scholarly journals The Genome of Setaria digitata: A Cattle Nematode Closely Related to Human Filarial Parasites

2020 ◽  
Vol 12 (2) ◽  
pp. 3971-3976
Author(s):  
Kanchana S Senanayake ◽  
Jonas Söderberg ◽  
Aleksei Põlajev ◽  
Maja Malmberg ◽  
Eric H Karunanayake ◽  
...  
Keyword(s):  
Developing Countries ◽  
Lymphatic Filariasis ◽  
Genome Sequence ◽  
Drug Targets ◽  
Parasitic Nematode ◽  
Draft Genome ◽  
Model Organism ◽  
Wuchereria Bancrofti ◽  
Setaria Digitata ◽  
Novel Drugs

Abstract Here we present the draft genome sequence of Setaria digitata, a parasitic nematode affecting cattle. Due to its similarity to Wuchereria bancrofti, the parasitic nematode that causes lymphatic filariasis in humans, S. digitata has been used as a model organism at the genomic level to find drug targets which can be used for the development of novel drugs and/or vaccines for human filariasis. Setaria digitata causes cerebrospinal nematodiasis in goats, sheep, and horses posing a serious threat to livestock in developing countries. The genome sequence of S. digitata will assist in finding candidate genes to use as drug targets in both S. digitata and W. bancrofti. The assembled draft genome is ∼90 Mb long and contains 8,974 genomic scaffolds with a G+C content of 31.73%.

scholarly journals Construction of Metabolic Pathways based on Whole Genome Sequencing Reveals Laterally- Transferred Wolbachia-like Sequences in the Setaria Digitata Genome

2021 ◽  
Author(s):  
Nadarajah Rashanthy ◽  
Mahil Sharada Alwis Kothalawala ◽  
Thamarahansi Shashiprabha Mugunamalwaththa ◽  
Kasun de Zoysa ◽  
Naduviladath Vishvanath Chandrasekharan ◽  
...  
Keyword(s):  
Metabolic Pathways ◽  
Functional Annotation ◽  
Sequence Similarity ◽  
Draft Genome ◽  
Model Organism ◽  
Animal Husbandry ◽  
Wuchereria Bancrofti ◽  
Economic Losses ◽  
Whole Genome ◽  
Setaria Digitata

Abstract Setaria digitata is a Wolbachia-free filarial parasite that causes cerebrospinal nematodiasis in non-permissive hosts such as goats, sheep and horses leading to substantial economic losses in animal husbandry. Due to its similarity to Wuchereria bancrofti, primary causing agent of human lymphatic filariasis (HLF), S. digitata can be used as a model organism to study the biology of HLF. This study was mainly aimed to bring functional analysis of metabolic pathways in S. digitata. A draft genome of 78,774,594 bases making a total of 2,075 contigs was generated. ‘BLAST2GO’ functional annotation resulted in 28112 BLAST hits with an e-value lower than 1e-4 and a sequence similarity higher than 30%. Out of a total of 2075 contigs, 1280 contigs were used to generate a total of 6055 GO annotations at a mean level of 6.488 with standard deviation of 2.675. Overall, 89.1% of mapped reads were annotated by at least one of the three categories of the GO function classification. Moreover, 111 enzymes associated with 95 distinct metabolic pathways were identified. We suggest that S. digitata may have evolved its own sequences to code for haem, riboflavin, and FAD in the absence of Wolbachia.

Setaria digitata in advancing our knowledge of human lymphatic filariasis

2015 ◽  
Vol 90 (2) ◽  
pp. 129-138 ◽  
Author(s):  
A.N.I. Perumal ◽  
Y.I.N.S. Gunawardene ◽  
R.S. Dassanayake
Keyword(s):  
Lymphatic Filariasis ◽  
Model Organism ◽  
Animal Husbandry ◽  
Wuchereria Bancrofti ◽  
Filarial Parasite ◽  
Economic Losses ◽  
Setaria Digitata ◽  
Close Resemblance ◽  
The Tropics ◽  
Control And Eradication

AbstractSetaria digitata is a filarial parasite that causes fatal cerebrospinal nematodiasis in goats, sheep and horses, resulting in substantial economic losses in animal husbandry in the tropics. Due to its close resemblance to Wuchereria bancrofti, this nematode is also frequently used as a model organism to study human lymphatic filariasis. This review highlights numerous insights into the morphological, histological, biochemical, immunological and genetic aspects of S. digitata that have broadened our understanding towards the control and eradication of filarial diseases.

Draft genome sequence of Sinorhizobium meliloti RU11/001, a model organism for flagellum structure, motility and chemotaxis

2013 ◽  
Vol 168 (4) ◽  
pp. 731-733 ◽  
Author(s):  
Daniel Wibberg ◽  
Jochen Blom ◽  
Christian Rückert ◽  
Anika Winkler ◽  
Andreas Albersmeier ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Sinorhizobium Meliloti ◽  
Draft Genome ◽  
Model Organism ◽  
Draft Genome Sequence

scholarly journals Draft genome sequence of the Wolbachia endosymbiont of Wuchereria bancrofti wWb

2017 ◽  
Vol 75 (9) ◽  
Author(s):  
Matthew Chung ◽  
Scott T Small ◽  
David Serre ◽  
Peter A Zimmerman ◽  
Julie C Dunning Hotopp
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Wuchereria Bancrofti ◽  
Draft Genome Sequence ◽  
Wolbachia Endosymbiont

scholarly journals Draft Genome Sequence ofAcinetobacter johnsoniiMB44, Exhibiting Nematicidal Activity againstCaenorhabditis elegans

2016 ◽  
Vol 4 (1) ◽  
Author(s):  
Shijing Tian ◽  
Muhammad Ali ◽  
Li Xie ◽  
Lin Li
Keyword(s):  
Caenorhabditis Elegans ◽  
Molecular Mechanism ◽  
Genome Sequence ◽  
Plant Tissue ◽  
Tissue Sample ◽  
Draft Genome ◽  
Model Organism ◽  
Nematicidal Activity ◽  
Draft Genome Sequence ◽  
Acinetobacter Johnsonii

Acinetobacter johnsoniiMB44 was isolated from a frost-plant-tissue sample, which showed noteworthy nematicidal activity against the model organismCaenorhabditis elegans. Here, we report the 3.4 Mb draft genome ofA. johnsoniiMB44, which will help in understanding the molecular mechanism of its ability to infect nematodes.

scholarly journals Draft Genome Sequence of Myxococcus xanthus Wild-Type Strain DZ2, a Model Organism for Predation and Development

2013 ◽  
Vol 1 (3) ◽  
Author(s):  
S. Muller ◽  
J. W. Willett ◽  
S. M. Bahr ◽  
C. L. Darnell ◽  
K. R. Hummels ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Type Strain ◽  
Wild Type Strain ◽  
Myxococcus Xanthus ◽  
Draft Genome ◽  
Model Organism ◽  
Draft Genome Sequence ◽  
Wild Type

scholarly journals Draft Genome Sequence of Serratia proteamaculans MFPA44A14-05, a Model Organism for the Study of Meat and Seafood Spoilage

2017 ◽  
Vol 5 (23) ◽  
Author(s):  
Lysiane Fougy ◽  
Gwendoline Coeuret ◽  
Marie-Christine Champomier-Vergès ◽  
Stéphane Chaillou
Keyword(s):  
Genome Sequence ◽  
Draft Genome ◽  
Model Organism ◽  
Draft Genome Sequence ◽  
Modified Atmosphere ◽  
Content Type ◽  
Serratia Proteamaculans

ABSTRACT In this study, we present a draft genome sequence of Serratia proteamaculans MFPA44A14-05. This strain was isolated from a spoiled organic modified-atmosphere-packed beef carpaccio. The draft genome sequence will contribute to the understanding of the role of the S. proteamaculans species in meat and seafood spoilage.

scholarly journals Draft Genome Sequence ofAgreia bicolorataStrain AC-1804, a Producer of Large Amounts of Carotenoid Pigments, Isolated from Narrow Reed Grass Infected by the Phytoparasitic Nematode

2015 ◽  
Vol 3 (6) ◽  
Author(s):  
Ilya Vasilyev ◽  
Maria Siniagina ◽  
Sergey Malanin ◽  
Eugenia Boulygina ◽  
Tatiana Grygoryeva ◽  
...  
Keyword(s):  
Genome Sequence ◽  
Parasitic Nematode ◽  
Draft Genome ◽  
Plant Parasitic Nematode ◽  
Draft Genome Sequence ◽  
Carotenoid Pigments ◽  
Phytoparasitic Nematode ◽  
Plant Parasitic ◽  
Carotenoid Pathway

Here, we report the draft genome sequence ofAgreia bicoloratastrain AC-1804, isolated from narrow reed grass galls induced by a plant-parasitic nematode which is able to produce large amounts of carotenoid pigments. The draft genome sequence of 3,919,485 bp provides a resource for carotenoid pathway research.

scholarly journals Human Migration and the Spread of the Nematode Parasite Wuchereria bancrofti

2019 ◽  
Vol 36 (9) ◽  
pp. 1931-1941 ◽  
Author(s):  
Scott T Small ◽  
Frédéric Labbé ◽  
Yaya I Coulibaly ◽  
Thomas B Nutman ◽  
Christopher L King ◽  
...  
Keyword(s):  
Lymphatic Filariasis ◽  
Drug Targets ◽  
Population Genomics ◽  
Wuchereria Bancrofti ◽  
Human Migration ◽  
Human Populations ◽  
Asian Origin ◽  
Demographic Models ◽  
Whole Genomes ◽  
Timing Of Migration

Abstract The human disease lymphatic filariasis causes the debilitating effects of elephantiasis and hydrocele. Lymphatic filariasis currently affects the lives of 90 million people in 52 countries. There are three nematodes that cause lymphatic filariasis, Brugia malayi, Brugia timori, and Wuchereria bancrofti, but 90% of all cases of lymphatic filariasis are caused solely by W. bancrofti (Wb). Here we use population genomics to reconstruct the probable route and timing of migration of Wb strains that currently infect Africa, Haiti, and Papua New Guinea (PNG). We used selective whole genome amplification to sequence 42 whole genomes of single Wb worms from populations in Haiti, Mali, Kenya, and PNG. Our results are consistent with a hypothesis of an Island Southeast Asia or East Asian origin of Wb. Our demographic models support divergence times that correlate with the migration of human populations. We hypothesize that PNG was infected at two separate times, first by the Melanesians and later by the migrating Austronesians. The migrating Austronesians also likely introduced Wb to Madagascar where later migrations spread it to continental Africa. From Africa, Wb spread to the New World during the transatlantic slave trade. Genome scans identified 17 genes that were highly differentiated among Wb populations. Among these are genes associated with human immune suppression, insecticide sensitivity, and proposed drug targets. Identifying the distribution of genetic diversity in Wb populations and selection forces acting on the genome will build a foundation to test future hypotheses and help predict response to current eradication efforts.

Sign in / Sign up

Export Citation Format

Share Document