scholarly journals DNA base excision repair of uracil residues in reconstituted nucleosome core particles

2002 ◽  
Vol 21 (21) ◽  
pp. 5943-5952 ◽  
Author(s):  
H. Nilsen
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Nucleosome Core ◽  
Dna Base Excision Repair ◽  
Nucleosome Core Particles ◽  
Dna Base ◽  
Base Excision ◽  
Core Particles

scholarly journals Histones participate in base excision repair of 8-oxodGuo by transiently cross-linking with active repair intermediates in nucleosome core particles

2020 ◽  
Author(s):  
Mengtian Ren ◽  
Mengdi Shang ◽  
Huawei Wang ◽  
Zhen Xi ◽  
Chuanzheng Zhou
Keyword(s):  
Base Excision Repair ◽  
Oxidative Dna Damage ◽  
Excision Repair ◽  
Cross Linking ◽  
Nucleosome Core ◽  
Nucleosome Core Particles ◽  
Lyase Activity ◽  
Base Excision ◽  
Ap Lyase ◽  
Core Particles

Abstract 8-Oxo-7,8-dihydro-2′-deoxyguanosine (8-oxodGuo) is a biomarker of oxidative DNA damage and can be repaired by hOGG1 and APE1 via the base excision repair (BER) pathway. In this work, we studied coordinated BER of 8-oxodGuo by hOGG1 and APE1 in nucleosome core particles and found that histones transiently formed DNA-protein cross-links (DPCs) with active repair intermediates such as 3′-phospho-α,β-unsaturated aldehyde (PUA) and 5′-deoxyribosephosphate (dRP). The effects of histone participation could be beneficial or deleterious to the BER process, depending on the circumstances. In the absence of APE1, histones enhanced the AP lyase activity of hOGG1 by cross-linking with 3′-PUA. However, the formed histone-PUA DPCs hampered the subsequent repair process. In the presence of APE1, both the AP lyase activity of hOGG1 and the formation of histone-PUA DPCs were suppressed. In this case, histones could catalyse removal of the 5′-dRP by transiently cross-linking with the active intermediate. That is, histones promoted the repair by acting as 5′-dRP lyases. Our findings demonstrate that histones participate in multiple steps of 8-oxodGuo repair in nucleosome core particles, highlighting the diverse roles that histones may play during DNA repair in eukaryotic cells.

DNA base excision repair and nucleotide excision repair proteins in malignant salivary gland tumors

2021 ◽  
Vol 121 ◽  
pp. 104987
Author(s):  
Fernanda Aragão Felix ◽  
Leorik Pereira da Silva ◽  
Maria Luiza Diniz de Sousa Lopes ◽  
Ana Paula Veras Sobral ◽  
Roseana de Almeida Freitas ◽  
...  
Keyword(s):  
Salivary Gland ◽  
Nucleotide Excision Repair ◽  
Base Excision Repair ◽  
Excision Repair ◽  
Salivary Gland Tumors ◽  
Dna Base Excision Repair ◽  
Dna Base ◽  
Nucleotide Excision ◽  
Base Excision ◽  
Malignant Salivary Gland Tumors

A Versatile DNA Nanochip for Direct Analysis of DNA Base-Excision Repair

2010 ◽  
Vol 49 (49) ◽  
pp. 9412-9416 ◽  
Author(s):  
Masayuki Endo ◽  
Yousuke Katsuda ◽  
Kumi Hidaka ◽  
Hiroshi Sugiyama
Keyword(s):  
Base Excision Repair ◽  
Excision Repair ◽  
Direct Analysis ◽  
Dna Base Excision Repair ◽  
Dna Base ◽  
Base Excision
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