scholarly journals A cohabiting bacterium alters the spectrum of short RNAs secreted byEscherichia coli

2018 ◽  
Vol 365 (24) ◽  
Author(s):  
Olga V Alikina ◽  
Olga A Glazunova ◽  
Alexandr A Bykov ◽  
Sergey S Kiselev ◽  
Maria N Tutukina ◽  
...  
Keyword(s):  
Short Rnas ◽  
Byescherichia Coli

Short RNAs, potential novel molecular markers for higher plants

2012 ◽  
Vol 20 (1) ◽  
pp. 86-93
Author(s):  
Liu Wen ◽  
Liang Hong ◽  
Yang Biren ◽  
Lin Jiewen ◽  
Hu Yanji
Keyword(s):  
Molecular Markers ◽  
Higher Plants ◽  
Short Rnas

Characterization of the inducer of short transcripts, a human immunodeficiency virus type 1 transcriptional element that activates the synthesis of short RNAs

1993 ◽  
Vol 13 (2) ◽  
pp. 1251-1263
Author(s):  
M Sheldon ◽  
R Ratnasabapathy ◽  
N Hernandez
Keyword(s):  
Human Immunodeficiency Virus ◽  
Human Immunodeficiency Virus Type ◽  
Virus Type ◽  
Regulatory Region ◽  
Short Rnas ◽  
Immunodeficiency Virus ◽  
R Region ◽  
Transcription Complexes ◽  
Hiv 1

The inducer of short transcripts, or IST, is an unusual transcriptional element located downstream of the human immunodeficiency virus type 1 (HIV-1) promoter. IST activates HIV-1 transcription, but the resulting RNAs are short and end at approximately position +59. IST, therefore, appears to promote the formation of transcription complexes that are unable to elongate efficiently. This activity contrasts with that of TAR, the target for Tat trans-activation, which upon binding of the viral protein Tat promotes the formation of transcription complexes capable of efficient elongation through the entire viral genome. We have localized and characterized the IST element. Our results indicate that IST is located mainly between positions -5 and +26, although the sequences from positions +40 to +59 also contribute to IST activity. Unlike TAR, which is an RNA element, IST appears to be a DNA element. Thus, the HIV-1 R region is a complex regulatory region with RNA and DNA elements that promote the formation of transcription complexes with different elongation properties.

Tetramer formation of a variant type human transthyretin (prealbumin) produced byEscherichia coli expression system

1989 ◽  
Vol 163 (2) ◽  
pp. 851-859 ◽  
Author(s):  
Hirokazu Furuya ◽  
Masamitsu Nakazato ◽  
Maria Joao Mascarenhas Saraiva ◽  
Pedro P. Costa ◽  
Hiroyuki Sasaki ◽  
...  
Keyword(s):  
Expression System ◽  
Variant Type ◽  
Tetramer Formation ◽  
Byescherichia Coli

scholarly journals Circulating miRNAs as Potential Biomarkers for Celiac Disease Development

2021 ◽  
Vol 12 ◽  
Author(s):  
Ineke L. Tan ◽  
Rodrigo Coutinho de Almeida ◽  
Rutger Modderman ◽  
Anna Stachurska ◽  
Jackie Dekens ◽  
...  
Keyword(s):  
Celiac Disease ◽  
European Population ◽  
Circulating Mirnas ◽  
Serum Samples ◽  
Short Rnas ◽  
Individual Mirnas ◽  
Mirna Sequencing ◽  
Diagnostic Biopsy ◽  
Transcriptional Gene Regulation ◽  
Meta Analyses

Background & AimsCeliac disease (CeD), an immune-mediated disease with enteropathy triggered by gluten, affects ~1% of the general European population. Currently, there are no biomarkers to predict CeD development. MicroRNAs (miRNAs) are short RNAs involved in post-transcriptional gene regulation, and certain disease- and stage-specific miRNA profiles have been found previously. We aimed to investigate whether circulating miRNAs can predict the development of CeD.MethodsUsing next-generation miRNA-sequencing, we determined miRNAs in >200 serum samples from 53 participants of the PreventCD study, of whom 33 developed CeD during follow-up. Following study inclusion at 3 months of age, samples were drawn at predefined ages, diagnosis (first anti-transglutaminase antibody (TGA) positivity or diagnostic biopsy) and after the start of a gluten-free diet (GFD). This allowed identification of circulating miRNAs that are deregulated before TGA positivity. For validation of the biomarkers for CeD and GFD response, two additional cohorts were included in subsequent meta-analyses. Additionally, miRNAs were measured in duodenal biopsies in a case-control cohort.Results53 circulating miRNAs were increased (27) or decreased (26) in CeD versus controls. We assessed specific trends in these individual miRNAs in the PreventCD cohort by grouping the pre-diagnostic samples of the CeD patients (all had negative TGA) by how close to seroconversion (first sample positive TGA) the samples were taken. 8/53 miRNAs differed significantly between controls and samples taken <1 year before TGA positivity: miR-21-3p, miR-374a-5p, 144-3p, miR-500a-3p, miR-486-3p let-7d-3p, let-7e-5p and miR-3605-3p. 6/26 downregulated miRNAs reconstituted upon GFD, including miR-150-5p/-3p, whereas no upregulated miRNAs were downregulated upon GFD. 15/53 biomarker candidates also differed between CeD biopsies and controls, with a concordant direction, indicating that these circulating miRNAs might originate from the intestine.ConclusionsWe identified 53 circulating miRNAs that are potential early biomarkers for CeD, of which several can be detected more than a year before TGA positivity and some start to normalize upon GFD.

scholarly journals Global Analysis of Short RNAs Reveals Widespread Promoter-Proximal Stalling and Arrest of Pol II in Drosophila

Science ◽  
2009 ◽  
Vol 327 (5963) ◽  
pp. 335-338 ◽  
Author(s):  
S. Nechaev ◽  
D. C. Fargo ◽  
G. dos Santos ◽  
L. Liu ◽  
Y. Gao ◽  
...  
Keyword(s):  
Global Analysis ◽  
Pol Ii ◽  
Short Rnas
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