuminata var. malaccesis), 7 local accessions and 7 hybrid bananas. Based on greenhouse bioassay, banana Rejang#2 (AA) showed resistance to Foc TR4. The results were consistent in the field condition. Tetraploid plants for pisang Rejang#2 (AAAA) also showed resistant to Foc TR4. Cultivar Cavendish (AAA) and Madu (AA) showed highly susceptible; Ustrali (AAAB) and Goroho (AAA) showed susceptible to Foc TR4. Wild banana (Musa acuminata var. malaccensis) and Mas Jambe 4x (AAAA) showed moderate susceptible. Of the total 7 hybrids accession; 3 hybrids showed moderate susceptible, and 4 other hybrids showed susceptible to Foc TR4.Keywords: banana plants, early detection, Foc TR4, hybrid, local ABSTRAK Deteksi awal tingkat ketahananan tanaman pisang terhadap penyakit layu Fusarium oxysporum f. sp. cubense (Foc) dapat dilakukan pada masa pembibitan di rumah kaca. Penelitian bertujuan untuk evaluasi awal tingkat ketahanan beberapa aksesi pisang liar, pisang lokal dan pisang hasil persilangan terhadap penyakit layu Fusarium. Akar bibit pisang pada air yang berisi inokulan Fusarium (Foc VCG 01213/16 atau Foc TR4) pada konsentrasi 106 konidia mL-1, selama 2 jam yang selanjutnya ditanam pada media pasir steril dan diletakkan pada kontainer dengan pengaturan “double tray system”. Lima belas aksesi pisang yang terdiri atas 1 aksesi pisang liar (Musa acuminata var. malaccensis), 7 aksesi pisang budidaya dan 7 nomor pisang hasil persilangan dievaluasi tingkat ketahanan terhadap Foc TR4. Dari hasil pengujian di rumah kaca, pisang Rejang#2 (AA) menunjukkan status tahan terhadap penyakit layu Fusarium Foc VCG 01213/16 TR4 yang hasilnya sesuai dan stabil dengan tingkat ketahanannya di lapangan. Pisang Rejang#2 tetraploid (AAAA) juga menunjukkan status tahan terhadap Foc TR4. Pisang Madu (AA) dan Cavendish (AAA) menunjukkan hasil sangat rentan, sedangkan pisang Goroho (AAA) dan pisang Ustrali (AAAB) menunjukkan status rentan. Pisang liar Musa acuminata var. malaccensis (AA) dan pisang Mas Jambe 4x (AAAA) menunjukkan status agak rentan. Pada seleksi pisang hasil persilangan, didapatkan 3 nomor pisang hibrid dengan status agak rentan yakni 816 MDRK, 1060 MDRK, 2284 MMRK, sedangkan 4 nomor hibrid lainnya menunjukkan status rentan.Kata kunci: deteksi dini, Foc TR4, hibrid, lokal, pisang

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MORPHOLOGICAL CHARACTERIZATION OF MALAYSIAN WILD BANANA MUSA ACUMINATA

BIOTROPIA ◽

10.11598/btb.2002.0.18.170 ◽

2002 ◽

Vol 0 (18) ◽

Keyword(s):

Morphological Characterization ◽

Musa Acuminata ◽

Wild Banana

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Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing

Communications Biology ◽

10.1038/s42003-021-02559-3 ◽

2021 ◽

Vol 4 (1) ◽

Author(s):

Caroline Belser ◽

Franc-Christophe Baurens ◽

Benjamin Noel ◽

Guillaume Martin ◽

Corinne Cruaud ◽

...

Keyword(s):

Musa Acuminata ◽

Genetic Maps ◽

Nanopore Sequencing ◽

Genome Coverage ◽

Long Reads ◽

Oxford Nanopore ◽

A Genome ◽

Long Read ◽

Genome Assemblies ◽

First Time

AbstractLong-read technologies hold the promise to obtain more complete genome assemblies and to make them easier. Coupled with long-range technologies, they can reveal the architecture of complex regions, like centromeres or rDNA clusters. These technologies also make it possible to know the complete organization of chromosomes, which remained complicated before even when using genetic maps. However, generating a gapless and telomere-to-telomere assembly is still not trivial, and requires a combination of several technologies and the choice of suitable software. Here, we report a chromosome-scale assembly of a banana genome (Musa acuminata) generated using Oxford Nanopore long-reads. We generated a genome coverage of 177X from a single PromethION flowcell with near 17X with reads longer than 75 kbp. From the 11 chromosomes, 5 were entirely reconstructed in a single contig from telomere to telomere, revealing for the first time the content of complex regions like centromeres or clusters of paralogous genes.

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Telomere-to-telomere gapless chromosomes of banana using nanopore sequencing

10.1101/2021.04.16.440017 ◽

2021 ◽

Author(s):

Caroline Belser ◽

Franc-Christophe Baurens ◽

Benjamin Noel ◽

Guillaume Martin ◽

Corinne Cruaud ◽

...

Keyword(s):

Musa Acuminata ◽

Genetic Maps ◽

Nanopore Sequencing ◽

Genome Coverage ◽

Long Reads ◽

Oxford Nanopore ◽

A Genome ◽

Long Read ◽

Genome Assemblies ◽

First Time

AbstractLong-read technologies hold the promise to obtain more complete genome assemblies and to make them easier. Coupled with long-range technologies, they can reveal the architecture of complex regions, like centromeres or rDNA clusters. These technologies also make it possible to know the complete organization of chromosomes, which remained complicated before even when using genetic maps. However, generating a gapless and telomere-to-telomere assembly is still not trivial, and requires a combination of several technologies and the choice of suitable software. Here, we report a chromosome-scale assembly of a banana genome (Musa acuminata) generated using Oxford Nanopore long-reads. We generated a genome coverage of 177X from a single PromethION flowcell with near 17X with reads longer than 75Kb. From the 11 chromosomes, 5 were entirely reconstructed in a single contig from telomere to telomere, revealing for the first time the content of complex regions like centromeres or clusters of paralogous genes.

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Endophytic Fungi from Wild Banana (Musa acuminata Colla) Works Against Anthracnose Disease Caused by Colletotrichum musae

Research Journal of Microbiology ◽

10.3923/jm.2008.368.374 ◽

2008 ◽

Vol 3 (5) ◽

pp. 368-374 ◽

Cited By ~ 7

Author(s):

W. Nuangmek . ◽

E.H.C. McKenzie . ◽

S. Lumyong .

Keyword(s):

Endophytic Fungi ◽

Musa Acuminata ◽

Anthracnose Disease ◽

Wild Banana ◽

Colletotrichum Musae

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Haplotype network analysis of wild banana relatives Ensete glaucum, Musa acuminata and Musa balbisiana based on cpDNA rbcL sequences in ex-situ collection

Indian Journal of Genetics and Plant Breeding (The) ◽

10.31742/ijgpb.80.3.9 ◽

2020 ◽

Vol 80 (03) ◽

Author(s):

Lia Hapsari ◽

Dewi Ayu Lestari ◽

Rasyadan Taufiq Probojati

Keyword(s):

Network Analysis ◽

Nucleotide Diversity ◽

Single Point ◽

Haplotype Diversity ◽

Haplotype Network ◽

Musa Acuminata ◽

Ex Situ ◽

Single Point Mutation ◽

Rbcl Gene ◽

Wild Banana

Conservation and genetic assessment of wild banana relatives is important for future breeding purposes. Haplotype network analysis was conducted to wild banana relatives comprised Ensete glaucum, Musa acuminata and Musa balbisianausing rbcL gene sequences. Sequences characterization showed high conservation level (91%), low indels (1.83%), and low parsimony informatives (3.51%). However, it was powerfull to separate the dataset at families, genera and species level; and moderately to separate at intraspecies level of wild bananas. Haplotype and nucleotide diversity of wild bananas were high. At intraspecies level, both M. acuminata and M. balbisiana showed high haplotype diversity but low nucelotide diversity among haplotypes; M. acuminata has higher value than M. balbisiana. No haplotype and nucleotide diversity in E. glaucum. Wild bananas were separated into seven haplotypes, with four haplogroups. Mutational pathway revealed that E. glaucum haplotype became root; and was closely related to M. balbisiana than M. acuminata. M. acuminata var. malaccensis haplotype became root within all M. acuminata varieties, and each haplotype differed by single point mutation.

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Kultur Embrio Pisang Liar Musa acuminata ssp. sumatrana yang Langka (Embryo Culture of Endangered Wild Banana Musa acuminata ssp. sumatrana)

Jurnal Hortikultura ◽

10.21082/jhort.v28n1.2018.p25-32 ◽

2019 ◽

Vol 28 (1) ◽

pp. 25

Author(s):

Ika Roostika ◽

Agus Sutanto ◽

NFN Edison ◽

Nurwita Dewi

Keyword(s):

Growth Rate ◽

Survival Rate ◽

Combined Treatment ◽

Shoot Multiplication ◽

Musa Acuminata ◽

In Vitro Conservation ◽

Plant Materials ◽

Randomized Design ◽

Wild Banana

Musa acuminata ssp. sumatrana adalah pisang liar yang langka dan perlu dilestarikan. Teknik kultur in vitro dapat diterapkan untuk melestarikannya. Penerapan teknik konservasi secara in vitro memerlukan penguasaan metode regenerasi, termasuk formulasi media tumbuh. Penelitian bertujuan mengetahui pengaruh BA, TDZ, dan PVP terhadap daya hidup dan regenerasi embrio M. acuminata ssp. sumatrana. Rancangan percobaan disusun secara faktorial dalam lingkungan rancangan acak lengkap dengan empat ulangan. Faktor pertama adalah BA (0, 1, 3, dan 5 mg/L), faktor kedua adalah TDZ (0 dan 0,1 mg/L) dan faktor ketiga adalah PVP (100 dan 300 mg/L). Peubah yang diamati adalah persentase daya hidup, persentase daya tumbuh, persentase pembentukan akar, jumlah tunas, jumlah akar, dan jumlah daun yang terbentuk. Hasil penelitian menunjukkan terdapat interaksi yang nyata antara taraf BA, TDZ, dan PVP terhadap seluruh peubah yang diamati. Kombinasi perlakuan BA 5 mg/L dengan TDZ 0,1 mg/L dan PVP 300 mg/L menghasilkan persentase hidup, persentase tumbuh, jumlah tunas, dan jumlah daun yang paling tinggi, berturut-turut 100% daya hidup, 100% daya tumbuh, lima tunas/eksplan, dan 15 daun/tunas. Media tersebut dapat diterapkan untuk perbanyakan in vitro M. acuminata ssp. sumatrana dalam penyediaan materi untuk konservasi in vitro.KeywordsMusa acuminata ssp. sumatrana; Pisang liar langka; Regenerasi embrioAbstractMusa acuminata ssp. sumatrana is an endangered wild banana species that should be conserved. The in vitro culture can be applied for conserving wild banana accessions. The establishment of regeneration method, including the use of growth medium, is required in the application of in vitro conservation. The influence of BA, TDZ, and PVP to the survival and growth rate of embryos of M. acuminata ssp. sumatrana will be discussed in this study. The factorial in compeletely randomized design with four replications was used in this study. The first factor was BA (0, 1, 3, and 5 mg/L), the second factor was TDZ (0 and 0.1 mg/L), and the third factor was PVP (100 and 300 mg/L). Variables observed were the percentage of survival rate, the percentage of growth rate, the percentage of root formation, number of shoot, root, and leaf. The result a significant interaction between the concentration of BA, TDZ, and PVP to all observed variables. The combined treatment between 5 mg/L BA, 0.1 mg/L TDZ, and 300 mg/L PVP provided the highest survival rate (100%), growth rate (100%), shoot multiplication (five shoots/explant), and number of leaf (15 leaves/shoot). This medium can be applied for micropropagation of M. acuminata ssp. sumatrana in supplying plant materials for in vitro conservation.

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- Diversity wild banana species (Musa spp.) in Sulawesi, Indonesia

Biodiversitas Journal of Biological Diversity ◽

10.13057/biodiv/d200328 ◽

2019 ◽

Vol 20 (3) ◽

pp. 824-832 ◽

Cited By ~ 1

Author(s):

HASTUTI HASTUTI ◽

PURNOMO PURNOMO ◽

I SUMARDI ◽

BUDI S DARYONO

Keyword(s):

Endemic Species ◽

Musa Acuminata ◽

Residential Areas ◽

Musa Balbisiana ◽

Purposive Sampling ◽

Musa Spp ◽

The World ◽

Wild Banana

Abstract. Hastuti, Purnomo, Sumardi I, Daryono BS. 2019. Diversity wild banana species (Musa spp.) in Sulawesi, Indonesia. Biodiversitas 20: 824-832. Indonesia is known as one of the centers of banana diversity in the world. There are 70 species of wild banana in the genus of Musa, 12 of them it was found in Indonesia. Sulawesi was located in the Wallace Line, which is a meeting between the Sunda and Sahul exposures, and has many endemic species, some of which are wild banana species. However, studies on wild banana in Sulawesi are still limited. This study focuses on species of wild bananas found in Sulawesi. Purposive sampling was used to select banana example from wild, cultivated area, residential areas and research garden. Observation was carried out in the field as well as using a collection herbarium and living specimens. The observation was done by observing the characteristics of the sample according to the guidance on Descriptor for Banana. Identification was done by matching the sample with herbarium and relevant references. The results showed that there were four species of wild bananas found on the island of Sulawesi. These are Musa balbisiana Colla, Musa acuminata Colla var. zebrina (v.Houtte) Nasution, Musa acuminata Colla var. banksii (F.Muell.) N.W Simmonds, Musa acuminata Colla var. lutraensis, Musa acuminata Colla var. sigiensis, Musa acuminata Colla ssp. microcarpa Becc., Musa borneensis Becc. and Musa textilis Nee. Four wild banana accessions had never been reported before. These were Musa acuminata var. zebrina, Musa acuminata var. lutraensis, Musa acuminata var. siginenesis and Musa borneensis.

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