scholarly journals Hellbender Genome Sequences Shed Light on Genomic Expansion at the Base of Crown Salamanders

2014 ◽  
Vol 6 (7) ◽  
pp. 1818-1829 ◽  
Author(s):  
C. Sun ◽  
R. L. Mueller
Keyword(s):  
Genome Sequences ◽  
Shed Light ◽  
Genomic Expansion

scholarly journals New complete genome sequences of human rhinoviruses shed light on their phylogeny and genomic features

BMC Genomics ◽  
2007 ◽  
Vol 8 (1) ◽  
pp. 224 ◽  
Author(s):  
Caroline Tapparel ◽  
Thomas Junier ◽  
Daniel Gerlach ◽  
Samuel Cordey ◽  
Sandra Van Belle ◽  
...  
Keyword(s):  
Complete Genome ◽  
Genome Sequences ◽  
Genomic Features ◽  
Shed Light

scholarly journals Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins

2020 ◽  
pp. mcp.RA120.002295 ◽  
Author(s):  
Yong Zhang ◽  
Wanjun Zhao ◽  
Yonghong Mao ◽  
Yaohui Chen ◽  
Shisheng Wang ◽  
...  
Keyword(s):  
Insect Cells ◽  
Host Cells ◽  
Protein Subunit ◽  
Genome Sequences ◽  
Complex Type ◽  
Site Specific ◽  
Differential Processing ◽  
S Protein ◽  
Shed Light

The glycoprotein spike (S) on the surface of SARS-CoV-2 is a determinant for viral invasion and host immune response. Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides. All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences. The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunit. We identified masses that correspond to 157 N-glycans, primarily of the complex type. In contrast, the insect cell-expressed S protein contained 38 N-glycans, completely of the high-mannose type. Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells, regardless of the glycosites’ location. Moreover, the N-glycan compositions were conserved among different sizes of subunits. Our study indicate that the S protein N-glycosylation occurs regularly at each site, albeit the occupied N-glycans were diverse and heterogenous. This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.

Whole-genome sequences shed light onto the demographic history and contemporary genetic erosion of free-ranging jaguar (Panthera onca) populations

2021 ◽  
Author(s):  
Gustavo P. Lorenzana ◽  
Henrique V. Figueiró ◽  
Christopher B. Kaelin ◽  
Gregory S. Barsh ◽  
Jeremy Johnson ◽  
...  
Keyword(s):  
Demographic History ◽  
Genetic Erosion ◽  
Whole Genome ◽  
Panthera Onca ◽  
Genome Sequences ◽  
Free Ranging ◽  
Shed Light

scholarly journals Site-specific N-glycosylation Characterization of Recombinant SARS-CoV-2 Spike Proteins

2020 ◽  
Author(s):  
Yong Zhang ◽  
Wanjun Zhao ◽  
Yonghong Mao ◽  
Yaohui Chen ◽  
Shisheng Wang ◽  
...  
Keyword(s):  
Insect Cells ◽  
Host Cells ◽  
Genome Sequences ◽  
Complex Type ◽  
Site Specific ◽  
Differential Processing ◽  
S Protein ◽  
Protein Subunits ◽  
Shed Light

SummaryThe glycoprotein spike (S) on the surface of SARS-CoV-2 is a determinant for viral invasion and host immune response. Herein, we characterized the site-specific N-glycosylation of S protein at the level of intact glycopeptides. All 22 potential N-glycosites were identified in the S-protein protomer and were found to be preserved among the 753 SARS-CoV-2 genome sequences. The glycosites exhibited glycoform heterogeneity as expected for a human cell-expressed protein subunits. We identified masses that correspond to 157 N-glycans, primarily of the complex type. In contrast, the insect cell-expressed S protein contained 38 N-glycans, primarily of the high-mannose type. Our results revealed that the glycan types were highly determined by the differential processing of N-glycans among human and insect cells. This N-glycosylation landscape and the differential N-glycan patterns among distinct host cells are expected to shed light on the infection mechanism and present a positive view for the development of vaccines and targeted drugs.

scholarly journals Draft Genome Sequences of Two Pseudomonas Strains That Are Able To Use Furan Derivatives as Their Sole Carbon Source

2020 ◽  
Vol 9 (2) ◽  
Author(s):  
Carlos Farkas ◽  
Raúl A. Donoso ◽  
Carla Gárate-Castro ◽  
Pamela Villegas ◽  
Roberto E. Durán ◽  
...  
Keyword(s):  
Wastewater Treatment ◽  
Carbon Source ◽  
Sole Carbon Source ◽  
Draft Genome ◽  
Genome Sequences ◽  
Catabolic Pathways ◽  
Furan Derivatives ◽  
Treatment Facilities ◽  
Shed Light

Pseudomonas sp. strains ALS1279 and ALS1131 were isolated from wastewater treatment facilities on the basis of their ability to use furfural, a key lignocellulose-derived inhibitor, as their only carbon source. Here, we present the draft genome sequences of both strains, which can shed light on catabolic pathways for furan compounds in pseudomonads.

scholarly journals Halocins, Bacteriocin-Like Antimicrobials Produced by the Archaeal Domain: Occurrence and Phylogenetic Diversity in Halobacteriales

2020 ◽  
Author(s):  
Afef Najjari ◽  
Hiba Mejri ◽  
Marwa Jabbari ◽  
Haitham Sghaier ◽  
Ameur Cherif ◽  
...  
Keyword(s):  
Amino Acids ◽  
Phylogenetic Analysis ◽  
Amino Acid ◽  
Complete Genome ◽  
Halophilic Archaea ◽  
Amino Acid Sequences ◽  
Genome Sequences ◽  
Encoding Gene ◽  
New Research ◽  
Shed Light

Members of extremely halophilic archaea, currently consisting of more than 56 genera and 216 species, are known to produce their specific bacteriocin-like peptides and proteins called halocins, synthesized by the ribosomal pathway. Halocins are diverse in size, consisting of proteins as large as 35 kDa and peptide “microhalocins” as small as 3.6 kDa. Today, about fifteen halocins have been described and only three genes, halC8, halS8 and halH4, coding C8, S8 and H4 halocins respectively have been identified. In this study, a total of 1858 of complete and nearly complete genome sequences of Halobacteria class members were retrieved from the IMG and Genbank databases and then screened for halocin encoding gene content, based on the BLASTP algorithm. A total of 61 amino acid sequences belonging to three halocins classes (C8, HalH4 and S8) were identified within 15 genera with the abundance of C8 class. Phylogenetic analysis based on amino acids sequences showed a clear segregation of the three halocins classes. Halocin S8 was phylogenetically more close to HalH4. No clear segregation on species and genera levels was observed based on halocin C8 analysiscontrary to HalH4 based analysis. Collectively, these results give an overview on halocins diversity within halophilic archaea which can open new research topics that will shed light on halocins as marker for haloarchaeal phylogentic delineation.

Elucidating cyclic AMP signaling in subcellular domains with optogenetic tools and fluorescent biosensors

2019 ◽  
Vol 47 (6) ◽  
pp. 1733-1747 ◽  
Author(s):  
Christina Klausen ◽  
Fabian Kaiser ◽  
Birthe Stüven ◽  
Jan N. Hansen ◽  
Dagmar Wachten
Keyword(s):  
Cyclic Amp ◽  
Adenosine Monophosphate ◽  
Adenylyl Cyclases ◽  
Temporal Precision ◽  
Fluorescent Biosensors ◽  
Subcellular Domains ◽  
Spatio Temporal ◽  
Hydrolysis Of ◽  
Shed Light ◽  
Cyclic Amp Signaling

The second messenger 3′,5′-cyclic nucleoside adenosine monophosphate (cAMP) plays a key role in signal transduction across prokaryotes and eukaryotes. Cyclic AMP signaling is compartmentalized into microdomains to fulfil specific functions. To define the function of cAMP within these microdomains, signaling needs to be analyzed with spatio-temporal precision. To this end, optogenetic approaches and genetically encoded fluorescent biosensors are particularly well suited. Synthesis and hydrolysis of cAMP can be directly manipulated by photoactivated adenylyl cyclases (PACs) and light-regulated phosphodiesterases (PDEs), respectively. In addition, many biosensors have been designed to spatially and temporarily resolve cAMP dynamics in the cell. This review provides an overview about optogenetic tools and biosensors to shed light on the subcellular organization of cAMP signaling.

Sentence-Based Experience Logging in New Hearing Aid Users

2020 ◽  
Vol 29 (3S) ◽  
pp. 631-637
Author(s):  
Katja Lund ◽  
Rodrigo Ordoñez ◽  
Jens Bo Nielsen ◽  
Dorte Hammershøi
Keyword(s):  
Hearing Aid ◽  
Patient Experiences ◽  
Online Tool ◽  
Rehabilitation Process ◽  
Hearing Rehabilitation ◽  
Clinical Observations ◽  
Hearing Aid Use ◽  
Wide Range ◽  
Insight Into ◽  
Shed Light

Purpose The aim of this study was to develop a tool to gain insight into the daily experiences of new hearing aid users and to shed light on aspects of aided performance that may not be unveiled through standard questionnaires. Method The tool is developed based on clinical observations, patient experiences, expert involvement, and existing validated hearing rehabilitation questionnaires. Results An online tool for collecting data related to hearing aid use was developed. The tool is based on 453 prefabricated sentences representing experiences within 13 categories related to hearing aid use. Conclusions The tool has the potential to reflect a wide range of individual experiences with hearing aid use, including auditory and nonauditory aspects. These experiences may hold important knowledge for both the patient and the professional in the hearing rehabilitation process.

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