Extraction, Polymerase Chain Reaction, and Sequencing of a 440 Base Pair Region of the Mitochondrial Cytochrome Oxidase I Gene from Two Species of Acetone-Preserved Damselflies (Odonata: Coenagrionidae, Agrionidae)

1999 ◽  
Vol 28 (2) ◽  
pp. 143-147 ◽  
Author(s):  
Jessica A. Logan
Keyword(s):  
Polymerase Chain Reaction ◽  
Cytochrome Oxidase ◽  
Base Pair ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Chain Reaction ◽  
Cytochrome Oxidase I Gene ◽  
Polymerase Chain ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene

Molecular analysis of mitochondrial cytochrome oxidase I gene of Aedes aegypti L. mosquitoes

2020 ◽  
Vol 23 (1) ◽  
pp. 51-59 ◽  
Author(s):  
Ratnapal Gandhi ◽  
Kamlesh K. Yadav ◽  
Prabhakargouda B. Patil ◽  
Pankaj Bihani ◽  
Bharat Char ◽  
...  
Keyword(s):  
Aedes Aegypti ◽  
Cytochrome Oxidase ◽  
Molecular Analysis ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene

The Comparative Molecular Study between Bombycidae and Saturniidae Based on mtDNA RFLP and Cytochrome Oxidase I Gene Sequences: Implication for Molecular Evolution

1999 ◽  
Vol 54 (7-8) ◽  
pp. 587-594 ◽  
Author(s):  
Jae-Sam Hwang ◽  
Jin-Sung Lee ◽  
Tae-Won Goo ◽  
Hyun-Ah Kang ◽  
Hae-Ryong Sohn ◽  
...  
Keyword(s):  
Cytochrome Oxidase ◽  
Molecular Study ◽  
Cytochrome Oxidase I ◽  
Mitochondrial Cytochrome ◽  
Confidence Limits ◽  
Sequence Identity ◽  
Cytochrome Oxidase I Gene ◽  
Mitochondrial Cytochrome Oxidase ◽  
I Gene ◽  
Mtdna Rflp

Abstract Bombycidae, Saturniidae, mtDNA RFLP, Cytochrome Oxidase I Gene The phylogenetic relationships between Bombyx mori and Bombyx mandarina species of Bombycidae, and Antheraea yamamai and Antheraea pernyi species of Saturniidae were investigated based on mtDNA RFLP and cytochrome oxidase I gene. The sizes of the mtDNA of all the species were estimated at approximately 16 kbp ± 500 bp by total length of all the restricted fragments and no variation in size was recognized. Of the fourteen different restriction endonucleases used, BamHl, Hindlll, Pstl, EcoRl and Xbal showed RFLP. Among these, only Hindlll showed RFLP between B. mori and B. mandarina. A comparative analysis of sequences was also conducted with the mitochondrial cytochrome oxidase I genes of each species. The results indicated that B. mori shared a 97% , 85% and 87% sequence identity with B. mandarina, A. yamamai and A. pernyi, respectively. B. mandarina shared a 87% and 88% sequence identity with A. yamamai and A. penyi, respectively. A. yamamai shared 92% sequence identity with A. pernyi. The results of the phylogenetic analysis exhib­ited monophyly and confidence limits of more than 99% in all trees for both Bombycidae and Saturniidae.

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