scholarly journals Signature-tagged mutagenesis and co-infection studies demonstrate the importance of P fimbriae in a murine model of urinary tract infection

2015 ◽  
Vol 73 (4) ◽  
Author(s):  
Eric L. Buckles ◽  
Courtney L. Luterbach ◽  
Xiaolin Wang ◽  
C. Virginia Lockatell ◽  
David E. Johnson ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Murine Model

scholarly journals Impact of Host Age and Parity on Susceptibility to Severe Urinary Tract Infection in a Murine Model

PLoS ONE ◽  
2014 ◽  
Vol 9 (5) ◽  
pp. e97798 ◽  
Author(s):  
Kimberly A. Kline ◽  
Drew J. Schwartz ◽  
Nicole M. Gilbert ◽  
Amanda L. Lewis
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Murine Model ◽  
Host Age

scholarly journals Struvite Urolithiasis and Chronic Urinary Tract Infection in a Murine Model of Urinary Diversion

Urology ◽  
2013 ◽  
Vol 81 (5) ◽  
pp. 943-948 ◽  
Author(s):  
Brian Becknell ◽  
Ashley R. Carpenter ◽  
Brad Bolon ◽  
John R. Asplin ◽  
Susan E. Ingraham ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Urinary Diversion ◽  
Murine Model ◽  
Chronic Urinary Tract Infection

scholarly journals A murine model of urinary tract infection

2009 ◽  
Vol 4 (8) ◽  
pp. 1230-1243 ◽  
Author(s):  
Chia-Suei Hung ◽  
Karen W Dodson ◽  
Scott J Hultgren
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Murine Model

scholarly journals Impact of Low-Level Resistance to Fluoroquinolones Due to qnrA1 and qnrS1 Genes or a gyrA Mutation on Ciprofloxacin Bactericidal Activity in a Murine Model of Escherichia coli Urinary Tract Infection

2009 ◽  
Vol 53 (10) ◽  
pp. 4292-4297 ◽  
Author(s):  
Nicolas Allou ◽  
Emmanuelle Cambau ◽  
Laurent Massias ◽  
Françoise Chau ◽  
Bruno Fantin
Keyword(s):  
Escherichia Coli ◽  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Bactericidal Activity ◽  
Murine Model ◽  
Bacterial Counts ◽  
Low Level ◽  
The Impact ◽  
Level Resistance

ABSTRACT We investigated the impact of low-level resistance to fluoroquinolones on the bactericidal activity of ciprofloxacin in a murine model of urinary tract infection. The susceptible Escherichia coli strain CFT073 (ciprofloxacin MIC [CIP MIC] of 0.008 μg/ml) was compared to its transconjugants harboring qnrA1 or qnrS1 and to an S83L gyrA mutant. The three derivatives showed similar low-level resistance to fluoroquinolones (CIP MICs, 0.25 to 0.5 μg/ml). Bactericidal activity measured in vitro after 1, 3, and 6 h of exposure to 0.5 μg/ml of ciprofloxacin was significantly lower for the derivative strains (P < 0.01). In the murine model of urinary tract infection (at least 45 mice inoculated per strain), mice were treated with a ciprofloxacin regimen of 2.5 mg/kg, given subcutaneously twice daily for 2 days. In mice infected with the susceptible strain, ciprofloxacin significantly decreased viable bacterial counts (log10 CFU/g of tissue) in the bladder (4.2 ± 0.5 versus 5.5 ± 1.3; P = 0.001) and in the kidney (3.6 ± 0.8 versus 5.0 ± 1.1; P = 0.003) compared with those of untreated mice. In contrast, no significant decrease in viable bacterial counts was observed with any of the three derivative strains. The area under the concentration-time curve from 0 to 24 h/MIC and the maximum concentration of drug in serum/MIC ratios measured in plasma were indeed equal to 827 and 147, respectively, for the parental strain, and only 12.4 to 24.8 and 2.2 to 4.4, respectively, for the derivative strains. In conclusion, low-level resistance to fluoroquinolones conferred by a qnr gene is associated with decreased bactericidal activity of ciprofloxacin, similar to that obtained with a gyrA mutation.

scholarly journals Activity of temocillin in a murine model of urinary tract infection due toEscherichia coliproducing or not producing the ESBL CTX-M-15

2015 ◽  
Vol 70 (5) ◽  
pp. 1466-1472 ◽  
Author(s):  
J. F. Soubirou ◽  
B. Rossi ◽  
C. Couffignal ◽  
E. Ruppé ◽  
F. Chau ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Murine Model

scholarly journals Characterization of theebpfmpilus-encoding operon ofEnterococcus faeciumand its role in biofilm formation and virulence in a murine model of urinary tract infection

Virulence ◽  
2010 ◽  
Vol 1 (4) ◽  
pp. 236-246 ◽  
Author(s):  
Jouko Sillanpää ◽  
Sreedhar R. Nallapareddy ◽  
Kavindra V. Singh ◽  
Vittal P. Prakash ◽  
Timothy Fothergill ◽  
...  
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Tract Infection ◽  
Murine Model ◽  
Biofilm Formation

scholarly journals The Periplasmic Trehalase Affects Type 1 Fimbria Production and Virulence of Extraintestinal PathogenicEscherichia coliStrain MT78

2018 ◽  
Vol 86 (8) ◽  
Author(s):  
Daniel Brisotto Pavanelo ◽  
Sébastien Houle ◽  
Letícia Beatriz Matter ◽  
Charles Martin Dozois ◽  
Fabiana Horn
Keyword(s):  
Urinary Tract Infection ◽  
Urinary Tract ◽  
Carbon Source ◽  
Tract Infection ◽  
Osmotic Stress ◽  
Murine Model ◽  
Content Type ◽  
Type 1 Fimbriae ◽  
Expec Strain

ABSTRACTExtraintestinal pathogenicEscherichia coli(ExPEC) is responsible for various infections outside the gastrointestinal tract in humans and other animals. ExPEC strain MT78 is invasive to various nonphagocytic cells and highly virulentin vivo. To identify genes required for invasion of nonphagocytic cells by this strain, we applied signature-tagged mutagenesis to generate a library of mutants and tested them for invasion of avian fibroblasts. Mutants showing reduced cellular invasion included those with insertions in thefimoperon, encoding type 1 fimbriae. Another attenuated mutant showed a disruption in thetreAgene, which encodes a periplasmic trehalase. The substrate of TreA, trehalose, can be metabolized and used as a carbon source or can serve as an osmoprotectant under conditions of osmotic stress inE. coliK-12. We generated and characterized mutant MT78ΔtreA. In contrast to the wild type, MT78ΔtreAwas able to grow under osmotic stress caused by 0.6 M urea but not in minimal M9 medium with trehalose as the only carbon source. It presented decreased association and invasion of avian fibroblasts, decreased yeast agglutination titer, and impaired type 1 fimbria production. In a murine model of urinary tract infection, MT78ΔtreAwas less able to colonize the bladder. All phenotypes were rescued in the complemented mutant. Our results show that thetreAgene is needed for optimal production of type 1 fimbriae in ExPEC strain MT78 and that loss oftreAsignificantly reduces its cell invasion capacity and colonization of the bladder in a murine model of urinary tract infection.

Sign in / Sign up

Export Citation Format

Share Document