Inhibition ofHelicoverpa zea(Lepidoptera: Noctuidae) Growth by Transgenic Corn Expressing Bt Toxins and Development of Resistance to Cry1Ab

2015 ◽  
Vol 44 (4) ◽  
pp. 1275-1285 ◽  
Author(s):  
Dominic D. Reisig ◽  
Francis P. F. Reay-Jones
Keyword(s):  
Transgenic Corn ◽  
Development Of Resistance ◽  
Bt Toxins ◽  
Lepidoptera Noctuidae

scholarly journals Differences between two populations of bollworm, Helicoverpa zea (Lepidoptera: Noctuidae), with variable measurements of laboratory susceptibilities to Bt toxins exposed to non-Bt and Bt cottons in large field cages

PLoS ONE ◽  
2019 ◽  
Vol 14 (3) ◽  
pp. e0212567 ◽  
Author(s):  
Nathan S. Little ◽  
Blake H. Elkins ◽  
R. Michelle Mullen ◽  
Omaththage P. Perera ◽  
Katherine A. Parys ◽  
...  
Keyword(s):  
Helicoverpa Zea ◽  
Large Field ◽  
Bt Toxins ◽  
Lepidoptera Noctuidae ◽  
Two Populations

Positive association between resistance to Bacillus thuringiensis and overwintering survival of cabbage loopers, Trichoplusia ni (Lepidoptera: Noctuidae)

2008 ◽  
Vol 98 (3) ◽  
pp. 317-322 ◽  
Author(s):  
V. Caron ◽  
J.H. Myers
Keyword(s):  
Bacillus Thuringiensis ◽  
Trichoplusia Ni ◽  
Positive Association ◽  
Pest Species ◽  
Development Of Resistance ◽  
Trade Offs ◽  
Major Pest ◽  
Selection For ◽  
Overwintering Survival ◽  
Lepidoptera Noctuidae

AbstractDevelopment of resistance to insecticides has generally been associated with fitness costs that may be magnified under challenging conditions. Lepidopterans which are resistant to the biopesticide Bacillus thuringiensis subsp. kurstaki (Btk) have been shown to have reduced fitness, such as lower survival when subjected to overwintering stress. Recently, resistance to Btk has been found in some populations of Trichoplusia ni Hübner in greenhouses in British Columbia. This situation provides an opportunity to investigate potential trade-offs between overwintering survival and insecticide resistance in a major pest species. Here, we assess the survival and eventual fecundity of Btk resistant and susceptible T. ni pupae exposed to cool temperatures. Contrary to our expectations, resistant T. ni had higher overwintering survival than susceptible individuals. This is the first account of a potential advantage associated with Btk resistance. Resistant and susceptible moths had reduced fecundity and smaller progeny after cold exposure compared to controls, and this may counteract the survival advantage. Nevertheless, it seems unlikely that this is sufficient to select out the resistant phenotype in the presence of strong selection for resistance and in the absence of immigration of susceptible moths. The appearance of resistance without evidence of a trade-off in overwintering survival presents a major challenge to management in production greenhouses.

Impact of Corn Earworm Injury on Yield of Transgenic Corn Producing Bt Toxins in the Carolinas

2014 ◽  
Vol 107 (3) ◽  
pp. 1101-1109 ◽  
Author(s):  
Francis P. F. Reay-Jones ◽  
Dominic D. Reisig
Keyword(s):  
Corn Earworm ◽  
Transgenic Corn ◽  
Bt Toxins

Field Evaluation of Transgenic Corn Containing a Bacillus thuringiensis Berliner Insecticidal Protein Gene Against Helicoverpa zea (Lepidoptera: Noctuidae)

1996 ◽  
Vol 31 (3) ◽  
pp. 340-346 ◽  
Author(s):  
Steven R. Sims ◽  
Jay C. Pershing ◽  
Barbara J. Reich
Keyword(s):  
Bacillus Thuringiensis ◽  
Helicoverpa Zea ◽  
Negative Relationship ◽  
Field Evaluation ◽  
Corn Earworm ◽  
Insecticidal Protein ◽  
Transgenic Corn ◽  
First Instar ◽  
Transgenic Lines ◽  
Lepidoptera Noctuidae

Twelve independently transformed lines of transgenic corn (Zea mays L.) expressing the CryIA(b) insecticidal protein from Bacillus thuringiensis var. kurstaki were field tested to evaluate their resistance to the corn earworm, Helicoverpa zea (Boddie). Silks of the primary (=top) ears of transgenic [CryIA(b) positive] and isoline control plants [no CryIA(b) protein] were artificially infested with first-instar H. zea larvae and the length of ear penetration was measured after 19 d. Eight of the 12 lines had significantly less ear damage than their respective isoline controls; 3 transgenic lines reduced H. zea feeding damage by > 75% and stunted surviving H. zea larvae. Concentration of the CryIA(b) protein (μg/g fresh weight) in silks of the transgenic lines, determined using ELISA, ranged from 0.0 to 1.28 μg/g. Within transgenic lines, there was a weak (P < 0.06) negative relationship between the concentration of CryIA(b) protein in fresh silks and the length of H. zea ear penetration.

scholarly journals Synergic Interaction between Permethrin and Bt toxins discovered using RNA-seq

2019 ◽  
Author(s):  
Takuma Sakamoto ◽  
Toshinori Kozaki ◽  
Norichika Ogata
Keyword(s):  
Differentially Expressed Gene ◽  
Model Organisms ◽  
Cross Resistance ◽  
Agricultural Pests ◽  
Bt Toxin ◽  
Development Of Resistance ◽  
Bt Toxins ◽  
Increased Susceptibility

AbstractActing against the development of resistance to antibiotics and insecticides, involving negatively correlated cross-resistance (NCR) is an alternative to use- and-discard approach. It is termed NCR that toxic chemicals interact with each other and resistance of target organisms to one chemical is sometimes associated with increased susceptibility to a second chemical when; an allele confers resistance to one toxic chemical and hyper-susceptibility to another, NCR occurs. However, only 11 toxin pairs have been revealed to cause NCR in insects. Finding novel NCRs is needed for integrated pest management. We analyzed permethrin, an insecticide, induced transcriptomes of cultured fat bodies of the silkworm Bombyx mori, a lepidopteran model insect. Differentially expressed gene analyses suggested Bacillus thuringiensis (Bt) toxin was an NCR toxin of permethrin. NCR to permethrin and Bt toxins in Thysanoplusia intermixta, the agricultural pest moth, was examined; the children of permethrin survivor T. intermixta had increased susceptibility to Bt toxin. A novel NCR toxin pair, permethrin and Bt toxin, was discovered. The screening and developmental method for negatively correlated cross-resistance toxins established in this study was effective, in vitro screening using model organisms and in vivo verification using agricultural pests.

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