The Influence of Desiccation, Rough Handling and Cold Storage on the Quality and Establishment of Sitka Spruce Planting Stock

1990 ◽  
Vol 63 (2) ◽  
pp. 129-141 ◽  
Author(s):  
J. D. DEANS ◽  
C. LUNDBERG ◽  
P. M. TABBUSH ◽  
M. G. R. CANNELL ◽  
L. J. SHEPPARD ◽  
...  
1992 ◽  
Vol 49 (1-2) ◽  
pp. 101-107 ◽  
Author(s):  
J.D. Deans ◽  
W.L. Mason ◽  
F.J. Harvey

1980 ◽  
Vol 4 (4) ◽  
pp. 188-189 ◽  
Author(s):  
L. Eric Hinesley ◽  
Mikal E. Saltviet

Abstract Fall lifting of nursery stock may entail cold storage in facilities which contain high levels of ethylene due to the presence of apples. Three-year-old (3-0) Fraser fir (Abies fraseri (Pursh) Poir.) seedlings continuously exposed to 17.5 ppm ethylene in air during 8 weeks of cold storage at 38°F produced 22 percent less terminal growth than untreated seedlings during the first growing season after transplanting. There was no effect on the number of terminal buds which aborted or which grew abnormally following transplanting in a line-out bed.


2000 ◽  
Vol 57 (2) ◽  
pp. 101-111 ◽  
Author(s):  
Didier Garriou ◽  
Sabine Girard ◽  
Jean-Marc Guehl ◽  
Beno�t G�n�r�

Author(s):  
Henry H. Eichelberger ◽  
John G. Baust ◽  
Robert G. Van Buskirk

For research in cell differentiation and in vitro toxicology it is essential to provide a natural state of cell structure as a benchmark for interpreting results. Hypothermosol (Cryomedical Sciences, Rockville, MD) has proven useful in insuring the viability of synthetic human epidermis during cold-storage and in maintaining the epidermis’ ability to continue to differentiate following warming.Human epidermal equivalent, EpiDerm (MatTek Corporation, Ashland, MA) consisting of fully differentiated stratified human epidermal cells were grown on a microporous membrane. EpiDerm samples were fixed before and after cold-storage (4°C) for 5 days in Hypothermosol or skin culture media (MatTek Corporation) and allowed to recover for 7 days at 37°C. EpiDerm samples were fixed 1 hour in 2.5% glutaraldehyde in sodium cacodylate buffer (pH 7.2). A secondary fixation with 0.2% ruthenium tetroxide (Polysciences, Inc., Warrington, PA) in sodium cacodylate was carried out for 3 hours at 4°C. Other samples were similarly fixed, but with 1% Osmium tetroxide in place of ruthenium tetroxide. Samples were dehydrated through a graded acetone series, infiltrated with Spurrs resin (Polysciences Inc.) and polymerized at 70°C.


1919 ◽  
Vol 87 (2269supp) ◽  
pp. 404-405
Author(s):  
L. A. Welo
Keyword(s):  

Sign in / Sign up

Export Citation Format

Share Document