Relating Aerial Deposition of Entomophaga maimaiga Conidia (Zoopagomycota: Entomophthorales) to Mortality of Gypsy Moth (Lepidoptera: Erebidae) Larvae and Nearby Defoliation

2019 ◽  
Vol 48 (5) ◽  
pp. 1214-1222 ◽  
Author(s):  
Joseph S Elkinton ◽  
Tonya D Bittner ◽  
Valerie J Pasquarella ◽  
George H Boettner ◽  
Andrew M Liebhold ◽  
...  
Keyword(s):  
New England ◽  
Gypsy Moth ◽  
Landsat Imagery ◽  
The United States ◽  
Viral Pathogen ◽  
Entomophaga Maimaiga ◽  
Gypsy Moths ◽  
Airborne Conidia ◽  
Late Instar ◽  
Highly Correlated

Abstract We collected data on mortality of late-instar gypsy moth, Lymantria dispar (L.), from outbreak populations over 4 wk in June 2017 at 10 sites in the New England region of the United States, along with estimated rainfall at these sites. Deposition of airborne conidia of the fungal pathogen, Entomophaga maimaiga Humber, Shimazu & R.S. Soper, was measured at these same sites as well as at seven other locations in New England. We also quantified the geographical distribution of gypsy moth-caused defoliation in New England in 2017 and 2018 from Landsat imagery. Weekly mortality of gypsy moth larvae caused by E. maimaiga correlated with local deposition of conidia from the previous week, but not with rainfall. Mortality from this pathogen reached a peak during the last 2 wk of gypsy moth larval development and always exceeded that caused by LdNPV, the viral pathogen of gypsy moth that has long been associated with gypsy moth outbreaks, especially prior to 1989. Cotesia melanoscela (Ratzeburg) was by far the most abundant parasitoid recovered and caused an average of 12.6% cumulative parasitism, but varied widely among sites. Deposition of E. maimaiga conidia was highly correlated with percent land area defoliated by gypsy moths within distances of 1 and 2 km but was not significantly correlated with defoliation at distances greater than 2 km. This is the first study to relate deposition of airborne conidia of E. maimaiga to mortality of gypsy moths from that agent.

scholarly journals Modification of a Pollen Trap Design To Capture Airborne Conidia of Entomophaga maimaiga and Detection of Conidia by Quantitative PCR

2017 ◽  
Vol 83 (17) ◽  
Author(s):  
Tonya D. Bittner ◽  
Ann E. Hajek ◽  
Andrew M. Liebhold ◽  
Harold Thistle
Keyword(s):  
Quantitative Pcr ◽  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Fungal Pathogen ◽  
Large Scale ◽  
The United States ◽  
Trap Design ◽  
Content Type ◽  
Entomophaga Maimaiga ◽  
Airborne Conidia

ABSTRACT The goal of this study was to develop effective and practical field sampling methods for quantification of aerial deposition of airborne conidia of Entomophaga maimaiga over space and time. This important fungal pathogen is a major cause of larval death in invasive gypsy moth (Lymantria dispar) populations in the United States. Airborne conidia of this pathogen are relatively large (similar in size to pollen), with unusual characteristics, and require specialized methods for collection and quantification. Initially, dry sampling (settling of spores from the air onto a dry surface) was used to confirm the detectability of E. maimaiga at field sites with L. dispar deaths caused by E. maimaiga, using quantitative PCR (qPCR) methods. We then measured the signal degradation of conidial DNA on dry surfaces under field conditions, ultimately rejecting dry sampling as a reliable method due to rapid DNA degradation. We modified a chamber-style trap commonly used in palynology to capture settling spores in buffer. We tested this wet-trapping method in a large-scale (137-km) spore-trapping survey across gypsy moth outbreak regions in Pennsylvania undergoing epizootics, in the summer of 2016. Using 4-day collection periods during the period of late instar and pupal development, we detected variable amounts of target DNA settling from the air. The amounts declined over the season and with distance from the nearest defoliated area, indicating airborne spore dispersal from outbreak areas. IMPORTANCE We report on a method for trapping and quantifying airborne spores of Entomophaga maimaiga, an important fungal pathogen affecting gypsy moth (Lymantria dispar) populations. This method can be used to track dispersal of E. maimaiga from epizootic areas and ultimately to provide critical understanding of the spatial dynamics of gypsy moth-pathogen interactions.

Comparison of Aerially-Applied Gypchek Strains Against Gypsy Moth (Lepidoptera: Lymantriidae) in the Presence of an Entomophaga maimaiga Epizootic

2005 ◽  
Vol 40 (4) ◽  
pp. 446-460 ◽  
Author(s):  
R. E. Webb ◽  
G. B. White ◽  
J. D. Podgwaite ◽  
V. D'Amico ◽  
J. Slavicek ◽  
...  
Keyword(s):  
Gypsy Moth ◽  
Larval Mortality ◽  
Larval Population ◽  
Usda Forest Service ◽  
Entomophaga Maimaiga ◽  
Wave Effects ◽  
Late Instar ◽  
Fungal Entomopathogen ◽  
Occlusion Bodies ◽  
Second Wave

The standard strain (LDP-226) of Gypchek®, a nucleopolyhedrovirus product registered by the USDA Forest Service against the gypsy moth, Lymantria dispar (L.), was compared against a strain, LdMNPV-203NL (NL = nonliquefying), that was developed for production in cell culture. Both strains were applied by air to U.S. government property in Prince Georges Co., MD, in early May 2003 at the rate of 1 × 1012 occlusion bodies per ha. The two goals of the study were (1) to compare the first and second wave effects of the two strains against gypsy moth populations; and (2) to delineate the combined effects of the applied virus and the expected epizootic of the gypsy moth specialist fungal entomopathogen Entomophaga maimaiga Humber, Shimazu, and Soper. Heavy rainfall in May and June preceded a massive epizootic of E. maimaiga, whose effects did not mask the first wave of viral mortality. When the effect of application sequence was considered, it was concluded that the two strains were equivalent in their first-wave impacts. High fungal-induced mid and late-season gypsy moth larval mortality suppressed the second wave of virus at all evaluation sites. There were no obvious differences in the second waves engendered by the two LdNPV strains in the greatly reduced late-instar larval population.

Formation and germination of Entomophaga maimaiga azygospores

1997 ◽  
Vol 75 (10) ◽  
pp. 1739-1747 ◽  
Author(s):  
Ann E. Hajek ◽  
Richard A. Humber
Keyword(s):  
Gypsy Moth ◽  
Lymantria Dispar ◽  
Early Summer ◽  
Resting Spore ◽  
Body Development ◽  
Entomophaga Maimaiga ◽  
Resting Spores ◽  
Late Instar ◽  
Hyphal Bodies ◽  
Hyphal Body

Azygospores (resting spores) of the gypsy moth fungal pathogen Entomophaga maimaiga are produced in abundance during late spring and early summer in late-instar gypsy moth larvae (Lymantria dispar). Azygospores subsequently form, each from an individual hyphal body. Development of azygospores occurs asynchronously over several days; by 5 days after host death, greater than 60% of fungal cells had matured from hyphal bodies to the final double-walled resting state. Azygospores undergo constitutive dormancy and, under field conditions, will not germinate for approximately 9 months after production. Azygospores do not require nutrients to germinate. Germination of field-collected resting spores under laboratory conditions began more than 2 days after transfer from the field to the laboratory. Higher levels of germination occurred with a 14 h L: 10 h D cycle compared with 13 h L: 11 h D or 12 h L: 12 h D. Azygospores germinate relatively slowly and germination rates were greatest between 4 and 8 days, with a total of 71.8 or 72.5% germination by 16 days at 14 h L: 10 h D and 15 or 20 °C, respectively. During 1994 and 1995, resting spores began causing infections in experimental larvae in early May, about 1 – 2 weeks prior to gypsy moth egg hatch, and ceased causing infections in mid to late June, when late instars were present. This latter timing is a correction of previously reported information. Bioassays investigating resting spore activity determined that during 1994, once resting spores began germinating in the field, levels of infection were positively associated with soil moisture. Key words: azygospores, resting spores, entomopathogenic fungi, Entomophaga maimaiga, Lymantria dispar, biological control.

THE NATURE AND ENVIRONMENT OF COST MANAGEMENT AMONG EARLY NINETEENTH CENTURY U.S. TEXITLE MANUFACTURERS

1992 ◽  
Vol 19 (2) ◽  
pp. 1-24 ◽  
Author(s):  
Thomas Tyson
Keyword(s):  
Nineteenth Century ◽  
New England ◽  
Management Practices ◽  
Cost Management ◽  
Cost Accounting ◽  
The United States ◽  
Social Forces ◽  
Cost Information ◽  
Early Nineteenth Century ◽  
Textile Mills

Several authors have suggested that a particular managerial component was needed before cost accounting could be fully used for accountability and disciplinary purposes. They argue that the marriage of managerialism and accounting first occurred in the United States at the Springfield Armory after 1840. They generally downplay the quality and usefulness of cost accounting at the New England textile mills before that time and call for a re-examination of original mill records from a disciplinary perspective. This paper reports the results of such a re-examination. It initially describes the social and economic environment of U.S. textile manufacturing in New England in the early nineteenth century. Selected cost memos and reports are described and analyzed to indicate the nature and scope of costing undertaken at the mills in Lowell, Massachusetts, in the late 1820s and early 1830s. The paper discusses how particular cost information was used and speculates why certain more modern procedures were not adopted. Its major finding is that cost management practices fully measured up to the business complexities, economic pressures, and social forces of the day.

scholarly journals Exposure of Domestic Cats to Three Zoonotic Bartonella Species in the United States

Pathogens ◽  
2021 ◽  
Vol 10 (3) ◽  
pp. 354
Author(s):  
Lynn M. Osikowicz ◽  
Kalanthe Horiuchi ◽  
Irina Goodrich ◽  
Edward B. Breitschwerdt ◽  
Bruno Chomel ◽  
...  
Keyword(s):  
New England ◽  
The United States ◽  
Severe Illness ◽  
Domestic Cats ◽  
Bartonella Species ◽  
Serum Samples ◽  
North Central ◽  
Middle Atlantic ◽  
South Central ◽  
Central Regions

Cat-associated Bartonella species, which include B. henselae, B. koehlerae, and B. clarridgeiae, can cause mild to severe illness in humans. In the present study, we evaluated 1362 serum samples obtained from domestic cats across the U.S. for seroreactivity against three species and two strain types of Bartonella associated with cats (B. henselae type 1, B. henselae type 2, B. koehlerae, and B. clarridgeiae) using an indirect immunofluorescent assay (IFA). Overall, the seroprevalence at the cutoff titer level of ≥1:64 was 23.1%. Seroreactivity was 11.1% and 3.7% at the titer level cutoff of ≥1:128 and at the cutoff of ≥1:256, respectively. The highest observation of seroreactivity occurred in the East South-Central, South Atlantic, West North-Central, and West South-Central regions. The lowest seroreactivity was detected in the East North-Central, Middle Atlantic, Mountain, New England, and Pacific regions. We observed reactivity against all four Bartonella spp. antigens in samples from eight out of the nine U.S. geographic regions.

Expansion of Groundnut ringspot virus Host and Geographic Ranges in Solanaceous Vegetables in Peninsular Florida

2011 ◽  
Vol 12 (1) ◽  
pp. 34 ◽  
Author(s):  
Craig G. Webster ◽  
William W. Turechek ◽  
H. Charles Mellinger ◽  
Galen Frantz ◽  
Nancy Roe ◽  
...  
Keyword(s):  
United States ◽  
The United States ◽  
Vegetable Production ◽  
Viral Pathogen ◽  
Geographic Ranges ◽  
First Report ◽  
Geographic Spread ◽  
Wide Range ◽  
Production Areas ◽  
Solanaceous Plants

To the best of our knowledge, this is the first report of GRSV infecting tomatillo and eggplant, and it is the first report of GRSV infecting pepper in the United States. This first identification of GRSV-infected crop plants in commercial fields in Palm Beach and Manatee Counties demonstrates the continuing geographic spread of the virus into additional vegetable production areas of Florida. This information indicates that a wide range of solanaceous plants is likely to be infected by this emerging viral pathogen in Florida and beyond. Accepted for publication 27 June 2011. Published 25 July 2011.

COI gene geographic variation of Gypsy moth (Lepidoptera: Lymantriidae) and a TaqMan PCR diagnostic assay

DNA Barcodes ◽  
2014 ◽  
Vol 2 (1) ◽  
Author(s):  
Lu Qian ◽  
Yulin An ◽  
Junxian Song ◽  
Mei Xu ◽  
Jianlin Ye ◽  
...  
Keyword(s):  
Amino Acid ◽  
Gypsy Moth ◽  
Diagnostic Tool ◽  
Genetic Difference ◽  
Science And Technology ◽  
The United States ◽  
Coi Gene ◽  
Taqman Assay ◽  
Base Substitution ◽  
Separate Branch

AbstractGypsy moth, an important forest/urban pest worldwide, is separated into the European and Asian subspecies, and has important quarantine significance. Diagnostic technique that can accurately and quickly distinguish subspecies is lacking. This study aimed to evaluate genetic difference between the subspecies, and subsequently to develop a reliable and high throughput molecular based diagnostic tool for distinguishing the subspecies. COI genes of 25 gypsy moth samples from China, Russia, Mongolia, Japan and the United States were sequenced. DNASTAR analysis revealed that gypsy moth COI gene was 1531bp long. The UPGM phylogenetic tree constructed based on the COI gene indicated that European subspecies (U.S. population) and Asian subspecies were distinctively divided into two branches. Japanese populations had a far distantly relationship with other Asian populations forming a separate branch. There was a single base substitution (base transformation only) at 14 consistent locations between Asian and American populations, but 13 of them coded the same amino acid. A MGB proper and TaqMan assay was designed base on the base substitution at 406th bp that coded a different amino acid. This allele typing assay took only 4 hours and could accurately distinguish gypsy moth subspecies of Europe and Asia. The study enriches the knowledge basis of genetic differentiation of gypsy moth subspecies. And more importantly the TaqMan assay is the first report of such diagnostic tool that could deliver rapid and accurate results and suitable for routine quarantine inspections to distinguish Asian and European gypsy moth subspecies. This study was supported by the Ministry of Science and Technology of the People’s Republic of China (Science and technology supporting project: 2012BAK11B03; International cooperation project: 2009DFA31950) and Jiangsu Entry and Exit Inspection and Quarantine Bureau (2014KJ45).

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