Survival and Growth of Bollworm and Tobacco Budworm on Nontransgenic and Transgenic Cotton Expressing a CryIA Insecticidal Protein (Lepidoptera: Noctuidae)

1996 ◽  
Vol 25 (2) ◽  
pp. 250-255 ◽  
Author(s):  
J. L. Halcomb ◽  
J. H. Benedict ◽  
B. Cook ◽  
D. R. Ring
Keyword(s):  
Transgenic Cotton ◽  
Tobacco Budworm ◽  
Insecticidal Protein ◽  
Survival And Growth ◽  
Lepidoptera Noctuidae

The transfer of Bt insecticidal protein to higher tropic levels via a transgenic cotton, then beet armyworm (Lepidoptera: Noctuidae) and their natural enemies

2013 ◽  
Vol 146 (1) ◽  
pp. 1-7 ◽  
Author(s):  
Chun-Xia Chen ◽  
Erzhong Wu ◽  
Yi-Zhong Yang ◽  
Hong-Hua Su
Keyword(s):  
Natural Enemies ◽  
Food Chain ◽  
Transgenic Cotton ◽  
The Body ◽  
Beet Armyworm ◽  
Insecticidal Protein ◽  
Enzyme Linked Immunosorbent Assay ◽  
Bt Cotton ◽  
Bt Toxin ◽  
Lepidoptera Noctuidae

AbstractIn order to determine the transference of Bacillus thuringiensis Berliner (Bacillaceae) (Bt) insecticidal protein in the food chain, enzyme-linked immunosorbent assay was used to detect Bt insecticidal protein levels in transgenic Bt cotton (GK12, New variety 33B and SGK321), Spodoptera exigua (Hübner) (Lepidoptera: Noctuidae) fed on the Bt cotton varieties, and two natural enemies of S. exigua, Chrysoperla carnea (Stephens) (Neuroptera: Chrysopidae) and Microplitis pallidipes Szépligeti (Hymenoptera: Braconidae). The results showed that Bt insecticidal protein was found not only in cotton leaves, but also in the body and excrement of S. exigua and the bodies of both C. carnea and M. pallidipes. Bt toxin was detected in S. exigua larvae of all the examined instars (second, third, fourth, and fifth) that fed on transgenic cotton varieties and the Bt toxin level was the highest in the body of the second instar. In addition, the Bt toxin content in the excrement of the second instar was lower than that in the older ones. After the natural enemies C. carnea and M. pallidipes preyed/parasitised the S. exigua larvae that fed on transgenic cotton, Bt toxin was found in both the predator and parasite. This research indicates that Bt protein can be transferred through the food chain and to natural enemies of various predatory habits.

Efficacy of Transgenic Cotton Expressing Cry1Ac and Cry1F Insecticidal Protein Against Heliothines (Lepidoptera: Noctuidae)

2008 ◽  
Vol 101 (6) ◽  
pp. 1950-1959 ◽  
Author(s):  
M. Willrich Siebert ◽  
S. Nolting ◽  
B. R. Leonard ◽  
L. B. Braxton ◽  
J. N. All ◽  
...  
Keyword(s):  
Transgenic Cotton ◽  
Insecticidal Protein ◽  
Lepidoptera Noctuidae

Pyramiding CryIA(b) Insecticidal Protein and Terpenoids in Cotton to Resist Tobacco Budworm (Lepidoptera: Noctuidae)

1996 ◽  
Vol 25 (6) ◽  
pp. 1257-1266 ◽  
Author(s):  
E. S. Sachs ◽  
J. H. Benedict ◽  
J. F. Taylor ◽  
D. M. Stelly ◽  
S. K. Davis ◽  
...  
Keyword(s):  
Tobacco Budworm ◽  
Insecticidal Protein ◽  
Lepidoptera Noctuidae

Effects of the sap of the common oleander Nerium indicum (Apocyanaceae) on male fertility and spermatogenesis in the oriental tobacco budworm Helicoverpa assulta (Lepidoptera, Noctuidae)

2001 ◽  
Vol 204 (22) ◽  
pp. 3935-3942 ◽  
Author(s):  
Seong Eun Jeong ◽  
Yun Lee ◽  
Jeong Hee Hwang ◽  
Douglas C. Knipple
Keyword(s):  
Male Fertility ◽  
Chemical Constituents ◽  
Direct Injection ◽  
Arginine Decarboxylase ◽  
Tobacco Budworm ◽  
Polyamine Metabolism ◽  
Helicoverpa Assulta ◽  
Continuous Feeding ◽  
The Common ◽  
Lepidoptera Noctuidae

SUMMARY We investigated the effects of sap of the common oleander Nerium indicum (Apocyanaceae) on male fertility and spermatogenesis in the oriental tobacco budworm Helicoverpa assulta. We found that continuous feeding of oleander sap during the larval period significantly affects fertility in males but not in females. This effect was also induced by direct injection of oleander sap into the hemocoel of 2-day-old pupae. Histological analyses of developing testes following oleander injection revealed a developmental delay and progressively more severe morphological abnormalities in the later stages of development. The effects of oleander sap on spermatogenesis in H. assulta were associated with greatly reduced levels of the two major polyamines, spermidine and spermine, in testis compared with saline-injected controls. In contrast, levels of putrescine, which is a precursor of both spermidine and spermine, and the activities of the enzymes ornithine decarboxylase and arginine decarboxylase, which are involved in the biosynthesis of putrescine, were initially elevated following oleander injection, but subsequently failed to undergo the induction that normally occurs during late pupal development. The effects of oleander sap on spermidine and spermine levels could be the result of direct inhibition by chemical constituents of the oleander sap of one of the enzymes used in common in the conversions of putrescine to spermidine and spermidine to spermine; alternatively, these effects on polyamine metabolism could be secondary to the disruption of a more fundamental process in the developmental program guiding spermatogenesis in H. assulta.

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