Response of the Cottonwood Leaf Beetle (Coleoptera: Chrysomelidae) to Bacillus thuringiensis var. san diego

1990 ◽  
Vol 19 (2) ◽  
pp. 428-431 ◽  
Author(s):  
Leah S. Bauer
Keyword(s):  
Bacillus Thuringiensis ◽  
San Diego ◽  
Leaf Beetle ◽  
Cottonwood Leaf Beetle

scholarly journals Optimization of Cry3A Yields in Bacillus thuringiensis by Use of Sporulation-Dependent Promoters in Combination with the STAB-SD mRNA Sequence

1998 ◽  
Vol 64 (10) ◽  
pp. 3932-3938 ◽  
Author(s):  
Hyun-Woo Park ◽  
Baoxue Ge ◽  
Leah S. Bauer ◽  
Brian A. Federici
Keyword(s):  
Bacillus Thuringiensis ◽  
Type Strain ◽  
Culture Medium ◽  
Wild Type Strain ◽  
Expression System ◽  
Leaf Beetle ◽  
Wild Type ◽  
Additional Method ◽  
Chrysomela Scripta ◽  
Cottonwood Leaf Beetle

ABSTRACT The insecticidal activity of Bacillus thuringiensisstrains toxic to coleopterous insects is due to Cry3 proteins assembled into small rectangular crystals. Toxin synthesis in these strains is dependent primarily upon a promoter that is active in the stationary phase and a STAB-SD sequence that stabilizes the cry3transcript-ribosome complex. Here we show that significantly higher yields of Cry3A can be obtained by using dual sporulation-dependentcyt1Aa promoters to drive the expression ofcry3Aa when the STAB-SD sequence is included in the construct. The Cry3A yield per unit of culture medium obtained with this expression system was 12.7-fold greater than that produced by DSM 2803, the wild-type strain of B. thuringiensis from which Cry3Aa was originally described, and 1.4-fold greater than that produced by NB176, a mutant of the same strain containing two or three copies ofcry3Aa, which is the active ingredient of the commercial product Novodor, used for control of beetle pests. The toxicities of Cry3A produced with this construct or the wild-type strain were similar when assayed against larvae of the cottonwood leaf beetle,Chrysomela scripta. The volume of Cry3A crystals produced with cyt1Aa promoters and the STAB-SD sequence was 1.3-fold that of typical bipyramidal Cry1 crystals toxic to lepidopterous insects. The dual-promoter/STAB-SD system offers an additional method for potentially improving the efficacy of insecticides based onB. thuringiensis.

scholarly journals A P-Glycoprotein Is Linked to Resistance to the Bacillus thuringiensis Cry3Aa Toxin in a Leaf Beetle

Toxins ◽  
2016 ◽  
Vol 8 (12) ◽  
pp. 362 ◽  
Author(s):  
Yannick Pauchet ◽  
Anne Bretschneider ◽  
Sylvie Augustin ◽  
David Heckel
Keyword(s):  
Bacillus Thuringiensis ◽  
Leaf Beetle ◽  
P Glycoprotein

THE BIOLOGY AND ECOLOGY OF THE COTTONWOOD LEAF BEETLE, CHRYSOMELA SCRIPTA (COLEOPTERA: CHRYSOMELIDAE), ON TISSUE CULTURED HYBRID AIGEIROS (POPULUS × EURAMERICANA) SUBCLONES IN WISCONSIN

1979 ◽  
Vol 111 (5) ◽  
pp. 551-556 ◽  
Author(s):  
T.R. Burkot ◽  
D.M. Benjamin
Keyword(s):  
Biological Control ◽  
Leaf Beetle ◽  
Coleomegilla Maculata ◽  
Third Generation ◽  
Biological Control Agents ◽  
Developmental Threshold ◽  
Populus Euramericana ◽  
The Third ◽  
Chrysomela Scripta ◽  
Cottonwood Leaf Beetle

AbstractAdults and larvae of the cottonwood leaf beetle, Chrysomela scripta Fab., defoliated tissue cultured Aigeiros (Populus × euramericana (Dode) Guinier) subclones and destroyed apical tips. Fecundity was 510 ± 153 eggs . Four generations occurred between May and September in southern Wisconsin. Maximal insect numbers and damage occurred in the third generation. The minimal developmental threshold was 10.8 °C with a mean 257 ± 26 day-degree (°C) required per generation. Important biological control agents included Coleomegilla maculata, which consumed up to 25% of the eggs, and Shizonatus latus, a pteromalid parasite, which destroyed up to 26% of the pupae.

An Artificial Diet for Cottonwood and Imported Willow Leaf Beetles (Coleoptera: Chrysomelidae) and Comparative Performance on Poplar Foliage2

1990 ◽  
Vol 25 (3) ◽  
pp. 475-480 ◽  
Author(s):  
Leah S. Bauer ◽  
Joann Meerschaert ◽  
O. Thomas Forrester
Keyword(s):  
Artificial Diet ◽  
Leaf Beetle ◽  
Adult Longevity ◽  
Comparative Performance ◽  
Laboratory Rearing ◽  
Leaf Beetles ◽  
Chrysomela Scripta ◽  
The Media ◽  
Cottonwood Leaf Beetle ◽  
Willow Leaf Beetle

An artificial diet was developed for laboratory rearing of the cottonwood leaf beetle, Chrysomela scripta F., and the imported willow leaf beetle, Plagiodera versicolora (Laicharting). To reduce microbial contamination of the media, procedures were developed for separating egg masses and sterilizing egg surfaces. Cottonwood leaf beetle larvae reared from neonate to adult on this artificial diet had greater mortality, took longer to develop, and were smaller than larvae reared on fresh poplar foliage. Adult longevity was similar for both diet-and foliage-reared larvae. Survival and adult fresh weight of imported willow leaf beetle larvae reared on the artificial diet were similar to those of cohorts reared on fresh poplar foliage. However, individuals reared on artificial diet took longer to develop and produced shorter-lived adults than cohorts reared on foliage. Larvae of both species would not eat fresh foliage after being fed on the artificial diet. Adults of both species maintianed on the artificial diet laid few eggs but resumed normal oviposition when fed fresh foliage. This artificial diet proved useful for rearing larvae and maintaining adults during periods when fresh foliage was limited.

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