scholarly journals Group II intron splicing factors derived by diversification of an ancient RNA-binding domain

2003 ◽  
Vol 22 (15) ◽  
pp. 3919-3929 ◽  
Author(s):  
G. J. Ostheimer
Keyword(s):  
Rna Binding ◽  
Group Ii Intron ◽  
Binding Domain ◽  
Splicing Factors ◽  
Intron Splicing ◽  
Rna Binding Domain ◽  
Group Ii

scholarly journals A plant-specific RNA-binding domain revealed through analysis of chloroplast group II intron splicing

2009 ◽  
Vol 106 (11) ◽  
pp. 4537-4542 ◽  
Author(s):  
T. S. Kroeger ◽  
K. P. Watkins ◽  
G. Friso ◽  
K. J. van Wijk ◽  
A. Barkan
Keyword(s):  
Rna Binding ◽  
Group Ii Intron ◽  
Binding Domain ◽  
Intron Splicing ◽  
Rna Binding Domain ◽  
Group Ii

scholarly journals Group II intron splicing factors in plant mitochondria

2014 ◽  
Vol 5 ◽  
Author(s):  
Gregory G. Brown ◽  
Catherine Colas des Francs-Small ◽  
Oren Ostersetzer-Biran
Keyword(s):  
Plant Mitochondria ◽  
Group Ii Intron ◽  
Splicing Factors ◽  
Intron Splicing ◽  
Group Ii

scholarly journals Nuclear Encoded RNA Splicing Factors in Plant Mitochondria

2009 ◽  
Author(s):  
Oren Ostersetzer-Biran ◽  
Alice Barkan
Keyword(s):  
Rna Splicing ◽  
Plant Mitochondria ◽  
Group Ii Intron ◽  
Nuclear Genes ◽  
Metabolic Energy ◽  
Splicing Factors ◽  
Group Ii Introns ◽  
Intron Splicing ◽  
Group Ii ◽  
Mitochondrial Introns

Mitochondria are the site of respiration and numerous other metabolic processes required for plant growth and development. Increased demands for metabolic energy are observed during different stages in the plants life cycle, but are particularly ample during germination and reproductive organ development. These activities are dependent upon the tight regulation of the expression and accumulation of various organellar proteins. Plant mitochondria contain their own genomes (mtDNA), which encode for a small number of genes required in organellar genome expression and respiration. Yet, the vast majority of the organellar proteins are encoded by nuclear genes, thus necessitating complex mechanisms to coordinate the expression and accumulation of proteins encoded by the two remote genomes. Many organellar genes are interrupted by intervening sequences (introns), which are removed from the primary presequences via splicing. According to conserved features of their sequences these introns are all classified as “group-II”. Their splicing is necessary for organellar activity and is dependent upon nuclear-encoded RNA-binding cofactors. However, to-date, only a tiny fraction of the proteins expected to be involved in these activities have been identified. Accordingly, this project aimed to identify nuclear-encoded proteins required for mitochondrial RNA splicing in plants, and to analyze their specific roles in the splicing of group-II intron RNAs. In non-plant systems, group-II intron splicing is mediated by proteins encoded within the introns themselves, known as maturases, which act specifically in the splicing of the introns in which they are encoded. Only one mitochondrial intron in plants has retained its maturaseORF (matR), but its roles in organellar intron splicing are unknown. Clues to other proteins required for organellar intron splicing are scarce, but these are likely encoded in the nucleus as there are no other obvious candidates among the remaining ORFs within the mtDNA. Through genetic screens in maize, the Barkan lab identified numerous nuclear genes that are required for the splicing of many of the introns within the plastid genome. Several of these genes are related to one another (i.e. crs1, caf1, caf2, and cfm2) in that they share a previously uncharacterized domain of archaeal origin, the CRM domain. The Arabidopsis genome contains 16 CRM-related genes, which contain between one and four repeats of the domain. Several of these are predicted to the mitochondria and are thus postulated to act in the splicing of group-II introns in the organelle(s) to which they are localized. In addition, plant genomes also harbor several genes that are closely related to group-II intron-encoded maturases (nMats), which exist in the nucleus as 'self-standing' ORFs, out of the context of their cognate "host" group-II introns and are predicted to reside within the mitochondria. The similarity with known group-II intron splicing factors identified in other systems and their predicted localization to mitochondria in plants suggest that nuclear-encoded CRM and nMat related proteins may function in the splicing of mitochondrial-encoded introns. In this proposal we proposed to (i) establish the intracellular locations of several CRM and nMat proteins; (ii) to test whether mutations in their genes impairs the splicing of mitochondrial introns; and to (iii) determine whether these proteins are bound to the mitochondrial introns in vivo.  

scholarly journals APO1 Promotes the Splicing of Chloroplast Group II Introns and Harbors a Plant-Specific Zinc-Dependent RNA Binding Domain

2011 ◽  
Vol 23 (3) ◽  
pp. 1082-1092 ◽  
Author(s):  
Kenneth P. Watkins ◽  
Margarita Rojas ◽  
Giulia Friso ◽  
Klaas J. van Wijk ◽  
Jörg Meurer ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Domain ◽  
Group Ii Introns ◽  
Rna Binding Domain ◽  
Group Ii

scholarly journals Requirement for C-terminal Extension to the RNA Binding Domain for Efficient RNA Binding by Ribosomal Protein L2

2002 ◽  
Vol 66 (3) ◽  
pp. 682-684 ◽  
Author(s):  
Takeshi HAYASHI ◽  
Maino TAHARA ◽  
Kenta IWASAKI ◽  
Yoshiaki KOUZUMA ◽  
Makoto KIMURA
Keyword(s):  
Ribosomal Protein ◽  
Rna Binding ◽  
Binding Domain ◽  
Ribosomal Protein L2 ◽  
Rna Binding Domain

K160 in the RNA‐binding domain of the orf virus virulence factor OV20.0 is critical for its functions in counteracting host antiviral defense

FEBS Letters ◽  
2021 ◽  
Author(s):  
Guan‐Ru Liao ◽  
Yeu‐Yang Tseng ◽  
Ching‐Yu Tseng ◽  
Ying‐Ping Huang ◽  
Ching‐Hsiu Tsai ◽  
...  
Keyword(s):  
Virulence Factor ◽  
Rna Binding ◽  
Binding Domain ◽  
Orf Virus ◽  
Antiviral Defense ◽  
Rna Binding Domain
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